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| 1. |
A visual DNA probe for detection of enterotoxigenicEscherichia coliby colony hybridization |
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Letters in Applied Microbiology,
Volume 5,
Issue 5,
1987,
Page 87-90
T. L. ROMICK,
J. A. LINDSAY,
F. F. BUSTA,
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摘要:
A colony hybridization method for visual detection of heat‐labile enterotoxin (LT)‐producingEscherichia colihas been developed. The interchangeability of probe DNA in the system offers the advantage of a more definitive indicator and detection procedure over current plating procedures. Preliminary studies have demonstrated complete specificity of the visual probe for target DNA. This model system has potential for use as a more definitive indicator method for the examination of foods and wastewater for LT toxin ge
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1987.tb01621.x
出版商:Blackwell Publishing Ltd
年代:1987
数据来源: WILEY
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| 2. |
Hydrophobic properties ofProvidencia stuartiiand other Gram‐negative bacteria measured by hydrophobic interaction chromatography |
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Letters in Applied Microbiology,
Volume 5,
Issue 5,
1987,
Page 91-95
N. ISMAEEL,
J. R. FURR,
W. J. PUGH,
A. D. RUSSELL,
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PDF (260KB)
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摘要:
A hydrophobic interaction chromatography (HIC) procedure was used to compare the relative surface hydrophobicity of threeProvidencia stuartiistrains and wild type and envelope mutants ofEscherichia coliandPseudomonas aeruginosa. Providencia stuartiistrain Pv2 adsorbed to a greater extent to octyl‐ and phenyl‐Sepharose than did Pv67. The HIC technique showed a significant difference in surface hydrophobicity between wild type and envelope mutant strains, the latter being considerably more hydrophobic. Pre‐treatment of cell suspensions with chlorhexidine produced further changes in the hydrophobic nature of all the strains. Moreover, HIC provides a convenient and rapid alternative means of screening strains for a property potentially associated with adhesiv
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1987.tb01622.x
出版商:Blackwell Publishing Ltd
年代:1987
数据来源: WILEY
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| 3. |
A monoclonal antibody to T‐2 toxin |
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Letters in Applied Microbiology,
Volume 5,
Issue 5,
1987,
Page 97-99
I. A. GOODBRAND,
W. H. STIMSON,
I. E. SMITH,
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PDF (219KB)
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摘要:
A specific, high affinity monoclonal antibody to T‐2 toxin ofFusariumhas been produced. The monoclonal antibody was conjugated to horse‐radish peroxidase and employed to develop a direct competitive enzyme‐linked immunosorbent assay (ELISA) for the toxin. The sensitivity of the ELISA was 10 ng/ml with a working range up to 500 ng/ml. The antibody cross‐reacted with HT‐2 toxin (25%) but did not bind to any other trichothece
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1987.tb01623.x
出版商:Blackwell Publishing Ltd
年代:1987
数据来源: WILEY
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| 4. |
Adsorption ofThermomonospora curvatacellulases on insoluble substrates |
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Letters in Applied Microbiology,
Volume 5,
Issue 5,
1987,
Page 101-105
B. N. WILLIAMSON,
F. J. STUTZENBERGER,
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摘要:
Cellulosic substrates were tested for their ability to bind the cellulases ofThermomonospora curvata. Protein‐extracted lucerne fibres had the highest adsorptive capacity while native cotton fibres had the least. Analysis of binding as a Langmuir adsorption isotherm yielded maximal binding capacities of 0.012 filter paper units and 0.51 endoglucanase units/mg lucerne fibre at enzyme saturation. This capacity was increased about 12‐fold by fibre pre‐treatment in hot NaOH sol
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1987.tb01624.x
出版商:Blackwell Publishing Ltd
年代:1987
数据来源: WILEY
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