摘要:

When metabolizing aquatic bacteria were determined by the conventional approach to the yeast extract‐nalidixic acid method the apparent percentage of viable bacteria depended on the critical length beyond which cells were scored as viable. An alternative approach allowed the identification of an optimum critical length and the fixing of a lower limit to the true percentage of viable bacteri

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00229.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Attempts to control foam production in exopolysaccharide synthesising cultures ofAcremonium persicinumin aerobic fermentation with polypropylene glycol (PPG) 2025 led to a marked reduction in yield. Similar effects were seen with polysaccharide producing cultures ofEpicoccum purpurascens, but not withAureobasidium pullulans.The effects of other chemical antifoams on polysaccharide production inA. persicinumwere examined, and no inhibition was noticed with any silicone‐based compoun

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00230.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A new medium has been developed for the isolation ofListeria monocytogenesfrom clinical specimens with a mixed flora. Almost complete inhibition of unwanted organisms was achieved and recognition of colonies ofListeriaspp. was usually possible after 24 h using the aesculin‐ferric ammonium citrate indicator system. Compared to McBride agar the new medium was more inhibitory to representative contaminating species in pure culture and more successful in isolating small numbers ofL. monocytogenesfrom artificially seeded clinical specimen

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00231.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The conditions for optimum protoplast formation fromPenicillium nalgiovensewere determined. Best results were achieved using Novozyme 234 in combination with β‐glucuronidase. Chitinase or β‐glucuronidase alone were not able to produce protoplasts under the given conditions. For the regeneration frequency the kind of osmotic stabilizer was important. The use of ionic stabilizers enhanced the regeneration efficiency up to 80% in contrast to only 30% regeneration with sor

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00232.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Buffered solutions of chlorine‐releasing agents, sodium hypochlorite (NaOCl), sodium dichloroisocyanurate (NaDCC), and chloramine‐T, showed similar activity against vegetative cells ofBacillus subtilisbut there was considerable variation in activity against spores, NaOCl showing higher activity than NaDCC, which in turn was more active than chloramine‐T. The effect of coat and cortex extraction on sporicidal activity was determined. It was concluded that although spore coats play a role, they do not totally account for chlorine resistance and that the cortex is also involved, probably through its function in maintaining a low water level in the enclosed core. Observed differences in the sporicidal action of NaOCl, NaDCC, and chloramine‐T may be related to their differing ability to produce core and cortex degr

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00233.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

d‐Galacturonandigalacturonohydrolase was immobilized by covalent coupling on to a polyacrylamide‐type carrier BIO Gel CM100, activated by water‐soluble carbodiimide. Catalytic properties, stability and action pattern of the immobilized enzyme are rep

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00234.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Sediment and the overlying water samples were collected from 67 fresh and 38 salt water sites in the UK. Only 13% (five) of the samples taken from salt water sites were negative for magnetotactic bacteria compared with 47·7% (32) of the fresh water ones. Of the seven types of salt water habitats examined, saltmarsh ponds were found to be most suited to magnetotactic bacteria, 96% (25/26) of sites being positive

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00235.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Strains ofPseudomonasand ofBacillus megaterium, originally isolated from soil by their ability to cleave the carbon‐phosphorus bond of the phosphonate herbicide glyphosate, were not only resistant to the broad‐spectrum phosphonate antibiotics alafosfalin and fosfomycin at concentrations in excess of 2 mmol/1 but could also utilize each as sole phosphorus source. The extent to which their resistance is dependent upon antibiotic detoxification through C‐P lyase activity is un

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00236.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Two hundred and twelve strains representing 11 species of fluorescent pseudomonads along with an additional 198 strains isolated from the leaf and root surfaces of plants were tested for rutin glycosidase, on a mineral base medium supplemented with rutin and glucose. AH of the arginine dihydrolase negative phytopathogens (182 strains) with the exception of 12 of 12 strains ofPseudomonas syringaepv.glycinaand one of 28 strains ofP. syringaepv.phaseoticolashowed rutin glycosidase activity whereas few of the arginine dihydrolase positive strains (22 of 228) were positive.

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00237.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY