摘要:

Cellulosomes are cell surface protuberances which contain cellulases functional in substrate adherence and hydrolysis. The mycelia ofThermomonospora curvata, which adhere to and grow on native cellulose fibres, formed cellulosomal structures during cellulase induction, but did not when cellulase biosynthesis was repressed. Cell‐bound enzyme accounted for about 5% of total culture cellulase activit

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01215.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The use of oxolinic acid as a selective agent for the isolation of non‐sporing anaerobes from clinical material was investigated. At a concentration of 5 mg/l it compared favourably with nalidixic acid for this purpose and had the advantage of inhibiting the growth of staphylococc

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01216.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Streptococcus mutanswas cultivated in media containing sucrose (10–40%, w/v) and the sucrose induced changes in chemical and physical properties of its membrane lipids were investigated. The degree of unsaturation in the fatty acids of both total lipid and glycolipid fractions decreased when the sucrose concentration was increased. An electron spin resonance spectroscopic study revealed the reduction of membrane lipid fluidity by adding sucrose to the growth medium. Liposomes prepared from membrane lipids of bacteria grown with sucrose showed less osmotic volume changes than those of bacteria grown without sucrose. These results suggest that modification of membrane lipid composition, fluidity and osmosis‐resistance have an important role in the ability ofStreptococcus mutansto grow in sucrose at high concentrati

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01217.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Naphthalene utilizing bacteria were isolated from several sites above and below the discharge from a coking plant. The distribution of the bacteria was influenced by the effluent. Of these isolates, 11·4% obtained from the effluent discharge site, contained plasmids. No conjugal transfer of naphthalene utilizing ability was observed in over 1000 matings. Curing and transformation experiments demonstrated that one plasmid pNB33 (101 kb) was concerned with naphthalene catabolism

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01218.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A rapid method for measuring thein vitroattachment ofCandida albicansto the surface of transparent acrylic is described. The method involves use of the ‘Magiscan’ automated image analysis system which measures attachment in terms of percentage area coverage. These measurements correlate highly significantly (P<0·001) with the number of adherent yeast c

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01219.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Viablue can be used as a differential viability stain for yeast cells. Differential staining using fluorescence microscopy and viability showed a good correlation (r= 0·99). For practical purposes the staining procedure takes only a few minutes and appears ideally suited to such rapid analytical procedures as the direct epifluorescent filter technique (DEFT) and automated quality control testing of foods and beverages

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01220.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Impedance detection times were compared with traditional plating methods for enumerating antibiotic‐resistant strains ofSalmonella stanley, Salm. thompsonandSalm. infantisgrown in laboratory medium and pork slurry. The correleation of log10counts of salmonellas with detection times was highly significant (r= ‐0·96 for broth andr= ‐0·94 for slurries. The confidence limits (± og101·0 for broth and ± log101·65 for slurry) indicated that detection times could reliably be used as a rapid means of enumerating salmonellas when large numbers of counts of known strains are required for growth studies. Use of antibiotic‐resistant strains also permitted their selective detection by impedance from the natural spoilage flora of pork slurry when the same antibiotics were incorporated in the det

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01221.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The effect of pH value on viability and transduction frequency of thePseudomonas aeruginosaphage F116 was studied. Plaques were formed at pH 5·0–10·0 and transduction occurred at pH 5·0–8·0. Outside the range pH 4·0–11·0 phages rapidly lost viability, but retained the capacity t

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01222.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY