摘要:

ABSTRACT:To investigate the role of systemic suppression during allopregnancy, CBA/J female mice were immunised against H‐2dprior to mating. Cytotoxic T lymphocyte (CTL) activity was tested at days 14–16 of pregnancy. A reduction of CTL activity was observed only in multiparous animals. Although a nonspecific suppression was detected in isopregnancy, suppression was more marked in allopregnancy. Conversely, the CTL activity observed in the spleen during the first pregnancy (iso or allo) was always significant in mice presensitized with 2 or 3 alloimmunizations prior to pregnancy. Such animals have in vivo effector cells, since allografts of Sarcoma Sal were rejected in secondary fashion in alloimmunized mice, while the fetus remained unharmed. These observations demonstrate that allospecific anti‐MHC CTLs are specifically impaired in multiple allopregnancy, but also tend to rule out theories postulating that systemic suppression of CTL generation and function is required for successful allopreg

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00100.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:Guinea pigs were vaccinated intramammarly (IMM) with attenuated Mycobacterium bovis (BCG) and challenged intramammarly and intradermally with tuberculin. A significant (p<0.05) milk leukocytosis, consisting primarily of polymorphonuclear leukocytes, occurred from 6–30 hr after challenge with tuberculin. Intradermal challenge with tuberculin produced typical delayed‐type hypersensitivity cutaneous reaction in these animals. Normal guinea pigs were adoptively sensitized with lymphoid cells from the inguinal lymph nodes or spleen of BCG‐vaccinated animals and subsequently challenged intramammarly and intradermally with tuberculin. The mammary and dermal responses of the lymphoid cell recipients were similar to, but less pronounced, than those in the actively immunized animals. The responses in the recipients of lymphocytes from donors injected with tuberculin 18 hr prior to cell collection were greater than those from injected donors. Guinea pigs that were injected intraperitoneally with serum from BCG‐vaccinated donors did not express significant dermal or mammary responses to subsequent challenge with tuberculin. It was proposed that the milk leukocytosis was mediated by sensitized lymphocytes stimulated by IMM challenge with tub

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00101.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:The chemical composition of some “immunologically” pure antigens isolated from guinea pig testis and spermatozoa was correlated with their antigenic behavior. Their immunological responses were compared to select the best materials for a further isolation of chemically pure antigens. The glycoprotein extracted from the spermatozoa (T Gly) has the highest immunological potency and seems to be a T and B, depending antigen, able to induce high humoral and cell responses producing germinal cell damage, testicular lesions, and aspermatogene

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00102.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00103.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:One‐way wife‐husband and wife‐donor mixed lymphocyte reactions (MLR) were performed in ten patients with hydatidiform moles and ten age‐ and maturity‐matched pregnant controls. The MLR response in patients with hydatidiform moles was similar to that of normal pregnant subjects and the maternal plasma did not affect the response. In the two patients who subsequently required chemotherapy because of persistent elevation of serum human chorionic gonadotrophin, the maternal plasma taken just before chemotherapy suppressed the maternal MLR response to paternal lymphocytes but not that to unrelated donors' ly

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00104.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:Human seminal plasma has an anticomplement effect that can be measured by a standard immune hemolytic assay. We found that about 20% of samples lack complement‐inhibiting activity. To determine the anatomical origin of a complement inhibitor, inhibition of hemolysis was measured in seminal plasma samples that were primarily of prostatic, vesicular, or epididymal/testicular origin, as well as in seminal plasma from vasectomized men. All samples contained complement‐inhibiting activity, although epididymal fluid showed nearly twice the amount. Therefore, the factor (or factors) is ubiquitous in secretions of the male reproductive tract. Complement‐inhibiting activity is eluted in two high‐molecular‐weight peaks upon gel filtration. We found evidence that the inhibitory factor is not lactoferrin, a proteinase, or one of the proteinase inhibitors known to be present in human seminal plasma. It seems likely that the complement inhibitor in seminal plasma protects gametes and reproductive tissues from complement‐medi

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00105.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:General and specific immune function were examined in women with endometriosis. Nonspecific parameters included total leukocyte and differential counts, quantitative immunoglobulin (IgG, IgA, and IgM) determinations, total hemolytic complement, C3, C4, mitogen‐induced lymphocyte stimulation, and human leukocyte antigen (HLA) typing; no differences were observed when data were compared to age‐matched controls without endometriosis. In contrast, the specific immune response T‐lymphocyte‐mediated cytotoxicity to autologous endometrial cells was significantly reduced (P<0.01) in women with endometriosis. When results from patients were analyzed according to clinical severity of endometriosis, even more striking immunologic alterations were delineated. In addition, lymphocyte stimulation responses to autologous endometrial antigen were lower in patients with severe or moderate disease, approaching statistical significance (P = 0.18 and 0.12, respectively). These studies suggest an immunologic basis for development of endome

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00106.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:Female mice were isoimmunized with homologous spermatozoa of the same strain. Hybrid cells that secrete monoclonal antibodies to mouse sperm isoantigens were generated by modified hybridoma techniques using a semi‐solid Iscove's modified Dulbecco's medium containing methylcellulose for the initial cloning. Out of more than 1,000 colonies that were initially recovered for subculture, 246 were shown to produce antibodies reacting with various cytological regions of mouse spermatozoa, when methanol‐fixed sperm were employed in an indirect immunofluorescent assay. More than 75% of the generated monoclonal isoantibodies were shown to bind the acrosomal regions of mouse spermatozoa. Some were found to cross‐react with spermatozoa from other mammalian species including those of human, rabbit, rat, and guinea pig. However, none were shown to cross‐react with mouse lymphocytes. Two‐thirds of the generated monoclonal antibodies can also bind live mouse spermatozoa. By an immunohistochemical technique using testicular sections, some of these monoclonal antibodies were shown to react with specific antigens expressed during different stages of spermatogenesis. It is concluded that these mouse sperm isoantigens are sperm‐specific and appear uniquely during spermatogenesis. Monoclonal isoantibodies produced in the present study may have potential applications regarding the investigations of sperm iso‐ or autoimmunity, spermatogenesis, and fert

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00107.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The NICHD once again has provided the AJRI with grant summaries of projects in reproductive immunology awarded grant funding for fiscal year 1983. In each issue of Volume 6, we will feature descriptions of some of those grants. The information given in each summary was provided in the grant applicaiton by the principal investigator.The Editorial Board of the AJRI would like to thank the NICHD for its cooperation in regard to this feature of the Reproductive Immunology News section of the Journal It is their hope that continuing to publish this information will continue to facilitate increased communication among investigators in the field of reproductive immunology.

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00108.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00109.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY