摘要:

AbstractSurface markers on rat lymphocytes from thymus and T-cell areas of lymph node, spleen and Peyer's patches were examified in histological sections after one sinale dose of Cyclophosphamide (CY, 100 mg/kg body weight). A panel of monoclonal antbodies was used: W3/13, W3/25 and OX8 to investigate Pan T, THand Ts/cmarker expressions respectively. Ts/cmarker expression showed a marked and significant reduction in both thymus and lymph node lymphocytes 3 days after CY treatment. This was followed by return to normal in the thymus and a significant rebound increase in the lymph node by day 7 after treatment. This TS/cmarker expression in both the spleen and Peyer's patches showed a significant increase on both day 3 and 7 after CY treatment.Mast cells were observed in lerge numbers in the thymus and lymph node but not in the spleen and Peyer's patches. THmarker expression was increased significantly in lymph nodes, spleen and Peyer's patches. No change was observed in Pan T marker expression in any of the tissues at any of the times examined.

ISSN:0892-3973    

DOI:10.3109/08923978609026499

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractThe relationship between enantiomeric homogeneity of three oxazaphosphorine drugs: cyclophosphamide, ifosfamide and trofosfamide and their antitumor activity was evaluated by standard screening tests against four in vivo transplantable tumor models: L 1210 and P 388 lymphoid leukemias, Lewis lung carcinoma and 16/C line of mouse mammary adenocarcinoma. It was shown that the stereodifferentiation of anti-tumor effect of enantiomers was not outstanding although quite consistently in favor of levorotatory forms. The only exception was seen for cyclophosphamide enantiomers tested against leukemias where R/+/ form was more effective than S/-/orracemate.

ISSN:0892-3973    

DOI:10.3109/08923978609026500

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractThis study investigated whether invitroimmunotherapy with rIL-2 which augments human natural cytotoxicity and generation of lymphokine-activated killer cells diminished or inhibits the severity of therapeutically induced human invitroNK immunosuppression. We demonstrate that rIL-2 induces a rapid and potent enhancement of cytolytic killing and that pretreatment of effecter cells for one hour with rIL-2 yields effecter cells which are more resistant to drug-induced immunosuppression. Additionally, we demonstrate cells pretreated for 24 hours with rIL-2 were less sensitive to drug inhibitory effects than rIL-2 non-treated or one hour pretreated effecter cells. Our data suggest prophylactic treatment with IL-2 for drug induced immunosuppression is feasible.

ISSN:0892-3973    

DOI:10.3109/08923978609026501

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractStable activated macrophage hybridomas were generated by somatic cell fusion betweenPropionibacterium acnes-induced peritoneal exudate cells and NS-1 myeloma cells. Five cell lines were obtained and each was cloned by limiting dilution; 59 clones were obtained. The cells of 2 clones (MP4–4 and MP4–8) which adhered to the culture dishes were selected for further analysis. These hybridomas exhibited non-specific esterase and B-galactosidase intracellularly, and asialo GM1, Mac-1, Ia antigens and Fc-receptors on their cell surface. They did not, however, show phagocytic activity or secrete lysozyme. These hybridomas (MP4–4 and MP4–8) secreted the cytotoxic factor without any stimulation. Furthermore strong cytotoxic activity was found in ascites and sera from nude mice inoculated with these hybridomas. These activated macrophage hybridomas should be very useful in studies on cancer immunology and the physiology of macrophages.

ISSN:0892-3973    

DOI:10.3109/08923978609026502

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractResident peritoneal macrophages can be activated to develop cytotoxicity against P815 mastocytoma target cells following incubation in vitro with either D-lactoyl-L-alanyl-γ-D-glutamyl-(L)-meso-diaminopimelyl-(L)-glycine (FK-156), heptanoy1-γ-D-glutamyl-(L)-meso-diaminopimelyl-(D)-alanine (FK-565), or bacterial lipopolysaccharide (LPS) at a minimum concentration of 10µg/ml. Subthreshold levels of hybridoma-derived macrophage activating factor (MAF) markedly potentiated this activity. In an experimental metastasis model, subcutaneous or intraperitoneal treatment with FK-565 (1 to 10 mg/kg) markedly inhibited lung metastasis formation when administered 2–4 days prior to i.v. tumor inoculation. Moreover, this protective activity could be abrogated by the selective macrophage inhibitor, 2-chloroadenosine, suggesting that activated macrophage were responsible for the antimetastatic activity of FK-565.

ISSN:0892-3973    

DOI:10.3109/08923978609026503

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractComplement components and complement. breakdown products have been found to participate in the regulation of the immune response. In the present study we investigated the effect of C3 and its fragments, C3b, C3c and C3d on human allogeneic cell mediated lympholysis (CML). C3 and C3b at a concentration of 275 M×10−9and C3d at a concentration of 330 M×10−9enhanced human allogeneic CML by at least two fold. In contrast C3C did not affect CML responses. Both C3b and C3d had to be present at the initiation of the cultures in order to exert their effect. Similar doses of C3b and C3d did not affect the mixed lymphocyte responses (3H-thymidine uptake) while higher doses were clearly inhibitory. None of the preparations induced proliferative or cytotoxic responses in the absence of allogeneic stimulating cells. C3b and C3d added to the mixed lymphocyte cultures caused increased production of interleukin 2. We conclude that C3b and C3d facilitate allogeneic cytotoxic responses through increased production of interleukin 2.

ISSN:0892-3973    

DOI:10.3109/08923978609026504

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractProf. Dr. med. W. Schaumann dedicated on the occasion of his 60th birthday on November 20, 1986The interaction of T-helper cells and autoreactive B-lymphocytes in the initiation of autoimmune processes is discussed resulting in the hypothesis that this interaction not only leads to the production of autoantibodies but also to the induction of cellular autoimmune processes. Based on this hypothesis, the immunopharmacological properties of a new immunomodulating drug, Ciamexone, are discussed.

ISSN:0892-3973    

DOI:10.3109/08923978609026505

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractAccumulation of lymphocytes after an intraperitoneal (ip) injection of a traditional Chinese herb medicine, XIAO-CHAI-HU-TANG (Japanese name : shosaiko-to), was investigated. Shosaiko-to induced marked accumulation of lymphocytes rather than macrophages in the peritoneal cavity of ICR mice, whereas various kinds of irritants, e.g. proteose-pepton,Escherichia colilipopoly-saccharide (LPS), OK-432 andCorynebacterium parvum, induced preferential accumulation of macrophages rather than lymphocytes. By means of analysis using two-color fluorescence-activated cell sorter (FACS), it was revealed that the increased lymphocyte subpopulations not only in the peritoneal cavity but also in the spleen of C3H/He mice by the injettion of shosaiko-to were comprised of both immature B(IgM+and IgD−) and null (thyl−and Ig−) cells. This effect of shosaiko-to was observed in other C5 normal strains, C3H/HeJ (LPS-nonresponder), C57BL/6, BALB/c and athymicnu/nu(ICR background) mice, but not in C5 deficient strains, AKR/J, A/J and DBA/2 mice, indicating that the accumulation of immature B and null cells in the periphery induced by shosaiko-to is closely related to the complement system.

ISSN:0892-3973    

DOI:10.3109/08923978609026506

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractPeritoneal exudate cells collected from mice 7 days after treatment withBordetella pertussisvaccine exhibited significant in vitro antiviral activity against vesicular stomatitis virus (VSV). Vaccine-induced peritoneal exudate cells exhibited both intrinsic and extrinsic antiviral activity in culture with target VSV-infected L cells. Virus replication was poor in the vaccine-induced exudate cells. Coculture of vaccine-induced exudate cells and VSV-infected L cell targets decreased virus yield. The activity appeared specific for infected cells and at least a portion of the antiviral activity was directed against the initial infection cycle. Nonadherent vaccine-induced exudate cells showed an increase in antiviral activity over total vaccine-induced exudate cells.

ISSN:0892-3973    

DOI:10.3109/08923978609026507

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractWith repeated administration to animals, the cationic, amphiphilic drug, cnlorphentermine (CP), has been shown by others to induce a phospholipidosis in lymphocytes. In the present study mouse splenic lymphocytes, exposed to CP, eitherin vivoorin vitro, developed morphological changes consistant With the induction of phospholipidosis. In addition, CP induced functional changes in lymphocytes. Mice, treated with CPin vivo, demonstrated a significantly depressed ability to generate a delayed hypersensitivity response or to produce antibody-secreting cells againstde novoantigens. Mouse splenic lymphocytes, exposed to 10−7M CP for 3 daysin vitro, demonstrated a signficantly depressed blastogenic response to the mitogens phytohemagglutinin, concanavalin A and lipopolysaccharide.CP inhibited an event that occurred early during lymphocyte activation, but was subsequent to mitogen/receptor coupling. In addition, CP significantly depressed the increased uptake of choline that occurs in lymphocytes following cellular activation. Since the presence of phospholipidosis is indicative of an impairment in phospho lipid metabolism, these results taken together provide evidence for a relationship between this phenomenon and a ltered immune function.

ISSN:0892-3973    

DOI:10.3109/08923978609026508

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor