摘要:

The endogenous stages in the life cycle ofEimeria brunettiwere tested for polysaccharide material by electron microscopical cytochemistry using the periodic acid‐thiocarbohydrazideosmium tetroxide (PATO)‐method. The parasites were observed within the epithelial cells of the small intestine of infected domestic fowls. Two types of granules reacted positively for polysaccharide. The first was large, approximately 500 nm by 250 nm. These granules had an appearance similar to the polysaccharide granules reported for other coccidian parasites. They were observed in mature merozoites, macrogamonts, and developing oocysts. The second type of granule was smaller (15–30 nm in diameter) and was only observed at the periphery of the residual cytoplasmic mass of mature microgamonts. It was more similar in appearance to metazoan glycogen than the former. However, since the PATO‐method does not differentiate between different polysaccharides it can not be proven if these granules in fast contain glycogen. The WFB I of the macrogamete and the outer layer of the oocyst wall gave a slight positive reaction but after examination of the controls it appeared that this was not a specific reaction for polysacc

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1977.tb01969.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Optimal conditions for acridine orange staining of air dried and methanol fixed bacteria on glass slides were studied. The pH of the staining buffer did not influence the fluorescence of anS. aureusand anE. colistrain at dye concentrations of 25–50 mg per litre. 81 bacterial strains representing 15 different species were stained with acridine orange under standard conditions, all strains showing orange fluorescence. The pH of the buffer influenced markedly the staining patterns of human cells and tissue materials, as represented by smears of peripheral blood, buccal scrapings, urethral secretions and tracheal exudates. The fluorescence obtained ranged from low intensity green at low pH values to bright orange at neutral and alkaline pH. This variability indicated a possibility of designing conditions for a differential staining method for the detection of bacteria in clinical specimens. The differential staining effect with a low pH in the buffer was confirmed on smears of buccal scrapings, cerebrospinal fluid samples and urethral secretions, showing orange fluorescence of the bacteria present and green‐to‐yellow fluorescence of background material, cells and tissue d

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1977.tb01970.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Lysolecithin exhibits a lytic activity on acholeplasmas and mycoplasmas. The acholeplasmas studied, viz.Acholeplasma laidlawiiA and B, were found less susceptible than were the mycoplasmas, viz.Mycoplasma gallisepticumandM. pneumoniae.The sensitivitxy to lysis was found to differ according to species, growth temperature and number of organisms used. Variations in age of the population and the concentration of bivalent ions in the test medium had but little influence. The greatest lytic activity of lysolecithin was found at the optimal growth temperature and decreased with lowering of the temperature. This lytic activity was inversely proportional to the density of the cell suspensions used. The possible mechanism of the lytic effect of lysolecithin is discussed.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1977.tb01971.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Lipopolysaccharides (LPS) extracted from strains ofBacteroides fragilis, Bacteroides melaninogenicusandBacteroides oraliswith phenol‐water, trichloroacetic acid, EDTA or liquid phenolchloroform‐petroleum ether (PCP) and isolated by ultracentrifugation, varied considerably in their quantitative chemical composition. Negligible yields of LPS were obtained by PCP‐extraction. All preparations were more or less serologically active. All methods (except PCP) extracted the same O‐antigenic determinants fromB. fragilis.Endotoxic activity, as measured by primary skin inflammations in rabbits, was low but was present in all preparations. Proteins (and/or lipoproteins) co‐precipitated with LPS in the ultrac

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1977.tb01972.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

The instestinal β‐glucoronidase was studied in germfree, monocontaminated and conventional rats. The greater part of the β‐glucuronidase of the caecum and the large intestine of the contaminated animals was of bacterial origin. No bacterial β‐glucuronidase was found in the small intestine. Monocontamination withEscherichia coligave activities corresponding to those of the conventional rats, whereas content from the caecum and the large intestine of the rats monocontaminated withStreptococcus pyogenesshowed an activity approximately 10 per cent of that of the conventio

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1977.tb01973.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

The production of staphylococcal serineproteinase (Protease I) in 170Staphylococcus aureusstrains from human and bovine clinical isolates was investigated. Protease was identified in 57 per cent of the strains by electroimmuno assay.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1977.tb01974.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Colonies of Ureaplasma urealyticum serotype VI grown on agar were examined by thin sectioning technique. The arrangement of cells within the colonies was less dense and less stratified than that observed in most other mycoplasma colonies. Small, dense and pleomorphic cells were located mainly along the colony borderline towards the agar. Studies of cell morphology after 3, 10 and 15 days of growth revealed that such cells were younger than the less dense, mostly ovoid cells seen in the central area of colonies. The ultrastructure of individual cells was similar to that ofUreaplasmagrown in liquid medium, as described earlier, featuring geometrical ribosome arrangements and extramembranous coat. Serial sections demonstrated the difficulties involved in discussing cell morphology on the basis of single thin sections. The advantages of the preparation method used for electron microscopy are discussed.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1977.tb01975.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Most haemolytic group B streptococci produced a yellow to orange pigment in Colombia agar and in some Todd Hewitt broths. The composition of the broth medium was important. Trypsin inhibited pigment production, but had no effect on pigment already produced. Optical density measurement: revealed three main peaks at 420, 260 and 215 nm respectively with pigment producing strains. Nonpigmented strains gave only the two peaks at 260 and 215 nm.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1977.tb01976.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY