摘要:

AbstractRecent evidence suggests that anglotens in II may participate in the regulation of Inflammation. We previously reported that anglotens in II in bits its human peripheral blood mononuclear cell reactivity and acts directly on lymphocytes. These observations are again confirmed. In addition, purified OKT8+but not OKT4+T cell suspensions stimulated with phytohemagg uniting revealed Increased thymidine incorporation when simultaneously cultured for 48 hours with angictensin II. These findings suggest that anglotens in II may Inhibit mononuclear cell thymidlne uptake through stimulator of suppresser lymphocytes contained within the OKTE+subpopulation.

ISSN:0892-3973    

DOI:10.3109/08923978609026490

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractIndomethacin (IN) was administered to untreated or to cyclophosphamide (CY) treated C57B1/6 mice to study the roles of prostaglandins in regulating hematopoiesis. The following hematopoietic parameters were quantitated: 1) peripheral blood leukocyte (PBL) count; 2) total nucleated cells per spleen; 3) total nucleated cells per femur; and 4) spleen weight. Assays were performed in vitro to measure the number of colony forming units (CFU) present in the bone marrow and spleen. Untreated mice administered IN had a transient rise in their PBL count. These animals also developed splenomegaly and had an increased number of nucleated cells in their spleen. All CY treated mice had a marked decrease in PBL count, spleen cellularity, bone marrow cellularity, and spleen size during the first 5 days after CY treatment. These observations were followed by hematopoietic recovery over the next 10 days. Cyclophosphamide treated mice exhibited a more rapid hematopoietic recovery when treated with IN than without IN treatment. Analysis of the CFU capacity of bone marrow and spleen cells in soft agar showed a larger number of CFU in the bone marrow and spleen of IN treated mice or of CY/IN treated mice than in animals not receiving IN. These results indicate that prostaglandins are involved in the regulation of hematopoiesis in untreated mice and that prostaglandins may limit the hematopoietic recovery of CY treated mice.

ISSN:0892-3973    

DOI:10.3109/08923978609026491

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractAPSTRACTA lyophilized extract from F. coli (0M-89) was studied for its immunomodulating properties and tolerance in humans. Its oral administration to healthy volunteers produced a selective increase in the active T-cell population without changes in other lymphocyte populations. A significant increase in the proliferative response to concanavalin A and phytohemaaqlutin was recorded, hut not to pokeweed mitogen. No significant changes were observed in the serum levels of IgG, IgA and IgM. The clinical and biological tolerance of OM-89 was excellent, without any adverse side-effects or production of circulating immune complexes or of autoantibodies, while the in vitro investigation showed that it is not a mitogen. Thus in healthy subjects 0M-89 seems to act mainly on the cell-mediated immune responses.

ISSN:0892-3973    

DOI:10.3109/08923978609026492

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractOral administration of diazepam at doses of 5–10 mg/kg to restraint-stressed mice resulted in almost complete recovery in the stress-induced suppression of the antibody response to sheep red blood cell (SRBC). Noreover, this compound restored the suppression of antibody response to SRBC in cyclophosphamide-treated mice. Diazepam treatment also enhanced the antibody response against SRBC in normal mice only when the animals were immunized with the reduced amount of antigen. It was demonstrated that antigen specific helper T cell activity was promoted by diazepam administration in mice. Addition of diazepam augmented the in vitro anti-SRBC hemolytic plaque-forming cell (PFC) response in mouse splenocytes without altering kinetics of the response. However, the enhancing effect was observed only when the drug was added to the medium at the culture initiation. On the other hand, antibody response to T cell-independent antigens such as trinftrophenylated (TNP)-Ficoll and TNP-lipopolysaccharide were not enhanced by diazepam. Concanavalin A or LPS-induced3H-thymidine uptake into splenocytes were not stimulated by diazepam. These results suggest that diazepam promotes the antibody response through stimulating helper T cell functions.

ISSN:0892-3973    

DOI:10.3109/08923978609026493

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractSystemic and local immunological responses of rats sensitized with either M. butyricumor native type II collagen have been evaloatod. In rats exhibiting adjuvant-induced arthritis no antibodies to collagen could be detected. In animals exhibiting collagen-induced arthritis, high antibody titsrs developed by day 14, and could be correlated with the severity of the arthritis.Delayed type hypersensitivity (DTH) responses were measured by a 5-iodo-2′-deoxyuridine 125-I (125-IUdR) uptake assay. Arthritic scores in rats immunized with collagen were not accompanied by a positive DTFt response, where as adjuvant arthritic rats showed a positive response. T-lymphocyte cellular responses in both adjuvant- and collagen-induced arthritic rats were measured. In neither syndrome were major alterations observed in T-lymphocytz subpopulations. These results provide evidence that adjuvant-induced arthritis and type II collagen-induced arthritis are distinct entities, and that they may be discriminated by the nature of the humoral response.

ISSN:0892-3973    

DOI:10.3109/08923978609026494

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractA number of representative flavonoids reversibly inhibit human lymphocyte proliferative responses in a concentration-dependent manner. The flavonoids quercetin and tangeretin are most effective when added during the early phase of exposure of lymphocytes to the mitogenic stimuli but become progressively less effective when added after increasing lengths of time following stimulation, suggesting an early flavonoid-sensitive step(s) in cell activation. In the proliferative response to phytomitogens, they do not act by inhibiting the early increase in calcium influx. They do not augment cellular cyclic-AMP concentration in basal or phytomitogen-stimulated lymphocytes nor reduce its increment in the presence of inhibitors of phosphodiesterase. At concentrations inhibitory to the proliferative response, quercetin (but not tangeretin) inhibits the calcium-activated, phospholipid-dependent protein kinase (C kinase). Certain flavonoids powerfully inhibit the uptake of thymidine into phytomitogen-stimulated lymphocytes but do not directly affect incorporation of already transported thymidine into newly synthesized DNA.

ISSN:0892-3973    

DOI:10.3109/08923978609026495

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractBB rats spontaneously develop an insulin dependent diabetes which resembles in many features human type I diabetes. We have tested the effect of the immunomodulatory drug Ciamexone, a 2-cyan-aziridine-derivative, on the development of diabetes in BB rats. Ciamexone was given once daily during 6 days per week beginning with the age of 42 or 50 days up to 120 days. For comparison cyclosporin A (10 mg/kg) was applied following the same protocol. At 1 mg/kg ciamexone administration led to complete prevention of diabetes in females but was not beneficial in males. At 10 mg/kg the drug caused significant suppression of diabetes development in males but more pronounced in females. Both, a reduction of the incidence of diabetes and a delay in the onset of hyperglycaemia was observed only in females. After administration of cyclosporin A none of the animals developed diabetes.Ciamexone treatment did not affect granulocyte and lymphocyte counts and subsets in the peripheral blood except for a tendency to suppress eosinophilia. The growth of animals was not retarded. It is concluded that ciamexone seems to influence the autoimmune state of the BB rat resulting in partial suppression of the disease.

ISSN:0892-3973    

DOI:10.3109/08923978609026496

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractA soluble form of the reticuloendothelial- and immune modulating agent glucan (glucan-F) has been evaluated for its effects on hanopoiesis. A single 5.0 mg intravenous injection of glucan-F into C3H/HeN mice increased peripheral white blood cellularity, bone marrow and splenic cellularity, bone marrow and splenic granulocyte-macrophage progenitor cell numbers (GM-CFC), and splenic pluripotent stem cell (CFU-s) and erythroid progenitor cell (CFU-e) numbers. Serum levels of granulocytemacrophage colony stimulating activity (CSA) were also elevated following glucan-F administration. These hemopoietic responses correlate well with those previously shown to be induced by intravenous administration of particulate glucan (glucan-P). In contrast to glucan-P, however, intravenous glucan-F administration has been shown not to induce granuloma formation and severe hepatosplenomegaly, thus the potential clinical use of glucan-F as a hemopoietic stimulant is more likely than that of glucan-P.

ISSN:0892-3973    

DOI:10.3109/08923978609026497

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractCyclosprine A (CsA), one compound in the family of cyclosprines, has effectively modulated the course of S-antigen induced experimental autoimmune uveitis (EAU). Cyclosporines G (CsG) and D (CsD), related to CsA in structure, were evaluated in their ability to prevent or modulate EAU in Lewis rats. 10 mg/kg/day IM of CsA effectively prevented the expression of EAU hen therapy began on the day of immunization, while the same dosage of CsG prevented EAU in 81% of animals, and CsD only in 33%. Higher concentrations of CsG (40 mg/kg/day) did effectively block manifestations of the disease. Topical administration of CsG did not prevent the expression of disease but local protection was seen when the 500 ug CsG was placed intracamerally into only one eye. The in vitro comparison of these cyclosprines’capacity to alter proliferation and IL-2 release of a rat T-cell line capable of inducing EAU showed marked differences. CsA appeared to be mst effective at abrogating these cellular functions at all concentrations tested, while CsD was least effective. CsG, however, approached the effectiveness of CsA. CsG is felt to be markedly less nephrotoxic than CsA, the secondary effect that is most commonly encountered, and could potentially be useful in the treatment of human intra-ocular inflamnatory disease.

ISSN:0892-3973    

DOI:10.3109/08923978609026498

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor