ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00163.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00164.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:A cell‐mediated cytotoxicity system was used to characterize the suppressor factors in sera from patients with invasive squamous cell carcinoma of the uterine cervix.The immunoglobulin‐M fraction of the sera of 17 patients with different stages of invasive squamous cell carcinoma of the cervix, separated by Sephadex gel filtration, were tested. All showed marked cytotoxic suppressor activity, including two cases in which sera showed only mild activity.The immunoglobulin‐G fraction of sera from 13 patients with invasive squamous cell carcinoma of the cervix was separated by ion exchange chromatography. The cytotoxic suppressor activity of the immunoglobulin‐G fraction proved to be comparable in effect to that of the whole sera.By using increasing dilutions of IgG and IgM fractions, it could be demonstrated that IgM fraction retained its activity at 0.01 ± 0.005 mg/ml whereas IgG fraction was devoid of activity at this concentration, suggesting that these two immunoglobulin fractions act independently.It is suggested that the cytotoxic suppressor activity in sera from patients with invasive squamous cell carcinoma of the cervix resides in the gamma globulin fraction and that both immunoglobulin‐M and immunoglobulin‐G of their sera manifest the cytotoxic suppressor activity i

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00165.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:51chromium‐labeled syncytiotrophoblasts purified from human placentae were lysed by allogenic leukocytes in vitro. The extent of syncytiotrophoblast lysis was dependent upon the dose of the leukocytes, occurred after a lag period of 42 h, and was inhibited by excess of unlabeled syncytiotrophoblasts. Autologous leukocytes failed to lyse syncytiotrophoblasts. Neuraminidase pretreatment of the syncytiotrophoblasts significantly enhanced their lysis by leukocytes. HCG failed to influence the extent of lysis of untreated syncytiotrophoblasts but significantly reduced the lysis of neuraminidase‐treated targets. The significance of these observations in relation to the “immunologic privilege” of the tropho‐blast in vivo is

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00166.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:The development of immune complexes (IC) and rheumatoid factors (RF) during normal, uncomplicated pregnancy is a controversial issue. Discrepancies in previous reports are due most likely to the different methods used to detect both IC and RF. Using four sensitive radioimmunoassays for immune complexes employing both Clq and mRF and employing sensitive and specific radioimmunoassays for the detection of IgM‐RF and IgG‐RF, we examined the sera of 35 normal subjects in their third trimester of pregnancy. Immune complex concentrations as measured by four assays were not increased during gestation. However, both IgM‐RF and IgG‐RF were significantly elevated even though the concentrations of immunoglobulins M and G were virtually identical to the controls. These observations provide further insight into the immunological changes associated with pr

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00167.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:Thirty‐nine monoclonal antibody (MCA) producing hybridoma cell lines derived from fusions of mouse myeloma cells with spleen cells from mice immunized with human chorionic gonadotropin (hCG) have been established. Their products have been tested in radioimmunoassays using125I‐labeled hCG, luteinizing hormone (LH), follicle‐stimulating hormone (FSH), thyroid‐stimulating hormone (TSH), the alpha (α) and beta (β) subunits of hCG and LH, and the C‐terminal peptide 109–145 (CTP) of CG. All MCA were, in addition, tested in indirect immunofluorescence (IIF) on paraffin sections of human pituitary glands.According to the intramolecular localization of the determinants recognized, three main groups of MCA can be distinguished: 1) MCA directed against epitopes on the β‐chain (α‐MCA), 2) MCA directed against (β‐chain determinants (β‐MCA), and 3) MCA reacting with a conformational determinant only present on the native hormone and not on either subunit (con‐formational‐MCA). All α‐MCA cross‐react with human LH, FSH, and TSH. The β‐MCA do not react with FSH or TSH, but do react to a varying degree with LH. The conformational‐MCA show no binding of labeled FSH or TSH and ver

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00168.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:Acquired immune suppression accompanying normal pregnancy may be associated with reactivation of Epstein‐Barr virus (EBV). Pregnant women with reactivated EBV having anti‐EA antibodies show high titers of antiviral capsid antigen (VCA) geometric mean titers (GMT) of 522 versus 170 in those lacking anti‐early antigen (EA). Among twenty‐seven seropositive women at parturition, 17 (63%) had generated antibody to EA, and all 27 (100%) demonstrated significant increases in antibody to VCA (p<0.01). In contrast, antibody titers to cytomegalovirus, herpes hominis, varicella‐zoster, and rubella viruses in the pregnant women were comparable to those found in nonpregnant

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00169.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:Sera from men at risk for immunity to spermatozoa were screened for antisperm antibodies by immunobead binding following passive antibody transfer to antibody‐free sperm of fertile donors. The percent motile sperm after incubation in diluted antibody positive serum in the presence of complement was compared with the regional distribution of immunoglobulins bound to the sperm surface. The extent of complement‐mediated sperm immobilization varied with immunoglobulin class and with the location of antibody bound to the sperm surface. Tests utilizing complement‐mediated immobilization of sperm are insensitive to the presence of antibodies of IgG and IgA classes that are directed against the head, the distal one‐fifth of the sperm tail principal piece, or the tail end piece. A high degree of immobilization was found only when IgG binding occurred on the distal two‐fifths to three‐fifths of the principal piece of the tail or when IgM bound to the sperm tai

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00170.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:Under in vitro and in vivo conditions, the influence of spermatozoal antibodies on isolated gametes and early embryonic stages up to implantation and into postimplantation fetal development was tested.In the in vitro experiment, a clear‐cut correlation between the amount of antisera added and the reduction of fertilization rates was observed. In the in vivo experiments, female mice were immunized with homologous washed sperms. A correlation between the amount of immunization and the reduction of fertilization rate occurred. In the sperm‐immunized female group, the percentage of large blastocysts showed statistically significant reduction in correlation to the amount of immunization.Spermatozoal antibodies had an effect on isolated gametes, on fertilization, and on the early development of the embryo as well as on implantation.No influence of sperm antibodies either in low or high titer sperm antisera on developmental stages occurred. No spermatozoal antibody‐induced abortions occurred. However, an influence of high titer antisera on implantation was ev

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00171.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00172.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY