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81. |
Effect of PGE2and LTB4on vicia villosa binding lymphocytes |
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APMIS,
Volume 96,
Issue 1‐6,
1988,
Page 531-536
NORBERT GUALDE,
JEANNE‐MARIE COOK,
FABIENNE GUIBERT,
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摘要:
Since there is a good deal of evidence that vicia villosa lectin (VVA) binds to contrasuppressor cells, mouse splenocytes and thymocytes were sorted by binding to VVA‐coated Petri dishes. It was observed that vicia villosa adherent cells (VVA(+)) did not proliferate when mitogens were added to cultures, but they did enhance the thymidine uptake of PHA or ConA stimulated vicia villosa non‐adherent (VVA(‐)) splenocytes. PGE, treatment of VVA(+) splenocytes or VVA(+) immature thymocytes did not very much affect the VVA(+) cell behavior. On the other hand, LTB4increased the enhancing capability of VVA(+) splenocytes. Therefore, investigations dealing with the effects of LTB4on lymphocyte subsets which may include VVA(+) cells should take into consideration the possible presence of contrasuppressor
ISSN:0903-4641
DOI:10.1111/j.1699-0463.1988.tb05340.x
出版商:Blackwell Publishing Ltd
年代:1988
数据来源: WILEY
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82. |
Glucagon and glucagon‐like immunoreactive cells in mid‐ and hindgut carcinoids |
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APMIS,
Volume 96,
Issue 1‐6,
1988,
Page 537-542
M. EL‐SALHY,
E. WILANDER,
M. LUNDQVIST,
G. NILSSON,
L. GRIMELIUS,
A. J. MOODY,
K. IMAGAWA,
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摘要:
The occurrence of glucagon/glucagon‐like immunoreactivity in 31 small intestinal, 34 rectal and 18 appendiceal carcinoids were investigated immunocytochemically using, sequence specific antisera. Glucagon/GLI immunoreactive cells were found in five small‐intestinal and five rectal carcinoids, but none were observed in any of the appendiceal carcinoids examined. Glucagon/GLI immunoreactive cells constituted a minor cell population, except in one rectal carcinoid, where most of the tumour cells were of this type. Glucagon/GLI immunoreactive cells were detected with only some sequence‐specific antisera, and not with antisera directed against the rest of the glucagon/glicentin molecule. This might indicate that these cells contain a molecule which shares some antigenic binding sites with glucagon/glicentin rather than genuine glucagon/glicentin. it is concluded that this finding contributes to explain why hindgut carcinoids rarely give rise to symptoms related to neuro‐endocrine pro
ISSN:0903-4641
DOI:10.1111/j.1699-0463.1988.tb05341.x
出版商:Blackwell Publishing Ltd
年代:1988
数据来源: WILEY
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83. |
An experimental model system for leishmaniasis |
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APMIS,
Volume 96,
Issue 1‐6,
1988,
Page 543-551
KISIA ABOK,
ENRIQUE CADENAS,
ULF BRUNK,
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摘要:
In order to facilitate studies on the effects of chemotherapeutic agents on the host‐parasite interactions in leishmaniasis, we have developed an experimental model for infecting human monocyte‐derived‐ and mouse peritoneal macrophages in culture with recently‐isolatedLeishmania donovanipromastigots (LDP). The chemotherapeutic agents studied were protoporphyrin, hematoporphyrin, menadione, and combinations of hematoporphyrin plus menadione. Since theLeishmania donovaniamastigotes survived poorly in mouse macrophages and protoporphyrin was quite toxic to the latter, our investigations were focused on the effects of hematoporphyrin and menadione on amastigotes engulfed by human macrophages. Treatment ofLeishmaia donovaniamastigotes‐infested human macrophages with either 50 μM hematoporphyrin or 10 μM menadione did not influence significantly the survival of eitherLeishmania donovaniamastigotes or the macrophages themselves. Larger individual doses of hematoporphyrin and menadione were toxic to both parasites and macrophages. The combination of 50 μM hematoporphyrin and 10 μM menadione, however, caused the destruction of the parasites without affecting the host macrophage. The enhanced deleterious effect from combined low doses of hematoporphyrin and menadione is discussed in terms of the production of reactive oxygen species, such as superoxide anion radical and hydrogen peroxide, originating from cellular redox cycling of menadione, and followed by decompostion of the formed hydrogen peroxide by hematoporphyrin to produce the more reactive hyd
ISSN:0903-4641
DOI:10.1111/j.1699-0463.1988.tb05342.x
出版商:Blackwell Publishing Ltd
年代:1988
数据来源: WILEY
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84. |
Thomsen‐Friedenreich‐related antigen in human endometrium |
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APMIS,
Volume 96,
Issue 1‐6,
1988,
Page 552-558
VIBEKE RAVN,
HELLE JENSEN,
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摘要:
The Thomsen‐Friedenreich antigen (T‐antigen) Ga1β1‐3Ga1NAc, is a cryptic disaccharide structure on human erythrocytes. It is masked by sialic acid and uncovered by sialidases as neuraminidase. T‐antigen is a tumor‐associated antigen in epithelial tissues. This study reports preliminary immunohistochemical findings on the expression of the blood group related T‐antigen in human endometrium. Dewaxed sections of 10 proliferative‐, 6 interval‐, 14 secretory endometrium, and 16 endometrial carcinomas, either untreated or pretreated with neuraminidase, were subjected to an indirect immunoperoxidase technique using the monoclonal antibody 49H.8. The monoclonal antibody 49H.8 did not bind to normal cyclic endometrium, unless pretreated with neuraminidase. Neuraminidase treatment revealed staining of apical membranes of glandular‐ and surface epithelium in early or manifest secretory endometrium only. Thirteen of sixteen carcinomas stained without neuraminidase treatment, while all carcinomas stained after pretreatment with neuraminidase. Staining was more widespread than in untreated carcinomas, resembling that of secretory endometrium. Our results suggest: 1) That the sialylated 49H.8 antigen seems to be a marker of cellular differentation in the endometrium, 2) that the antigen defined by the monoclonal antibody 49H.8 might be a tumor‐associated antigen in endometri
ISSN:0903-4641
DOI:10.1111/j.1699-0463.1988.tb05343.x
出版商:Blackwell Publishing Ltd
年代:1988
数据来源: WILEY
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85. |
Campylobacter pyloridetected by indirect immunohistochemical technique |
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APMIS,
Volume 96,
Issue 1‐6,
1988,
Page 559-564
L. P. ANDERSEN,
S. HOLCK,
C. O. POVLSEN,
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摘要:
An immunohistochemical assay for stainingC. pyloriis described. The method is compared with cultivation ofC. pyloriand observation of campylobacter‐like organisms (CLOs) in hematoxyline‐eosine (HE) stained sections. Eighteen biopsies from whichC. pyloriwas cultivated but not seen in HE stained sections and three culture negative biopsies with CLOs seen in HE stained sections were selected from 331 biopsies including 113 culture positive biopsies. There were agreements between cultivation ofC. pyloriand CLOs seen in HE stained sections in the remaining 310 biopsies. Fourteen of the 18 and one of the three biopsies were found positive by the immunohistochemical assay. In addition 21 culture‐positive control biopsies and one of 18 culture‐negative control biopsies were also found positive. When the immunohistochemical assay was compared with cultivation the predictive value of positive result is 93% and of negative result 89%. By this method we were able to detect single organisms and no cross‐reactions to other curved bacteria on the gastric epithelium were
ISSN:0903-4641
DOI:10.1111/j.1699-0463.1988.tb05344.x
出版商:Blackwell Publishing Ltd
年代:1988
数据来源: WILEY
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86. |
Rapid diagnosis ofPneumocystis cariniiusing dark‐field microscopy on bronchoalveolar lavage |
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APMIS,
Volume 96,
Issue 1‐6,
1988,
Page 565-567
Sante Olling,
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摘要:
20 lavages from patients with pulmonary infiltrates were microscopically studied by a rapid dark field assay and after methenamine silver staining. In 8 samples typicalPneumocystis cariniicysts were identified in the methenamine silver‐slides and in 7 of these clusters of round spawn‐like particles were also seen in the dark field microscope. One test was “false negative” in the dark field assay due to presence of too few pneumocystes. Another test was “false positive” due to presence of Candida albicans spores in the lavage. It is thus possible to give aPneumocystis cariniidiagnosis, with some reservation, within 15 minutes after obtaining the bronchoalve
ISSN:0903-4641
DOI:10.1111/j.1699-0463.1988.tb05345.x
出版商:Blackwell Publishing Ltd
年代:1988
数据来源: WILEY
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87. |
In vitrosusceptibility of diarrhoea producing Gram negative enteric bacteria to sulfasalazine, 5‐aminosalicylic acid, sulfapyridine and four quinolones |
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APMIS,
Volume 96,
Issue 1‐6,
1988,
Page 568-570
JAN JESPER ANDREASEN,
LEIF PERCIVAL ANDERSEN,
SUSANNE HARTVIG HARTZEN,
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摘要:
Thein vitrosusceptibility of diarrhoea producing Gram negative enteric bacteria to sulfasalazine, 5‐aminosalicylic acid, sulfapyridine and four quinolones was investigated using an agar dilution method. All strains were resistant to 1600 μg/ml of sulfasalazine and 5‐aminosalicylic acid. MIC range of sulfapyridine forY. enterocoliticawas 3.1–25 μg/ml (median: 6.2) and for Salmonella 25–100 μg/ml (median: 100)Campylobacter jejuni/coliwere less susceptible to sulfapyridine with MIC values ranging from 200 to 800 μg/ml. Shigella and three of fiveE. colistrains were resistant to 1600 μg/ml of sulfapyridine. Two strains ofE. coliwere inhibited by 25 μg/ml. All strains were fairly susceptible to enoxacin, ciprofloxacin, pefloxacin and ofloxacin. Cirpofloxacin was the most active drug on
ISSN:0903-4641
DOI:10.1111/j.1699-0463.1988.tb05346.x
出版商:Blackwell Publishing Ltd
年代:1988
数据来源: WILEY
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