摘要:

The distribution of lysozyme in normal and pathological human gastric and colonic mucosa was studied by light and electron microscopic immunocytochemical techniques and compared with histological and histochemical features. Lysozyme was localized in pyloric glandular epithelial cells, mucous neck cells of fundic glands, Paneth cells and some crypt cells of the mature colonic mucosa. In addition, lysozyme was detected in a large spectrum of “immature” or “regenerative” epithelium: neck cells of the gastric regenerative zone, undifferentiated columnar cells of surface and hyperplastic interfoveolar crests of the stomach, regenerative cells in a healed gastric ulcer, some goblet cells in incomplete intestinal metaplasia, cells of the regenerative zone at the bottom of colonic crypts and, finally, fetal intestinal epithelium. Electron microscopically, we localized lysozyme in the central core of mucous granules in the pyloric gastric glandular epithelium and in the dense mucous granules in gastric mucous neck cells. Lysozyme was also detected in some immature mucin‐producing cells of the gastric regenerative zone and in the rough endoplasmic reticulum of surface hyperplastic columnar gastric cells. At the electron microscopic level, a peculiar correlation between the immunopattern of lysozyme and the morphology of mucous granules has been postulated. All our data support and extend the view that the presence of lysozyme may be related to cell immaturity as well as to a regenerative state of the cell. Finally, the lysozyme distribution and its relation to mucosubstances in gastric and colonic carcinoma suggest that lysozyme should not be considered an exclusive marker of cells of gastric d

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03969.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

We report the first case of disseminated histoplasmosis in an animal in Scandinavia. Yeast cells compatible with those ofHistoplasma capsulatumvar.capsulatumwere found in the skin, liver, spleen, a kidney, and a lymph node of a wild badger (Meles meles). The diagnosis was confirmed by electron microscopy and immunofluorescence staining of the yeast cells in tissue sections.

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03970.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Adrenergic activation is known to occur in sepsis and after major surgery or trauma. An elevated serum concentration of adrenaline is followed by lymphocytosis in peripheral blood even in splenectomized patients. The purpose of the present study was to clarify the redistribution of lymphocytes in the tissues during adrenaline infusion. Lymphocytes were isolated from 24 rabbits, labelled with indium‐111‐tropolone and reinjected into the rabbits. The next day the rabbits were anaesthetized. Eight rabbits received 3 μg of adrenaline i.v. followed by 0.2 μg/min, eight received 300 μg of adrenaline i.v. followed by 20 μg/min, while eight received a saline infusion and served as a control group. The activity of labelled cells was imaged with a gamma camera and computer before, during and after adrenaline infusion. The activity of the spleen decreased to 90% and 94% of initial values during low and high doses of adrenaline. The activity of the bone marrow decreased to 91% and 96%, respectively, while the activity of the heart/lung and the liver increased to 107% and 106% with the high dose of adrenaline. In peripheral blood the lymphocytes increased 10%. It is concluded that lymphocytes are redistributed from spleen and bone marrow to peripheral blood, lungs and liver during adrenaline infusion in this anima

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03971.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Class‐specific IgG and IgA antibodies to HSV were assayed in women with CIN (76), invasive cancer (52) (histological diagnosis) and age‐matched controls (119), employing HSV‐2‐infected HEp‐2 cells as antigen during IFA assay. We observed an elevated geometric mean titre (GMT) of serum antibody (IgG five‐to eight‐fold and IgA four‐to five‐fold) for the entire spectrum of cervical lesions, as compared to controls. The odds of finding HSV‐IgA antibodies were highest with CIN III (OR = 22.0), followed by invasive carcinoma, and CIN I&II (OR = 9.5 and 5.2), respectively. Furthermore, the investigations with respect to married couples (husbands and wives) who volunteered to participate in this study (33 cases and 47 control group) also indicated relatively high antibody titres and increased frequency of HSV sero positivity amongst husbands of cases as compared to their wives, as well as the control group males and females. The contribution of HSV infection in women and/or their husbands to the risk of developing abnormal cervical lesions was analysed after adjusting for the same in respective counterparts. It was observed that the risk was increased 14‐fold with HSV‐IgA positivity of women, and that HSV‐IgA positivity of husbands (male partners) further increased the risk 16‐fold. This preliminary observation shows the importance of serum HSV‐IgA antibodies as a risk indicator in cervical precancer and cancer lesions in women without a history of recent genital herpes lesions. The serum HSV‐IgA may also be taken as an

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03972.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Necrotizing myocarditis due toAspergillus fumigatuswas a contributory cause of death in a patient with acquired immunodeficiency syndrome and non‐Hodgkin lymphoblastic malignant lymphoma of the Burkitt type. A transient remission of the lymphoma had been obtained by cytostatic treatment.A. fumigatuswas isolated from blood two weeks before death, but myocarditis was not diagnosed until autops

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03973.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

This study reports our experience with methods used at our department from 1981 through 1990 for detection of mycobacteria in blood and bone marrow specimens. Direct inoculation on Lowenstein Jensen media was replaced by Isolator® lysis‐centrifugation followed by inoculation on conventional solid media, and the Bactec 12B and Bactec 13A systems. A total of 3033 specimens were analyzed. A total of 137 mycobacterial isolates were obtained from 42 patients, all HIV‐positive except one. Mycobacteremia caused byM. avium‐intracellulare(83%),M. tuberculosis, M. scrofulaceum and M. kansasiiwas found. Of 680 blood specimens tested by the last three methods, 7.6% were found to be positive by at least one method and revealed recovery rates of 6.8% for the Isolator®‐solid media system, 3.4% for the Isolator®‐12B system and 6.9% for the 13A system (all isolates MOTT). Mean detection times for 21 cultures found positive by all three methods were 23.6, 23.3 and 17.7 days for the Isolator®‐solid media, Isolator®‐12B and 13A systems, respectively, with a significantly shorter detection time for the 13A system. Low degree (<1 cfu/ml) mycobacteremia (MOTT) caused delay in the Isolator®‐solid media and the 13A systems and no detection in the Isolator®‐12B system. Antituberculous therapy significantly prolonged the detection times for MOTT in the 13A system in contr

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03974.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Two sets of monoclonal antibodies (mAbs) probably reacting with two different epitopes in the CH3 domain of the human IgG4 molecule were studied. We observed that the commercially available mAb HP 6011 inhibited the antigen binding of the three mutually inhibitable mAbs, 40‐A2, 41‐E8 and 43‐F11 (40‐series), made by us. However, the 40‐series mAbs, including those with similar affinity such as mAb HP6011, were not able to inhibit mAb HP 6011. When the 40‐series mAbs were preincubated with IgG4, the mAb HP 6011 could partially displace these antibodies. This one‐way inhibition indicates that upon binding mAb HP 6011 changes the antigenic structure of the IgG4 molecule by disrupting the epitope for the 40‐series mAbs. A steric hindrance of this epitope by mAb HP 6011 is more unlikely, since the small Fab fragment of mAb HP 6011 also inhibited the reaction of the

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03975.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

A 2.3 kilobase (kb)Salmonellaprobe, JEO402‐1, and two subfragments, F1214 (1.3 kb) and F1217 (0.8 kb), have been evaluated by colony hybridization using pure cultures ofSalmonellaserovars and non‐salmonella bacteria. JEO402‐1, and its subfragments, F1214 and F1217, hybridized to all of 156 differentSalmonellaserovars tested, while there was no reaction to 112 non‐salmonella strains belonging to 19 genera and 37 species ofEnterobacteriaceae. Together with previously published results, the JEO402‐1 probe has now been shown to detect a total of 396Salmonellastrains belonging to 214 serovars ofSlamonellasubspecies I‐VI. A total of 178 non‐salmonella strains representing 23 genera and 51 species ofEnterobacteriaceaehave all tested negative with JEO402‐1. The hybridization results obtained using a digoxigenin‐labeled probe were similar to those obtained with35S isotopic labeling when complete colony

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03976.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

All 97 strains ofSalmonella typhimuriumisolated from patients at a hospital in Nairobi, Kenya, during 1988‐90 were resistant to tetracycline. The minimum inhibitory concentration (MIC) showed a large distribution range from 1 μg/ml to 128 μg/ml. The strains were heterogeneous with respect to plasmid content, but initially all strains possessed, in addition to other plasmids, a large 60‐, 63‐or 65‐MDa plasmid. The tetracycline resistance genes were characterized using oligonucleotide probes, and 20% of the resistant strains possessed tetracycline type A (tetrA), 6%tetrB, and 4%tetrC genes. Three strains possessed both type A and B tetracycline resistance determinants, which were shown to be located on the large 65‐MDa plasmid. There was no correlation between strains isolated from stools, blood, cerebrospinal or epidural fluids, pus, or urine, with respect to the tetracycline genotypes, MIC values or plas

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03977.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The polymerase chain reaction (PCR) was used to amplify a 209 base‐pair fragment ofMycoplasma pneumoniaeDNA. The amplicon was transferred into a plasmid and a 680 base‐pair piece of foreign DNA was inserted between the two amplimer sites. Plasmid DNA was added to the reaction mixture as an internal control for the polymerase chain reaction. Since the original hybridization target sites were included in this construction, one pair of amplimers could be used to amplify both the target DNA and the internal control DNA. Separation of internal control from target DNA after amplification was easily obtained on agarose gel electrophoresis. For the analysis of clinical samples with the polymerase chain reaction, the addition of internal control DNA allowed monitoring of the overall effectiveness of the amplification in each tube. With this technique approximately one‐third of the tests were shown to be unsatisfactory due to technical errors or contaminating inhibitors. Adequate internal controls are necessary to avoid false‐negative results with the polymerase chain r

ISSN:0903-4641    

DOI:10.1111/j.1699-0463.1992.tb03978.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY