摘要:

SummaryThe performances of two commercial screening tests for glucoses‐phosphate dehydrogenase deficiency (Sigma fluorescent spot test and Sigma colorimetric method) were assessed in order to determine their usefulness in a routine haematology laboratory. As a first step, three ranges for enzyme activity were determined as follows: a ‘normal’ range determined from the 95% confidence limit of assays carried out on 114 normal adult males, a deficient range calculated as 25% of the upper limit of normal or less, and an intermediate range between the lower and upper values of these ranges. These values were 4.9–11.8 u/g Hb, 0–2.9 u/g Hb and 3.0–4.8 u/g Hb respectively. A separate normal range was also determined for females and was 5.5–12.8 u/g Hb. The two screening tests were then assessed against these values and the kits were found to be equally reliable at predicting normals and deficients but less reliable at detecting all female heterozygotes. The criteria for using the different procedures were evaluated. It is concluded that screening procedures are useful only when a large number of tests are routinely performed or in the absence of facilities for assays, as they offer no advantage in cost or time over the assay procedure. The colorimetric test (single vials) is easier and cheaper to use if isolated tests are performed, whereas the fluorescent spot test (with some modifications) is more useful for carrying out numerous tests s

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb01113.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryThe presence of certain antibiotics in patients' sera can invalidate microbiological assays for vitamin B12usingLactobacillus leichmanniiby inhibiting organism growth. Analysis of 3291 consecutive serum vitamin B12assays in a large general hospital service showed inhibition of growth in 303 samples (9.2%). In nearly all cases the interfering substance was a beta‐lactam antibiotic. The value of using a beta‐lactamase preparation to eliminate antibiotic effects was studied using antibiotic‐containing patient sera and also normal serum to which antibiotics were added. In‐vitro additions of penicillins, cephalosporins, and other antibiotics to normal serum resulted in varying levels of organism inhibition, but excellent recovery of a control value after treatment with beta– lactamase (>98%). Patient sera showing inhibition were reassayed in duplicate following treatment with beta‐lactamase (n= 200), the overall effectiveness of the treatment being verified by the excellent recovery (>98%) of a cyanocobalamin ‘spike’ added to a control. In only 0.2% of cases (6 of 3291 samples) was this treatment ineffective and it has thus proved an efficient method of eliminating the problem of antibiotic interference in the vitamin B12microb

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb01114.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb01115.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb01116.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb01117.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb01118.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb01119.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb01121.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Book reviewed in this article:Haematology Revision for MRCP and MRCPathBy A. S. J. Baughan, D. C. Linch, S.J. Machin, K. G. Patterson, L. Tillyer&J. G. Treleaven.Leukaemia Cytochemistry: Principles and PracticeEdited by C. S. Scott.Postgraduate Haematology (third edition)Edited by A.V. Hoffbrand&S.M. Lewis.Clinical HaematologyBy D.L. Barnard, B.A. McVerry&D.R. Norfolk.Clinical Practice of Transfusion MedicineEdited by Lawrence D. Petz&Scott N. Swisher.Fanconi Anaemia: Clinical, Cytogenetic and Experimental AspectsEdited by T.M. Schroeder‐Kurth, A.D. Auerbach&G. Ob

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb01122.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY