|
|
| 1. |
Factors contributing to the survival of a strain ofPseudomonas cepaciain chlorhexidine solutions |
| |
Letters in Applied Microbiology,
Volume 2,
Issue 3,
1986,
Page 37-42
W. B. Hugo,
L. J. Pallent,
D. J.W. Grant,
S. P. Denyer,
A. Davies,
Preview
|
PDF (323KB)
|
|
摘要:
Adsorption ofPseudomonas cepaciaonto the surface of glass containers enables cells to survive in the presence of user strength chlorhexidine (0.05% w/v). The organisms were found to be firmly attached to the glass and colonised glass slides could be used to infect uncontaminated chlorhexidine solutions. The efficacy of chlorhexidine againstPs. cepaciadecreased with decreasing pH. Storing infected solutions at low temperatures reduced the toxicity of chlorhexidine and promoted longer survival.
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1986.tb01511.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
|
| 2. |
A note on the use of a scanning laser densitometer to analyse silver‐stained lipopolysaccharide patterns on polyacrylamide gels and antigenic profiles on nitrocellulose sheets produced by immunoblotting |
| |
Letters in Applied Microbiology,
Volume 2,
Issue 3,
1986,
Page 43-46
R. K. Tilling,
Helen L. Jessop,
P. A. Lambert,
Preview
|
PDF (312KB)
|
|
摘要:
Silver‐stained patterns of lipopolysaccharide on sodium dodecyl sulphate polyacrylamide gels and microbial antigenic profiles on nitrocellulose sheets produced by electroblotting and immunodetection were analysed with a laser scanning densitometer. Because the patterns fade rapidly it is usual to photograph them immediately to obtain optimum resolution. It has been shown that because of its high resolution the laser densitometer can be used to scan 35 mm photographic negatives without appreciable loss of detail. The method might be of value with whole cell immunoblot antigen profiles as characteristic fingerprints, for example, in taxonomic studie
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1986.tb01512.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
|
| 3. |
The use of agarose wedge‐gel electrophoresis for resolving both small and large naturally occurring plasmids |
| |
Letters in Applied Microbiology,
Volume 2,
Issue 3,
1986,
Page 47-51
P. A. Rochelle,
M. J. Day,
J. C. Fry,
Preview
|
PDF (265KB)
|
|
摘要:
A wedge‐shaped, horizontal agarose gel gave better electrophoretic resolution of both large and small plasmids than conventional linear gels. The wedge‐gel increased mobility and separation of the larger plasmids whilst retarding the mobility of the smaller plasmids and tightening the bands. The linearity of the relationship between log10molecular size and log10relative mobility, for a range of plasmids from 2.1 to 221 kb, was increased. Therefore, estimations of plasmid sizes are more accurate using a wedge‐gel than with conventional
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1986.tb01513.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
|
| 4. |
Evaluation of an enzyme immunoassay technique for the detection of salmonellas in minced meat |
| |
Letters in Applied Microbiology,
Volume 2,
Issue 3,
1986,
Page 53-56
H. J. Beckers,
P. D. Tips,
E. Delfgou‐van Asch,
R. Peters,
Preview
|
PDF (253KB)
|
|
摘要:
A comparison was made between a commercially available enzyme immunoassay (ELISA) and various cultural procedures for detecting salmonellas in minced meat contaminated with a standard inoculum. To detect salmonellas by the ELISA technique, it was necessary to modify the recommended baseline for spectrophotometric measurements to avoid false‐positive results. The incidence of false‐negatives was no greater than that obtained with a standard isolation procedure. Both methods were affected by the competing microfl
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1986.tb01514.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
|
|
首页
