摘要:

N. OZCAN, C. CUNNINGHAM AND W.J. HARRIS. 1996. A gene encoding an enzyme which degrades cellulose (end‐1) was isolated from a library ofFibrobacter succinogenesSD35 DNA fragments and expressed in pUC18. The product ofend‐1showed significant activity against carboxymethylcellulose but relatively minor activity against lichenan, xylan and avicel. The nucleotide sequence indicated a product of 388 amino acids with a molecular mass of 50.2 kDa. This was in agreement with the molecular size estimated by gel electrophoresis. No significant DNA sequence similarity was identified with any published endoglucan

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1996.tb01114.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

P. WIGHTMAN, D.E. QUAIN AND P. G. MEADEN. 1996. Production brewing strains of the yeastSaccharomyces cerevisiaewere analysed by DNA fingerprinting, using a Southern blotting and hybridization procedure and employing the Tyl‐15 transposon as a probe. The ability to differentiate readily between strains was very dependent on the restriction enzyme used to digest the DNA prior to Southern blotting and hybridization; the enzymesEcoRI,PstI andSalI were found to be particularly useful in this respect. The method was applicable to the differentiation of both ale and lager yeasts, and was sufficiently sensitive to distinguish between very closely related strains. DNA fingerprinting by this approach confirmed, for example, that a flocculent strain isolated during a production‐scale fermentation with a lager yeast was genotypically different from the par

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1996.tb01115.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY