摘要:

ABSTRACT:Eighty‐one patients who had a diagnosis of primary ovarian failure were studied to determine its possible association with autoimmune disorders. All 81 patients displayed 46, XX chromosome complements. On ovarian biopsy, either few or no follicles were demonstrated in 79 patients, and, in two patients, primordial follicles were more abundant. The two patients with a large number of primordial follicles had normal function of other endocrine organs; however, 15 of the 79 patients demonstrating few or no ovarian follicles had associated failure of other endocrine glands, and one patient had myasthenia gravis. Thirteen of these 15 patients sought treatment because of secondary amenorrhea, the age at onset ranging from 11 to 34 years. Of the 81 patients, 11 had primary amenorrhea and 70 had secondary amenorrhea. Among the 79 patients with few or no ovarian follicles, endocrine glandular failure, in addition to ovarian failure, was found in two patients with primary amenorrhea and in 13 patients with secondary amenorrhea. The association of polyglandular failure syndrome and primary ovarian failure, along with demonstration of a lymphocytic infiltrate in ovaries and circulating antibodies in sera of women with premature ovarian failure, suggests that an autoimmune mechanism may be a cause of primary ovarian failure in some case

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00254.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:The autoantigens of rat male accessory glands were isolated by a short procedure which involved 1) immunization of rats with chemically modified rat male accessory glands' saline extract; 2) purification of immunoglobulin G (IgG) from the autoantisera by chromatography on DEAE‐Sephadex A‐50; 3) coupling of rat IgG anti‐rat male accessory glands with 4‐B activated Sepharose; 4) addition of rat male accessory glands' saline extract and removal of autoantigens by glycine‐HCl, pH 2.9. The purity of the eluted autoantigens was determined by polyacrylamide disc gel electrophoresis (PAGE). These components retained their immunologic activity as demonstrated by inhibition of tanned cell hemagglutination and double immunodiffusion gel prec

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00255.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:The aim of this report is to investigate the effect of cyclophosphamide (CY) in an experimental model of autoimmunity to rat male accessory glands. The results indicated that 100 mg/kg of this drug suppressed humoral immune response that persisted for at least 45 days when administered 3 days after the first immunization of rats with modified rat male accessory glands (MRAG) in complete Freund's adjuvant (CFA). Administration of the drug 3 days before ID injection of antigen caused a shorter suppression of antibody formation. Delayed type hypersensitivity (DTH) studied 13 days after the first immunization was suppressed only in the animals that were administered CY after the antigen.The specificity of the immunosuppression was studied in rats treated with CY after the first immunization with MRAG using aggregated human γ‐globulin (AHGG) as an unrelated antigen. The studies demonstrated significant suppression of DTH (p<0.005) and humoral immunity only against MRAG. On the contrary, the response to AHGG was not significantly modifi

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00256.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:Immunodiffusion, immunoelectrophoresis, SDS‐polyacrylamide gel electrophoresis, polyacrylamide gel electrophoresis, ultracentrifugation analysis, and gel permeation chromatography in chaotropic and detergent mediums showed that the human seminal plasma obtained and stored in the usual way is mainly composed by heterodispersed glycoproteins of low molecular weight.The glycoprotein components of the human seminal plasma which do not interact specifically with concanavalin A (con A) were separated by column chromatography on Con A‐Sepharose according to nonspecificion exchange and hydrophobic interactions with the protein.The carbohydrate composition of the glycoproteins and the existence of the N‐acetylgalactosaminylserine (or threonine) linkage, as well as their aggregation properties show that they are mucin‐type glycoproteins.They resulted “immunologically pure’ although the carbohydrate chains show the structural heterodispersion usually found in mucins.The glycoproteins have a common structural pattern for the terminal nonreducing, determinant‐bearing sequences oftheir oligosaccharide chains which, together with the low molecular weights and the known fact that mucus secretions of normal cells contain only one or two types of glycoproteins, suggest that they are fragments produced by endogenous proteolytic degradation of a

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00257.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:Maternal immune suppression is a potentially significant adverse effect when betamethasone is used to hasten lung maturation of the fetus at risk for preterm delivery. However, increased incidence of infection has not been observed consistently after betamethasone treatment of pregnant women. This study was designed to determine if the cellular immune response to steroids may be modified during pregnancy in a way that would diminish the infection risk associated with steroid treatment.The effect of betamethasone on immunocytes among patients with preterm labor or in nonpregnant subjects were determined following administration of 12 mg of betamethasone intramuscularly. We measured serially the circulating leukocytes, lymphocytes, T lymphocytes, and their subsets. Measurements were also made of localized leukocyte mobilization to serum‐filled skin chambers covering experimental inflammatory sites. Patients in preterm labor had increased WBC counts prior to treatment with betamethasone but no additional leukocytosis was induced nor was mobilization of leukocytes to the skin chambers decreased. Lymphopenia and depression of T cells was more transient among pregnant patients compared to nonpregnant. Thus, the pregnant patients studied had diminished or more transient potentially adverse immunocyte responses to betamethasone as compared to nonpregnant subject

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00258.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:Many observations suggest that cellular immunity is depressed during gestation. To define alterations in subsets of circulating lymphocytes during pregnancy, we compared the percentages of total T cells, T helper cells, and T suppressor cells in the peripheral blood of 31 pregnant women at various stages of gestation with those in that of nonpregnant controls. Control studies consisted of five women ingesting oral contraceptives and five women menstruating spontaneously. Isolated peripheral blood mononuclear cells were reacted with fluoresceinated monoclonal antibodies that were specific for Leu‐2 cells, Leu‐3 cells, and Leu‐4 cells and that were subsequently analyzed on a fluorescence‐activated cell sorter. No variation in the percentage of Leu‐2, Leu‐3, or Leu‐4 cells was observed during the menstrual cycle or oral contraceptive cycle. No differences were seen when T‐cell subsets at various stages of gestation were compared with the corresponding T‐cell subset in nonpregnant controls. The data suggest that pregnancy does not alter the percentage of T‐cell subsets in t

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00259.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:The capping of lymphocytes from normal pregnant women is diminished. Decreased capping is observed for both anti‐Ig and for Con A receptors. The altered capping response appears between the tenth and the twelfth week of pregnancy. The diminution in capping persists until about 2 weeks after delivery. The possible association between the decreased capping response and the altered immune responses known to occur during pregnancy is discussed. The factors underlying the altered capping response in pregnancy may be related to the factors responsible for the parallel phenomenon in pathological condition

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00260.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:Mouse milk cells were stained with rhodamine or fluorescein isothiocyanate and fed to young suckling mice. By visual examination of serial sections and by flow cytofluorometry, we were able to demonstrate directly the presence of these cells in peripheral tissues.It was estimated that at least 0.1% of the fed cells might infiltrate the young mouse, which is initially immunologically defenseless. This is in accordance with evidence from many sources for activity of maternally‐derived lymphoid cells in young rodent

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00261.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00262.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY