摘要:

If only conventional laboratory tests are used, the widely occurring speciesPseudomonas cepaciamay, due to its variability, sometimes be difficult to differentiate from the dangerous pathogenPseudomonas pseudomallei.In a comparative study using fresh isolates it is shown that animal inoculation seems to be a useful additional method in the differentiation. Also the determination of the ability to utilize selected carbon sources such as starch, hydroxybenzoate and uracil is helpful in reaching a correct identification.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00073.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The growth of feline panleukopaenia virus was examined in relation to various cell growth curves of secondary feline kidney cells. In cell cultures with a high mitotic index, the manifestation of virus infection, expressed as percentage number of cells with inclusion bodies, was correspondingly high. In cultures with low mitotic index, the percentage number of cells with inclusion bodies was low. A cytopathic effect was seen by inoculation of virus with a titre of 103.5TCID50/ml. This effect was most pronounced using cell cultures seeded in a quantity of 1 million cells per ml to 500,000 cells per ml with inoculation made during the first 24 hours after seeding when the mitotic index was high. Both the cytopathic effect and the occurrence of inclusion bodies were of transient nature. The peak of infection, both with regard to the cytopathic effect and the percentage number of cells with inclusion bodies, was seen three days after inoculation.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00074.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The immunoperoxidase technique together with electron microscopy has been examined for the ultrastructural localization of staphylococcal protein A, the immunoferritin method being included for comparison. The results show that protein A is uniformly distributed in the whole cell wall. Both the direct and indirect methods, Fab‐ as well as Fc‐reactions, showed identical results. The immunoperoxidase method was superior to the immunoferritin method, especially when applied to thin sections of the bacteria, and the clear specificity demonstrated indicates a useful method for localization of cellular antig

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00075.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Cells ofTreponema genitaliswere studied in the electron microscope by means of negative staining and ultrathin sectioning techniques. All cells were covered by a regularly structured surface layer. This layer appeared to consist of pairs of thin fibrils attached to an amorphous layer. This amorphous layer in turn is probably identical with the exterior part of the outer membrane of the organism. The pairs of thin fibrils located on this surface were interconnected by polygons. The treponemes were regularly coiled and had somewhat tapered ends with 2–4 flagella inserted at each end. The two bundles of flagella were entwined around the cytoplasmic body of the cell and interdigitated in the middle region of the organism. Treatment of cells ofT. genitaliswithMyxobacterAL‐1 protease 1, or with deoxycholate did not reveal intracytoplasmic tubules. This is in contrast to the results obtained with similar treatments of all other strains of species ofTreponemahitherto exami

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00076.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The influence of therapeutic concentrations of phenylbutazone on human granulocyte function has been examined using a method which facilitates a precisein vitroevaluation of the phagocytic and bactericidal activities of polymorphonuclear leucocytes. Phenylbutazone caused a marked reduction in intracellular killing of bacteria by the granulocytes. Whether this inhibition of granulocyte function also takes placein vivoresulting in enhanced susceptibility to infection, remains unknown.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00077.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Negatively stained preparations of 16 strains ofAcinetobacter calcoaceticuswere examined in the electron microscope. Five of the strains did and 11 of the strains did not exhibit twitching motility. Two of the non‐twitching strains were substrains of twitching wild‐type strains. All twitching strains were found to possess fimbriae with a diameter of approximately 50 Å These fimbriae were shown to be of polar origin except in one strain where, for technical reasons, the origin could not be determined with certainty. Polar fimbriae could not be demonstrated in any of the strains that did not exhibit twitching motility. The demonstration of polar fimbriae was only regularly possible during the exponential growth phase, presumably because the fimbriae are shed by the bacteria during later growth phases. During the study, methods were developed for securing exponentially growing bacteria in concentrations suited for the preparation of negatively stained cells for electron microscopy. Peritrichously arranged fimbriae with a diameter of approximately 30 Å were demonstrated in nearly all strains studied, especially on cells in late growth phases. The occurrence of this kind of fimbriae was therefore not correlated with the occurrence of twitching mot

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00078.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

FourEscherichia colistrains were established as antigenic test strains for four new O groups, O158, O159, O160 and O161. The strains of O158 and O159 were isolated from cases of infantile diarrhoea in Great Britain, while the O160 and O161 strains were isolated from faecal specimens in Arabia. The O161 strain was at the same time designated as test strain of a new H antigen, H54, while a strain belonging to O group 148 was established as antigenic test strain for H53; this strain was also isolated from human faeces in Arabia.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00079.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

ThreeE. colistrains representing strains from faeces of normal persons were established as antigenic test strains of two new O antigens, O162 and O163, and one new H antigen, H56.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00080.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

57 dialysis patients, 29 of whom had been exposed to hepatitis in 1968‐69, were re‐examined for hepatitis B antigen (HB‐Ag) and antibody (HB‐Ab) by means of radioimmunoassay (RIA) and counter immunoelectrophoresis (CIE). Infected patients were followed for five years. Compared to CIE, RIA did not detect additional HB‐Ag‐positive patients, but antibody could persistently be detected in seven out of fifteen patients previously supposed to have escaped infection. Nine surviving HB‐Ag‐positive transplant patients remained carriers for 50–72 months. None developed clinical signs of chroni

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00081.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A strain identified asPasteurella multocida(“dog type”) grew on blood agar with spreading and corroding colonies; it was found to be fimbriated. A non‐fimbriated variant of the strain which did not produce spreading and corroding colonies was isolated. The fimbriated strain regularly formed surface growth by static incubation in broth. The mode of surface translocation of this strain appeared to be twit

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00082.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY