摘要:

A simple gadget is described for the safe and rapid mounting of cover slips on slides with infectious materials. The instrument has worked satisfactorily in the mounting of more than 50,000 slides.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02607.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Quantitative recovery by swab cultures ofStaphylococcus aureusorEscherichia colifrom experimental wounds in pigs was studied for assessment of wound contamination in elective surgery. The recovery rate declined rapidly over the first hour. Ten minutes after seeding the wounds with known numbers of bacteria, the recovery was respectively 0.7% and 4.1% forS. aureusandE. coli.Recovery was not affected by the size of the wound. Streaking the swab onto the culture plate, or rinsing the swab in saline and plating out the rinsing fluid immediately after sampling resulted in a three to four times higher yield than by using Stuart's transport medium for later plating. The results provide an indication of the discrepancy between the number of micro‐organisms recovered from wounds in elective surgery and the number of micro‐organisms required to produce suppurative infection in experiments on animals and in

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02608.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Hairsamples of domestic and laboratory animals suspected of dermatophytosis were examined for the presence of dermatophytes. A nutritionally poor base‐medium developed by the author was successfully used in the isolation and identification of dermatophytes. Casein‐medium supplemented with vitamins and Sabouraud‐liquid medium were used in special cases. Dermatophytes were isolated in 36 of 331 samples (10.9%). The dermatophytes recovered wereMicrosporum canis: 13 isolates from cat. 4 from dog, 1 from horse;Trichophyton mentagrophytes var. granulare: 3 isolates from dog, 3 from horse. 2 from guinea pig and 1 from rabbit;Trichophyton terrestre1 isolate from dog. Eleven of the 13 feline isolates originated from house cats and the relative frequency was higher among the purebred cats. Two of the cat isolates were connected with human dermatophy

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02609.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

A new indirect (passive) haemagglutination technique (Rubacell, Abbott) has been evaluated on the basis of a material of 1,792 sera sent for serological diagnosis of a rubella infection or determination of immunity status. The test procedure is very simple. IHA antibodies appeared about two weeks later than HI antibodies and seemed to persist for life. Two fields of application for the IHA technique are discussed: 1. As a supplement to other tests because of the late appearance of the antibodies;2. In screening for immunity status. The IHA combines ease of performance with high sensitivity.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02610.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The fatty acid composition of 35Haemophilus influenzaestrains was found to be grossly similar and characterized by relatively large amounts of 14:0, 3‐OH‐14:0, 16:1 and 16:0. The three C18fatty acids 18:2, 18:1 and 18:0 were also present, but in much lower concentrations. This general pattern was also found for most of the other species ofHaemophilusexamined (H. aegyptius, H. aphrophilus, H. canis, H. gallinarum, H. haemolyticus, andH. parainfluenzae). Small but distinct quantitative discrepancies were detected forH. ducreyiand the haemin‐independent speciesH. paraphrohaemolyticus, H. paraphrophilusandH. suis. Actinobacillus actinomycetemcomitanswas found to be indistinguishable fromH. influenzae. Pasteurella multocidaalso exhibited a fatty acid pattern closely related to that ofHaemophilus, but could be distinguished by its higher concentration levels of the C18fatty acids. The fatty acid pattern ofH. vaginaliswas considerably different from those of the other species examined. This species lacked 3‐OH‐14:0 and 18:2 and contained small amounts of 14:0 and 16:0, whereas 18:1 and 18:0 were the major con

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02611.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Enterotoxin fromClostridium perfringenswas shown to retain its biological activity after labelling with125I. When injected intravenously into mice and rats, most of the radioactivity in the organs was present in the form of intact toxin. Studies of the tissue distribution of labelled enterotoxin showed the largest amounts in the liver, where the activity reached a maximum 10–15 min after administration. The highest concentration per g tissue was found in liver and kidneys. The radioactivity was excreted in the urine as a mixture of intact labelled toxin and low molecular weight degradation products.In vitrostudies with purified parenchymal liver cells showed rapid release of lactate dehydrogenase (LDH) during treatment with enterotoxin, thus indicating severe membrane damag

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02612.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Gentamicin, trimethoprim, cephalothin, colistin, erythromycin, oxytetracycline and chloramphenicol were studied in a phagocytic system. A radiolabelled strain ofE. coliwas used as test bacterium and human polymorphonuclear cells were used as phagocytes. Except for trimethoprim and cephalothin, there was a tendency towards depression of the process of phagocytosis in the presence of high concentrations of the various antibiotics. Colistin in a high concentration (83 μg/ml) exerted the most significant effect

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02613.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Experimental infection withChlamydia trachomatis, immunotype K, was carried out in six grivet monkeys. Three routes of infection were used: (A) into the uterine cavity through the cervical canal: (B) into the uterine cavity, but after ligature of the tubal isthmus, followed by curettement of the endometrium: (C) into the cervical epithelium. Two monkeys were used for each type of experiment. All monkeys inoculated by routes (A) and (C) developed marked signs of inflammation in the Fallopian tubes. Characteristically, the tubes were reddened and swollen. Exudate appeared in the abdominal ostia and in the cul‐de‐sac. In three of the four monkeys studied, a yellowish discharge was seen in the posterior vaginal fornix. An exudate composed mostly of lymphocytes and clusters of desquamated cells was found in the lumen of the tubes. The epithelium was injured in some areas and there were adhesions between the mucosal folds. In two of the monkeys, tubal occlusion was present two months after the inoculation. With one exception, all monkeys presented a significant rise in antibodies toC. trachomatis.In the two monkeys inoculated by route (B), no signs of inflammation in the upper genital tract were seen, but one of the animals died day 14 post inoculation;autopsy showed peritonitis and perihepatitis. One monkey was inoculated with non‐infected yolk sac specimen by route (A) and another by route (B). Practically no inflammatory changes in the genital organs or elsewhere could be detected in these monkeys. The results indicate thatC. trachomatiscan cause salpingitis in female grivet monkeys by canalicular spread from the lower genital tract. The histological findings and the infection route in this experimentally induced salpingitis in the grivet monkeys closely resemble those ascribed to «gonococcal« salpingitis

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02614.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Lipopolysaccharides (LPS) extracted from cells ofFusobacterium necrophorum, F. mortiferum, F. gonidiaformans, F. variumand single strains ofF. naviformeandF. russiwere analysed for sugars and fatty acids. All preparations contained a monosaccharide tentatively identified as L‐glycero‐D‐mannoheptose, but showed variation with respect to the presence of glucose, galactose, rhamnose and another heptose isomer, tentatively identified as L‐glycero‐D‐mannoheptose. L‐glycero‐D‐mannoheptose was a monosaccharide of great abundance in all LPS examined. 2‐keto‐3‐deoxy‐octonate and glucosamine were present in all LPS preparations. The fatty acids present were 3‐hydroxy‐tetradecanoic acid as the main component, andn‐tetradecanoic acid. Some LPS containedn‐hexadecanoic acid. All the strains ofF. nucleatumcontained 3‐hydroxy‐hexadecanoic aci

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02615.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The wall teichoic acid ofStaphylococcus simulanshas been characterized as a glycerol phosphate polymer with glycosidically linked N‐acetylglucosamine. Susceptibility toβ‐N‐acetylglucosaminidase and serological similarity to poly CβfromStaphylococcus saprophyticus, showed that the amino sugar is in theβ‐con

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1980.tb02616.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY