摘要:

ABSTRACT:We have confirmed that many women who suffer from recurrent spontaneous abortions have an increased incidence of histocompatibility (HLA‐A, HLA‐B, HLA‐C, and HLA‐DR) antigen‐sharing with their husbands. We also found abortion‐prone women who did not share HLA with their mates; however, they failed to respond or responded suboptimally to their husbands when compared to third‐party controls in the mixed lymphocyte culture (MLC) reactions. Some aborting patients manifested a husband‐specific MLC blocking factor in their plasma which was not present in their serum. In contrast, nonpregnant childbearing wives did not share as many of their husbands' HLA antigens as frequently, had normal or heightened MLC responses to their husbands, and showed no evidence of humoral blocking factors. We propose that HLA typing, MLC reactions, and blocking factor studies will serve as useful laboratory indices in future clinical studies of immunogenetical disorders associated with idiopathic ha

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00272.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:The immunologic responsiveness of eight women who habitually abort has been investigated. All shared an HLA‐A or B antigen with their husbands. Sharing of an HLA‐DR antigen was found in seven couples, one of which also had a second DR antigen in common. The probability for this high frequency of HLA‐DR sharing is negligible (p = 0.0004), as calculated from the antigen frequencies among Europeans. Cells from the woman with two shared DR antigens displayed a minor response to her husband's cells but reacted strongly to control cells, whereas the other women's cells reacted normally to cells from both their husbands and controls in one‐way mixed lymphocyte culture (MLC). Only minor cytotoxicity was displayed by women's cells in a direct cell‐mediated lympholysis (CML) assay, but they mounted normal cytotoxic responses against both husbands' cells and control cells in an amplified CML assay. The sera from six of the habitually aborting women displayed no blocking activity in one‐way MLC, and seven of them had no cytotoxic antibodies. Cells from all habitual aborters were suppressed in two‐way MLC by cells from husbands and most controls. We hypothesize that increases in HLA compatibility between mother and fetus and in maternal susceptibility to suppressive influences are in some way linked to a deficiency in the development of anti‐fetal antibody during pregnancy. As a consequence, the fetus may be deprived of the protection by maternal blocking antibody, which may allow maternal cytotoxic reactions to

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00273.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00274.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00275.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:The molecular configuration of group‐specific component Gc protein in sera from pregnant and nonpregnant individuals was compared by analytical isoelectric focusing and by print immunofixation in conjunction with known standards of Gc:actin and Gc:vitamin D3complexes. These studies revealed that while complexes of Gc with actin and with vitamin D were detectable in small amounts in nonpregnant sera, much larger quantities of both types of complexes were consistently visualized in pregnancy. In addition, when actin was added to pregnant sera containing Gc:vitamin D3complexes, a third anodal complex was revealed which presented the molecular configuration of actin: Gc: vitamin D3. These results demonstrate that Gc:actin complexes may be present under physiological circumstances in the circulation. Since large amounts of trophoblast enter the maternal circulation during both normal and abnormal human pregnancy, experiments were undertaken that showed that actin was released from isolated trophoblast membranes and also upon lysis of other viable cells under physiological conditions similar to those obtained in serum, and such actin complexed rapidly with Gc. Although the effects of this phenomenon upon the immunobiology of pregnancy are unknown, these findings are consistent with the concept that Gc protein may exert a “scavenger” function in mopping up actin released from damaged

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00276.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00277.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00278.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY