ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10401.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10402.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10403.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1997.50303.x-i6

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

In vitro studies have shown that platelet‐derived growth factor binds to specific receptors on osteoblasts and induces proliferation. Some reports also indicate that platelet‐derived growth factor may inhibit differentiation of bone cells. Such findings are difficult to interpret, however, because many of these studies used impure platelet‐derived growth factor extracted from platelets (rather than pure, recombinant platelet‐derived growth factor), used mixed cell populations, and added serum to the culture media (likely introducing additional growth factors). In vivo studies also have yielded conflicting results regarding the effects of platelet‐derived growth factor on bone formation. Platelet‐derived growth factor may act in concert with insulin‐like growth factor I to induce bone formation in a canine periodontal system. Other investigators, however, have shown that platelet‐derived growth factor inhibits the effects of osteoinductive proteins such as osteogenin. No evidence of bone formation has been detected in patients receiving platelet‐derived growth factor treatment of soft tissue wounds overlying bony prominences in clinical trials. Thus, despite the observations that osteoblasts can specifically bind and proliferate in response to platelet‐derived growth factor in tissue culture, platelet‐derived growth factor alone has not yet been proved to be osteoinductive in vivo. These observations suggest that in the presence of the many cytokines and multiple cell types found in wounds and fractures, the direct effects of platelet‐derived growth factor on o

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10405.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

A total of 67 patients with pressure ulcers were randomized into one of three treatment modalities: hydrogel sheet dressing, hydrocolloid, or wet‐to‐moist gauze. Safety, efficacy, and physical attributes of the three dressings were evaluated. No statistical significance was found in wound healing rate among the three treatments. Hydrogel sheets were advantageous in allowing wound visualization without dressing or wound disrupt

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10406.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

The purpose of this study was to localize transforming growth factor‐α, a mitogenic peptide, and its receptor, epidermal growth factor receptor, to correlate these findings with epithelial cell proliferation in human burn wounds. Proliferating cell nuclear antigen, a marker of cellular proliferative activity, was used in conjunction with the immunolocalization of transforming growth factor‐α and epidermal growth factor receptor in a series of partial‐ and full‐thickness burn wounds 2 to 22 days after injury. Identification of the proliferating population by proliferating cell nuclear antigen nuclear labelling indicated that both ligand and its receptor are concurrently present in proliferating epidermis. The simultaneous, intense localization of transforming growth factor‐α and epidermal growth factor receptor during the early, intermediate, and late postburn period lends further support to an epidermal growth factor/transforming growth factor‐α/epidermal growth factor receptor—mediated growth repair mechanism. Furthermore, the distribution of transforming growth factor‐α and epidermal growth factor receptor in nonproliferating populations within healing burn wounds suggests additional multifunctional roles for this growth factor pathway during human cu

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10407.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

Scatter factor—hepatocyte growth factor is a protein secreted by fibroblasts which disperses colonies of epithelial cells and keratinocytes in culture. The factor is also a patent mitogen for hepatocytes, synthesized in the liver. Basic fibroblast growth factor, another heparin‐binding factor, is most abundant in the brain but also plays a role in wound healing. Using a solution hybridization/RNAase protection assay, we have measured the abundance of messenger RNA for scatter factor—hepatocyte growth factor and basic fibroblast growth factor in granulation tissue obtained from subcutaneously Hunt‐Schilling wound cylinders. The levels of scatter factor—hepatocyte growth factor messenger RNA increased after weeks 2 through 4 to a twofold higher level in weeks 5 through 7 after implantation of the cylinders, whereas no changes in basic fibroblast growth factor messenger RNA levels were noticed. At week 3 after implantation of the cylinders, scatter factor—hepatocyte growth factor messenger RNA levels in granulation tissue were more than threefold higher than in skin dermis fibroblasts but markedly lower than in the liver. The abundance of basic fibroblast growth factor messenger RNA was also significantly increased in granulation tissue compared with dermis but, as expected, markedly lower than in the brain. In conclusion, the gene expression of the scatter factor—hepatocyte growth factor, as well as basic fibroblast growth factor, is increased in granulation tissue. Because there was a time‐dependent increase in the expression of scatter factor—hepatocyte growth factor, it is hypothesized that scatter factor—hepatocyte growth factor acts as a signal from fully developed granulation tissue to stimulate skin epithelial cells to sca

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10408.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

The intravascular actin scavenger system depolymerizes and sequesters actin released after tissue injury. Studies were carried out to determine if this system is active in the extracellular space during wound repair. Using burn wound fluid as a noninvasive means for analyzing the wound environment, we measured actin accumulation and actin complex formation with the plasma proteins responsible for scavenger function. Actin at concentrations as high as 0.25 mg/ml (∼5 µmol/L) was found in burn wound fluid samples from 9 of 11 patients. Wound fluid also contained the two plasma proteins that bind actin—gelsolin (both plasma and cytoplasmic forms) and Gc protein. Because actin in wound fluid was complexed with gelsolin and Gc protein, we conclude that the components of the actin scavenger system are functional in wound tissue. In addition, proteolysis of gelsolin, but not actin or Gc protein, appeared to occur at the wound site. Gelsolin proteolysis was accompanied by the appearance of 49 kd gelsolin fragments, and wound fluid samples lacking intact gelsolin also contained high metalloproteinase le

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10409.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

We tested different biodegradable matrix materials as dermal substitutes in a porcine wound model. Matrixes were covered with a split‐skin mesh graft and protected with a microporous, semipermeable membrane, which prevents blister formation, wound infection and provides ultimate healing conditions. Evaluation parameters were as follows: epithelization, dermal reconstitution, wound contraction, and cosmetic and functional aspect. A microfibrillar matrix of nondenatured collagen gave the best result, with immediate fibroblast ingrowth and epidermal outgrowth. Slight inflammatory reaction and minimal wound contraction were observed. Application of a split‐skin mesh graft, in combination with this collagen matrix, generated a thicker dermal layer than did a split‐skin mesh graft directly applied on a wound bed. However, the histologic dermal architecture was less optimal than one obtained with a full‐thickness punch graft method. Other matrixes caused inflammatory reactions, interfering with epithelization and dermal reconstitution. We conclude that a nondenatured collagen matrix, in combination with a split‐skin mesh graft, can provide a substitute dermis in a full‐thickness wound. This combination is preferable to a split‐skin mesh graft directly applied on the wound bed. With our microporous semipermeable membrane, the combined use of a dermal substitute and a split‐skin mesh graft can be applied in a single

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10410.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY