摘要:

Proteins in the peribacteroid space (PBS) between the bacteroid outer membrane and the peribacteroid membrane in root nodules ofPisum sativumandVicia fabainduced byRhizobium leguminosarumPRE were analysed by two‐dimensional (2‐D) gel electrophoresis. Most of the detectable proteins were found to migrate to identical positions; however the level of accumulation of some of these appear to be determined by the host plant. When a differentR. leguminosarumstrain (RB1) was used to inoculateP. sativum, the majority of the isolated PBS proteins were found to migrate in the 2‐D gel to identical positions as those of the other two combinations (R. leguminosarumPRE xP. sativumandR. leguminosarumPRE xV.

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01305.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Genes expressingβ‐glucanase activity have been subcloned using the shuttle vector pSA3. Expression has been obtained usingClostridium thermocellum, Ruminococcus albus, andButyrivibrio fibrisolvensgenes inEnterococcus facaelisand by theC. thermocellumgene inBacillus subtilis. Some subclones proved lethal whenβ‐glucanase activity was expressed in conjunction with tetracycline resistance inEscherichia

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01306.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Reproducibility of the Biken test for detection of the LT‐enterotoxin ofEscherichia coliwas evaluated at various concentrations of lincomycin and polymyxin B sulphate. Since 14 out of 65 clinical isolates could not grow at the recommended concentration (90μg/ml) of lincomycin, the standard test was modified by reducing its concentration from 90μg/ml to 60μg/ml and by reducing the concentration of polymyxin B from 500 IU to 30

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01307.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Electron donor‐limited chemostat enrichments were used to isolate sulphate‐reducing bacteria (SRB) from human faeces. When acetate, lactate, propionate, butyrate or amino acids were used as electron donors, SRB identical to those found using traditional isolational methods (agar shake dilution series) were obtained. However, chemostat enrichments facilitated the isolation of SRB able to metabolize mixtures of alcohols (C1‐C5) and mixtures of fatty acids (C2‐C6) which had not been detected by the direct isolation tec

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01308.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Carbon dioxide (30 mmol/l) was shown to inhibit the growth of four strains ofYersinia enterocoliticagrown separately in a simulated milk medium at 7°C and 60 rev/min for 4 d. This indicates that addition of CO2to refrigerated raw milk supplies is likely to be a safe process with respect to that organism

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01309.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Ultraviolet light irradiation of bacterial spores in the presence of hydrogen peroxide has been shown to produce synergistic kills when compared with ultraviolet light (u.v.) and hydrogen peroxide used sequentially. This use in combination has been patented for the commercial sterilization of packaging before filling with UHT‐processed products. Previous results have shown that lamps producing u.v. light with a maximum output at about 254 nm were extremely effective. Results obtained using a Synchrotron radiation source to produce a narrow band of irradiation now shows that the greatest kill of spores ofBacillus subtilisin the presence of hydrogen peroxide is obtained with radiation at ˜270 nm. Such results suggest that the action of the u.v. light is not directly on the spore DNA but may be related to the production of free hydroxyl radicals from hydrogen peroxi

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01310.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Poultry processing plant equipment was sampled forEscherichia colieither sequentially during single processing runs or once weekly for 6 weeks. The biotyping of the isolations revealed a heterogeneousE. colipopulation. The majority of biotypes were identified on one occasion only and never on more than three occasions under both sampling regimes. Although a proportion of the biotypes were recovered from equipment after cleaning, it was concluded that thorough cleaning and disinfection procedures should controlE. colicontamination of slaughterhouse equipment.

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01311.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Protein profiles of whole cells ofBacteriodes ureolyticusgrown in the presence or absence of the iron chelator desferrioxamine mesylate (Desferal) were compared. Each of four strains produced novel proteins of molecular weights 19, 25 and 41 kilodaltons (kDa) under conditions of reduced iron availability. Novel proteins of molecular weights 32, 52 and 58 kDa were also detected although there was interstrain variation in their expression. Outer membranes from three of the strains grown on iron‐depleted medium also contained novel proteins with molecular weights of approximately 25, 41 and 52 kDa. When organisms were grown on medium containing Desferal saturated with excess iron, the novel proteins were not detected indicating that their expression was regulated by the level of available iron in the mediu

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01312.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY