摘要:

Histological findings in 75 children with polycystic kidney disease were analysed. The patients had been divided into recessive (RPKD), dominant (DPKD), and sporadic cases on the basis of family investigations. The histological findings in the patients with RPKD and DPKD were compared with each other in order to define criteria for classificastion of the sporadic patients. The kidneys of the neonatally‐deceased patients with RPKD (26 patients) and DPKD (4 patients) were macroscopically very similar. On histological investigation, all patients with RPKD (32 patients) showed cysts derived from the collecting ducts. In DPKD, the cyst epithelium was more variable, and the presence of glomerular cysts served as a useful histologial marker of this disorder. In RPKD there was diffuse biliary dysgenesis, in contrast to the normal liver in the neonatally‐deceased DPKD patients. However, one DPKD patient who survived also had biliary dysgenesis. Using the observed differences between RPKD and DPKD as criteria, all sporadic patients with satisfactory histological material could be classified as RPKD. We conclude that the differential diagnosis between RPKD and DPKD can be based on kidney and liver histol

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05270.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The effect of serum from both type I and type II diabetic subjects on the metabolism of arterial basement membrane (BM)‐like material was studied in cultures of rabbit aortic smooth muscle cells. The basement membrane‐like material was isolated from the cell‐layer by a combined sonication and centrifugation technique. Serum from type I diabetic persons added to the incubation medium increased statistically significantly the incorporation of L‐[4,5]‐3H‐leucine into the basement membrane‐like material as compared to serum from non‐diabetic subjects (2P<0.05). The same effect was seen with serum from type II diabetic patients as compared to serum from nondiabetic subjects (2P<0.05). No effect of serum from type I diabetic persons was seen in degradation experiments. Incubation medium supplemented with normal serum and extra glucose neither changed the production of basement membrane‐like material nor the disappearence rate of radioactive leucine from the basement membrane‐like material in degradation experiments. The present study indicates that serum from diabetic subjects enhances the production of arterial basement membrane‐like material from arterial smooth muscle cells in culture. The obtained data may be relevant for the understanding of the development of macroangiopathy am

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05271.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Campylobacter pyloriis supposed to be involved in the pathogenesis of gastroduodenal peptic ulcer diseases and chronic gastritis. In order to study whether theCampylobacter pyloriin the stomach of peptic ulcer patients is related to ulcer itself or to a co‐existing chronic gastritis, we examined the frequency of the bacteria in Giemsa stained histological sections of biopsy specimens from a series of patients with active peptic ulcer and from series of non‐ulcer control subjects. We found no difference in the frequency of Campylobacter‐ positive cases between ulcer patients and non‐ulcer controls when the comparison was done within the same category of chronic gastritis; e.g., within the category of chronic superficial gastritis 74% and 78% of cases showed the bacteria in antral biopsies from ulcer patients and from non‐ulcer controls, respectively. In both ulcer patients and control subjects, in similar way in both antral and body mucosa, theCampylobacter pyloriwas strongly associated with chronic superficial gastritis but was more weakly associated with chronic atrophic gastritis, and the bacteria were only occasionally seen in normal mucosa. We conclude thatCampylobacter pyloriis associated with chronic gastritis in peptic ulcer patients but is not related to act

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05272.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Endotoxin‐stimulated human peritoneal macrophages were cultured in serum‐free medium with agarose beads. Monospecific antibodies to human C3c, C3g, C5, C6, C7, C8, C9 and to C9‐neoantigen bound to the beads. This shows that activated C3 and the terminal complement complex (TCC), made from complement components C5 to C9, were generated on the beads.De novosynthesis was confirmed by agarose binding of tritium‐labelled protein. Moreover, C3‐derivatives and C9‐neoantigen were detected on normal serum‐treated agarose beads but not on beads kept in factor B‐depleted or heat‐inactivated sera, implying that an intact alternative complement pathway was required for our findings. The macrophages thus synthesize the active complement components of the alternative and terminal

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05273.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

It has been found in several different test systems that LPS is competent to induce autoantibodies of different specificities. As a rule only IgM autoantibodies have been found. The presence of autoreactive B cells in healthy individuals indicate that autoreactive B cells have not been not eliminated by the induction of immunological tolerance. This result is to expected according to the concept of the one non‐specific signal hypothesis for B cell activation, which only ascribes a passive focussing role to the immunoglobulin receptors, whereas activation and tolerogenic signals are delivered via non‐clonally distributed receptors for polyclonal B cell activators and T cell interleukins. The induction of autoantibodies by LPS in mice does not result in detectable disease or symptoms. Therefore, the ability of LPS to induce IgM autoantibodies does not constitute a model for induction of autoimmune diseases. The main importance of these findings is that the self‐non‐self distinction is not carried out by B cells. Presumably T cells alone are responsible for the self‐non‐self

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05274.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Spontaneously hypertensive rats (SHR) and stroke‐prone spontaneously hypertensive rats (SHRSP) were treated with a combination of a beta1‐blocker (metoprolol) and a calcium antagonist (felodipine) from 1 to 4 or from 4 to 6 months of age. Basal cerebral arteries were fixed by immersion and embedded in plastic. The ratio between media thickness and luminal radius was determined in cross‐sectioned arteries for a standardized condition, assuming a smooth and circular internal elastic membrane. The treatment caused a significant decrease in blood pressure and a normalization of m/r ratios in basal cerebral arteries when initiated in young as well as in adult SHR and SHRSP, i.e. the therapy efficiently prevents as well as reverses hypertensive arterial changes. Even though 4‐month‐old treated rats were still significantly hypertensive, their m/r ratios did not differ from those of normotensive controls. The latter results may indicate that the treatment affects the vessel walls also through other mechanisms than by decreasing the pres

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05275.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Restriction enzyme (RE) patterns, using Hind III enzyme, were analysed on 101 urogenital strains ofNeisseria gonorrhoeae.The strains were also analysed serologically, using monoclonal antibodies, and tested for nutritional requirements by auxotyping. In addition, testing susceptibility to doxycycline was performed by an agar‐dilution method. The strains were distributed into 23 RE patterns, of which the five most common accounted for 67.3% of all isolates. The same strains were distributed into 17 serovars, of which the four most common accounted for 77.3% of all isolates. Eleven auxotypes were demonstrated, of which the four most common accounted for 85.1% of all isolates. When the methods were combined, 38 combinations of RE patterns and serovars, 25 combinations of RE patterns and auxotypes, and 31 combinations of serovars and auxotypes were seen. Combining all three methods, 40 combinations of RE patterns, serovars and auxotypes were found. Correlations were particularly seen between RE patterns and auxotypes. The strains were moderately sensitive to doxycycline, those with serological markers for WII/WIII being less sensitive than those with markers for W

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05276.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Aggregates of nevus cells in the axillary lymph nodes may give rise to a suspicion of metastatic breast cancer. Usually the nevus cells are confined to the capsule or the trabeculae, but in the present case, clusters of nevus cells were also observed in the peripheral sinus. Immunohistochemical analysis for S‐100 protein and epithelial membrane antigen established the true nature of the cell

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05277.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A histologically‐confirmed, multicystic, benign mesothelioma, with free‐floating, thin‐walled cysts, in the abdominal cavity of a 27‐year‐old woman was reported in 1954. After removal of all visible cysts by laparotomy, the patient was healthy and well for 29 years, when she was surgically treated for cholecystitis and gall bladder stones in 1982. The whole peritoneum was found covered with small cysts lined by mesothelial cells. The patient is (April 1987) well, with no complaints. Sections from the old paraffin blocks were studied by means of scanning, transmission electron microscopy and immunohistochemistry. These methods confirmed the histological diagnosis. The authors discuss whether such a lesion really is a benign tumor or should rather be otherwise c

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05278.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Campylobacter pyloriwas isolated more often from patient with peptic ulcers and in an age‐related manner in a material of 395 consecutive patients referred for gastroscopy. Direct microscopy was done with Gram and Acridine Orange stain and found too insensitive for practical use. All patients were investigated serologically with an enzyme immunoassay which showed excellent correlation with positive cultures forC. pylori.The findings were discussed, and the enzyme immunoassay, with a negative predictive value of 0.99, was found to be a valuable tool for primary screening of patients suspected of being carriers ofC. pylor

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1988.tb05279.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY