ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00254.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Extracts of black tea, green tea, pu‐erh tea or coffee inhibited the growth of various bacteria known to cause diarrhoeal diseases. Tea or coffee also showed bactericidal activity againstStaphylococcus aureusandVibrio parahaemolyticu

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00255.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

DNA was extracted and purified from 11 strains ofMycobacterium bovisisolated from cattle in Ireland. After digestion with restriction endonuclease PvuII and electrophoresis on an agarose gel, the separated DNA fragments were transferred to a nylon membrane and sequentially hybridized with three DNA probes derived from BCG.None of the three probes detected restriction fragment length polymorphism (RFLP) within the 11M. bovisstrains, indicating a very close genetic relationship. One probe, pBCG12, detected RFLPs between theM. bovisstrains and a reference PvuII digest of DNA fromM. tuberculosisR37Rv, confirming thatM. bovisandM. tuberculosisare closely related though genetically distinct.

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00256.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

No difference in survival was observed when u.v.‐irradiated clostridial cells were subsequently incubated in the dark or exposed to photoreactivating light. This suggests that photoreactivation does not occur inClostridium butyricumand inClostridium acetohutylicu

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00257.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The incorporation of tritiated ([3H]‐) thymidine into DNA by axenic laboratory cultures of a common marine blue‐green algae and two marine eukaryotic algae was measured. Tritiated thymidine was incorporated into the cold TCA fraction of the cyanobacterium and eukaryote algae. However, only in the culture of cyanobacterium was the thymidine consistently incorporated into DNA. Considering the usual algal densities in natural habitats, thymidine concentrations and incubation times, our data do not preclude the use of thymidine incorporation in bacterial production studies in marine environme

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00258.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A new method for detecting salmonellas in foods within 42 h is described. This highly specific and sensitive selective motility procedure gave an efficiency of 96.8% when tested against traditional methods using more than 800 food samples. It is stable at ambient temperatures and can be prepared for use in 5 min.

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00259.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

γ‐Glutamyl‐l‐histidine was synthesized froml‐glutamine andl‐histidine by γ‐glutamyltranspeptidase (EC 2.3.2.2) fromEscherichia coliK‐12. The optimum conditions for the production of γ‐glutamyl‐l‐histidine are determined. The yield of γ‐glutamyl‐l‐histidine synthesized under the optimum conditions was 48% with both substrates (41.2 g/l). The product was isolated and then identified by an amino acid analyser,PMRspectrum and seconda

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00260.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Ewes' milk lactoperoxidase levels in the range of 0.14–2.38 units/ml were detected, with significant differences between individuals, herds and weeks of sampling. Thiocyanate concentration ranged from 0.4 to 20.6 ppm, varying significantly between individuals and weeks of samplin

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00261.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A method is described for the rapid isolation and purification of bacterial genomic DNA. A total of 215 bacterial strains representing species ofCampylobacter, Corynebacterium, Escherichia, Legionella, Neisseria, StaphylococcusandStreptococcus, were lysed with guanidium thiocyanate. DNA was prepared using just three other reagents and one high‐speed centrifugation step. The method, which was applicable to both Gram‐positive and Gram‐negative bacteria, eliminated endogenous nuclease activity and avoided the need for phenol, RNase and protease treatments. The DNA was of high purity, high molecular mass and double‐s

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00262.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Book reviewed in this article:IndustrialMicrobiologicalTesting(1987). Edited by J.W. Hopton&E.G. Hill.DrinkingWaterMicrobiology(1987). Edited by D.O. Cliver&R.A. Newman.Survival andDormancy ofMicroorganisms(1987). Edited by Y. Henis.BrewingMicrobiology(1987). Edited by F.G. Priest&I. Campbell.ThePathogenesis ofBacterialInfections(1985). Edited by G.G. Jackson&H. Thomas.Advances inMicrobialPhysiologyVolume 28 (1986). Edited by A.M. Rose&D.W. Tempest.Advances inMicrobialPhysiologyVolume 29 (1988). Edited by A.M. Rose&D.W. Tempest.MicrobialQualityAssurance inPharmaceuticalsCosmetics andToiletries(1988). Edited by S.F. Bloomfield, R. Baird, R.E. Leak&R. Leech.

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1989.tb00263.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY