1. |
Evaluation of methods for the enumeration of Campylobacter jejuni and Campylobacter coli bacteriophages |
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Letters in Applied Microbiology,
Volume 10,
Issue 5,
1990,
Page 193-195
S. Salama,
F. J. Bolton,
D. N. Hutchinson,
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摘要:
The Spiral Plater System used for enumeration of bacteria was evaluated for the titration ofCarnpylobacterphages. Twelve phages of C.jejuniwere titrated using the conventional surface droplet technique, soft agar overlay‐pour plate technique, and the Spiral Plater System. Phage counts obtained by the three methods were similar but the Spiral Plater System showed greater precision than the other method
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1990.tb01331.x
出版商:Blackwell Publishing Ltd
年代:1990
数据来源: WILEY
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2. |
Production of β‐glucosidase (cellobiase) byCurvulariasp. |
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Letters in Applied Microbiology,
Volume 10,
Issue 5,
1990,
Page 197-199
U. C. Banerjee,
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摘要:
A Curvularia sp. isolated from soil was found to produce extracellular β‐glucosidase activity when grown in yeast extract, peptone, carboxymethylcellulose (YPC) medium. An initial medium pH of 6·5 and cultivation temperature of 30°C were found to be most suitable for high enzyme productivity. The pH and temperature optima for the enzyme were 4·0 and 70°C, respectively. Under these conditions, the enzyme exhibited a Km(0‐nitrophenyl‐β‐d‐glucoside) value of 0.20 mmol/l. Several divalent metal ions inhibited enzyme activity at high concentration. EDTA. also inhibited β‐gl
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1990.tb01332.x
出版商:Blackwell Publishing Ltd
年代:1990
数据来源: WILEY
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3. |
Identification of a bactericidal component active against Gram‐positive and Gram‐negative bacteria in commercial ovomucoid and ovoinhibitor as lysozyme |
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Letters in Applied Microbiology,
Volume 10,
Issue 5,
1990,
Page 201-204
A. Pellegrini,
K. Grob,
R. von Fellenberg,
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摘要:
During an investigation of the effect of protease inhibitors on the growth of Grampositive and ‐negative bacteria, commercial ovoinhibitor and ovomucoid were found to be strongly bactericidal. Both commercial inhibitors were fractionated by reversed phase chromatography and the bactericidal substance was identified as chicken egg white lysozyme. Lysozyme inhibited the growth of Gram‐negative bacteria without addition of complement or EDTA. The use of ovoinhibitor and ovomucoid must be cautioned by the fact that the commercial preparations are contaminated with lysoz
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1990.tb01333.x
出版商:Blackwell Publishing Ltd
年代:1990
数据来源: WILEY
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4. |
Reduced aflatoxin production byAspergillusparasiticus after growth on a caffeine‐containing medium |
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Letters in Applied Microbiology,
Volume 10,
Issue 5,
1990,
Page 205-207
M. O. Moss,
F. Badii,
M. N. Clifford,
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摘要:
Previous observations that the presence of caffeine could cause a reduction in the specific productivity of aflatoxins byAspergillus parasiticuswere confirmed. However, it was also shown that the reisolation of A.parasiticusfrom media containing caffeine as the sole source of nitrogen, provided a strain with a significantly reduced specific productivity even when grown in the absence of caffeine. This property has remained stable over several subcultures. The specific productivity of this strain is further reduced when it is grown in the presence of sub‐inhibitory concentrations of caffeine. Under normal culture conditions it appears to be indistinguishable from the parent strain except in the reduced specific productivity of aflatoxin
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1990.tb01334.x
出版商:Blackwell Publishing Ltd
年代:1990
数据来源: WILEY
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5. |
An improved method for rapid isolation of plasmid DNA from wild‐type Gram‐negative bacteria for plasmid restriction profile analysis |
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Letters in Applied Microbiology,
Volume 10,
Issue 5,
1990,
Page 209-212
J. E. Olsen,
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摘要:
An improved method for the isolation of large and small plasmids from wild‐type Gram‐negative bacteria has been developed. The protocol combines the lysis and purification procedures of two popular plasmid isolation methods, and produces DNA sufficiently pure for restriction enzyme digestion in less than three ho
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1990.tb01335.x
出版商:Blackwell Publishing Ltd
年代:1990
数据来源: WILEY
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6. |
Antagonistic activity ofRhizobiumspp. against beneficial and plant pathogenic fungi |
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Letters in Applied Microbiology,
Volume 10,
Issue 5,
1990,
Page 213-215
W. ‐L. Chao,
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摘要:
Six differentRhizobiumstrains were tested for their antagonistic activities against 10 isolates of fungi. Their abilities to inhibit the growth of fungal isolates varied tremendously.Rhizobium legurninosnrumbiovarphaseoli6‐3 showed the highest antagonistic activity which significantly reduced the mycelium dried weights of all the fungal isolates tested. This suggests that care should be taken whenRhizobiumand fungal biocontrol agents are to be used togethe
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1990.tb01336.x
出版商:Blackwell Publishing Ltd
年代:1990
数据来源: WILEY
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7. |
Phenolic extract from olives: inhibition of Staphylococcus aureus |
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Letters in Applied Microbiology,
Volume 10,
Issue 5,
1990,
Page 217-220
G. J. E. Nychas,
Soula C. Tassou,
R. G. Board,
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摘要:
The presence of olive extract in different media with or without glucose retarded staphylococcal growth and accentuated the secretion of protein into the media. Moreover the extract influenced the electrophoretic patterns of proteins secretion into the culture supernatant fluid.
ISSN:0266-8254
DOI:10.1111/j.1472-765X.1990.tb01337.x
出版商:Blackwell Publishing Ltd
年代:1990
数据来源: WILEY
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