摘要:

SummaryHIV associated thrombocytopenia occurs in 5–10% of asymptomatic individuals, and 25–45% of people with AIDS. A variety of treatments are available but the inherited bleeding disorder in addition to the thrombocytopenia makes management in haemophiliacs a special problem. The management of this double coagulopathy is described in seven patients. IV gammaglobulin and steroids were useful only in the short term; zidovudine produced a good response, could be taken orally, had anti‐HIV activity and was well tolerated in asymptomatic HIV positive patients. Interferon was an alternative when zidovudine was ineffective or produced intolerable side‐effects. Splenectomy was considered only after failure of other treatments because of the increased risks of b

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00033.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryThe results of a large number of nutritional screen requests (n= 871) were compared with corresponding values of erythrocyte indices considered predictive of nutritional deficiencies to determine if such indices could be used in a prospective screening procedure to restrict the number of serum vitamin B12, folate, and ferritin assays. Low mean cell haemoglobins (MCH<27 pg) were found to be superior to low mean cell volumes (MCV<77 fl), in predicting low serum ferritin values. The occurrence of deficient ferritin values was 90% when the MCH was very low (MCH<23 pg). Vitamin B12or folate deficiency could not be predicted from the MCV. A normal MCV was found in more than 55% of vitamin B12deficient samples and some 30% of serum B12deficients (12 gm/dl) or macrocytosis (MCV<100 fl). It would not seem appropriate to use erythrocyte indices alone as a method of selecting samples for further investigation of folate or vitamin B12status

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00034.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryIn 83 healthy normotensive males aged 20–55 years the platelet count is positively correlated with the red cell count (r= 0.371;P= 0.0006), the white cell count (r= 0.358;P= 0.0009), and with weight (r= 0.252;P= 0.0269). The red cell count is also positively related with the white cell count (r= 0.242;P= 0.0278) and with weight (r= 0.326;P= 0.0039); while the white cell count is slightly correlated with weight (r= 0.210;P= 0.067). These findings provide further indirect evidential support for a haemopoetic growth factor acting on a single pluripotent stem cel

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00035.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryA 44‐year‐old woman experienced recurrent episodes of massive intravascular haemolysis complicated by acute oliguric renal failure over a period of 22 years. The haemolysis was induced by IgG warm type autoantibody and complement and responded to corticosteroid therapy. The renal failure was treated effectively by dialysis. To our knowledge, such a life long occurrence of recurrent intravascular haemolysis induced by IgG warm type autoantibodies together with renal failure has not been reported bef

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00036.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryNon‐surgical and surgical asplenia predisposes to fatal infections; therefore, simple, non‐invasive and repeatable tests for assessing splenic function are required, even in non‐specialized medical institutions. Howell‐Jolly bodies are the most characteristic peripheral blood abnormality after splenectomy, but their counting is not considered a reliable measure of splenic function. In this study, in a group of splenectomized subjects and of patients with non‐surgical hyposplenism, we have compared counting of Howell‐Jolly bodies, stained by both the May‐Grünwald/Giemsa method and the Feulgen reaction, with pitted cell counting which is considered a reliable technique for the assessment of splenic hypofunction. A significant correlation has been found between Howell‐Jolly body counts, stained by either technique, and pitted cell counts (P<0.0001). Though Howell‐Jolly bodies were never detectable when pitted cell counts fell between 4 and 8%, values consistent with a very mild splenic hypofunction, for pitted cell counts above 8% their increase was always associated with increasing Howell‐Jolly body counts. These data suggest that, although pitted cell counting represents a more sensitive method for evaluating splenic function, Howell‐Jolly body counting may still be regarded as a simple and reliable technique for identifying and monitoring those cases associated with a real risk of o

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00037.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryPlatelet counts on whole blood samples collected into tripotassium salt of EDTA, trisodium citrate (Na3citr), citrate phosphate dextrose adenine formula 1 (CPDA‐1) and acid citrate dextrose formula A (ACD‐A), all showed a statistically significant drop (P<0.01) after 1 h standing at room temperature (RT) as compared with the immediate (within 30 min) counts. After 1 h the enumeration became stable in the EDTA samples but the drop continued up to 4–6 h in those samples taken into citrate. The decreases in citrate were significant (18–30%,P<0.001). The addition of EDTA (1.5 mg/ml) to the citrated samples after the sixth hour count created a significant rise (6–22%,P<0.01) in the counts between the sixth and the seventh hour. Our observations show that platelet counts in citrated blood samples arc lower than those in EDTA and highlight the necessity to present citrated samples mixed with dried EDTA when characterizationor quality control of blood and blood components is required. Analysis of the mean platelet volume (MPV) showed significantly lower values (6–13%,P<0.05) in the citrated samples as compared to the same samples in EDTA, and a significant increase (4–6%,P<0.01) on the addition of EDTA to the citrated samples after the sixth

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00038.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryRecently flow cytometry techniques have been developed to replace the microscope reticulocyte count. We used thiazole orange, a RNA binding fluorochrome, to discriminate reticulocytes from mature erythrocytes. Thiazole orange and the Retic‐COUNTTMsoftware package were evaluated for performance of routine analysis on different flow instruments. The applied methodology analysed 104cells semi‐automatically in an easily performed manner. Consistent results were obtained with dipotassium EDTA anticoagulated blood (stable for 30 h after venesection), with incubation times in thiazole orange solution ranging from 2 to 7 h at 25C. This allowed flexibility in specimen collection and storage and assay performance with no change in results. Changes of incubation temperature up to 30°C had no measureable effect. The values obtained showed good linearity, precision and accuracy for normal, low and high reticulocyte counts. However interferences were observed: RBC autofluorescence, nucleated RBC, Howell‐Jolly bodies, high leucocyte count, high platelet count and giant platelets, all falsely increased the number of reticulocytes. These artifacts were eliminated by software gate corrections, thus leaving less than 5% of the specimen to be reanalysed by the microscopic method. The thiazole orange flow cytometric method was determined to be a fast, reliable method for the routine clinical quantitation of reticul

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00039.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryIn order to study normal and abnormal human megakaryocytopoiesis we modified a glycoprotein lb dependent megakaryocyte purification technique originally described in guinea pigs. After Percoll density centrifugation, 2 ml bone marrow aspirates were subjected to 3 steps of bovine plasma induced cell clumping, which should selectively agglutinate glycoprotein Ib positive cells. In 13 experiments the resulting samples contained 28–102 × 103megakaryocytes (median 70) with a median purity of 79%. Megakaryocyte recovery ranged from 30–86% (median 51%). The DNA profile showed median values of 10% for 8 N, 44% for 16 N and 43% for 32 N megakaryocytes. The described method is relatively simple, inexpensive and gives results comparable with more elaborate techni

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00040.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryObservations on rosette formation of red blood cells sensitized with a known number of IgG1 or IgG3 polyclonal or monoclonal anti‐Rh(D) antibodies with monocytes, lymphocytes and granulocytes have provided further evidence for the essential role of monocytes/macrophages in red cell destruction. The rosette test with monocytes and the monocyte‐monolayer assay (MMA) have confirmed, on a quantitative basis, the greater binding ability of IgG3 than of IgG1 antibodies and that the rate of interaction with red cells increases in proportion to the level of their sensitization. These observations have suggested that the MMA may reflect haemolysis in vivo. It has appeared that the MMA is suitable for assessing haemolysis in many cases of haemolytic disease of the newborn, in autoimmune haemolytic anaemia and in patients with alloantibodies to be transfused. Since in individual cases a clear correlation between the MMA results and haemolysis in vivo was not observed, the factors which may influence such discrepancies are discus

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00041.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1990.tb00042.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY