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1. |
Pilot study: effects of G‐CSF on neutrophilex‐vivofunction post bone marrow transplantation |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 79-85
M. G. MACEY,
J. SANGSTER,
S. M. KELSEY,
A. C. NEWLAND,
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摘要:
SummaryThe effects of human recombinant granulocyte colony stimulating factor (G‐CSF) on neutrophil recovery have been studied in patients undergoing bone marrow transplantation for haematological disease. Nine patients (five autografts and four allografts) were studied while receiving daily doses of G‐CSF (range 2‐20 μg/kg body weight) were compared to eight patients (four autografts and four allografts) who did not receive G‐CSF as a control group. In both groups flow cytometry was used to determine neutrophil IgG Fc receptor (FcRII, FcRIII) expression. Phagocytosis and metabolic burst was assessed using IgG opsonized bacteria. The patients' neutrophils were studied prior to conditioning in autografts and donor cells were studied in allografts. Studies were repeated at neutrophil recovery (neutrophil count 1.0 ± 109/1) and ten days post recovery. At recovery FcRII expression was slightly increased in all groups, whereas the number of cells expressing FcRIII was reduced. This reduction was significant in the patients receiving rG‐CSF at recovery and post recovery compared to the pretreatment levels. The ability to phagocytose bacteria in cells from patients receiving G‐CSF was also reduced. The associated metabolic burst was significantly reduced in the autograft group but not the allograft group receiving rG‐CSF. The reduced function and reduced expression of FCRIII suggests the presence of immature neutrophils in patients receiving
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00130.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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2. |
Suppression of erythroid progenitor cells during malarial infection in Thai adults caused by serum inhibitor |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 87-92
S. JOOTAR,
W. CHAISIRIPOOMKERE,
P. PHOLVICHA,
A. LEELASIRI,
W. PRAYOONWIWAT,
W. MONGKONSVITRAGOON,
T. SRICHAIKUL,
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摘要:
SummaryThe bone marrows of 21 Thai adults infected withPlasmodium falciparummalaria were cultured for CFU‐E and BFU‐E by using AB serum, autologous serum (parasitaemia) and autologous serum (post‐parasitaemia). Six patients had no complication and 15 patients had pulmonary, renal or haematologic complications. In the non‐complicated cases, sera during parasitaemia did not suppress the post‐parasitaema CFU‐E and BFU‐E. Post parasitaemia, there was suppression of CFU‐E by parasitaemia sera. In the complicated cases, the autologous sera during parasitaemia suppressed the growth of both CFU‐E and BFU‐E both during and after parasitaemia (P<0.05). The post‐parasitaemia sera had neither a suppressive nor a stimulating effect. In the complicated cases, the progenitor cells cultured from the bone marrow post‐parasitaemia were fewer in number than those cultured from the bone marrow during parasitaemia using the same sera. Two possible mechanisms of suppression are postulated, namely the reduction of erythropoietin or the increased tumour necrosis factor during malarial infection. Further studies to clarify thi
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00131.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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3. |
Alcohol induced burr cell (echinocytic) haemolytic anaemia and haemochromatosis |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 93-102
N. BIZZARO,
I. PIAZZA,
G. BALDO,
A. BARITUSSIO,
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摘要:
SummaryA 51‐year‐old man with chronic alcoholic liver disease developed a severe haemolytic anaemia characterized by the presence of circulating burrshaped cells (echinocytes). Several transfusions of packed red cells were ineffective in raising the haemoglobin concentration, showing that the abnormality was acquired by the transfused cells. Liver biopsies revealed haemochromatosis. Haematological parameters normalized four months after the patient stopped drinking alcohol, but burr cells were still present and erythrocyte life‐span was still markedly shortened at one year follow‐up. Since serum cholesterol, HDL‐cholesterol, and Apo‐Al and Apo‐B lipoproteins were considerably decreased, the lipid composition of the red cell membrane was studied. Findings showed that echinocytosis occurred with no change in membrane cholesterol content, nor in cholesterol: phospholipid ratio, but with an alteration in the phosphatidylserine and phosphatidylinositol concentrations. While haemochromatosis was most likely the cause of the erythrocyte anomaly, alcohol intake was probably responsible for the acute onset of haemolytic anaemia with effects directly on the erythrocyte membrane as well as mediated by the progressive hepatic injury, with alterations in the plasma and successively in the intramembrane lipi
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00132.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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4. |
Comparative evaluation of three flow cytometers for reticulocyte enumeration |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 103-111
M. PETEGEM,
R. CARTUYVELS,
P. SCHOUWER,
V. DUPPEN,
W. GOOSSENS,
L. HOVE,
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摘要:
SummaryFlow cytometric determination of reticulocytosis is progressively replacing the manual microscopic method. We evaluated three flow cytometers (Becton Dickinson FACScan, Coulter EPICS Profile II, Ortho Cytoron Absolute) for reticulocyte enumeration, using thiazole orange. For each sample, 30000 cells were analysed. In order to comparatively evaluate the three instruments, reticulocytes were counted by manually gating the erythroid population and evaluating the gated population for fluorescence characteristics. The different instruments showed good linearity and precision. No carry‐over was observed. Orthogonal regression analysis of reticulocyte counts of 100 healthy blood donor samples and 108 haematological patient samples showed a good mutual comparability between all three instruments tested, although the pairedt‐test showed a significant difference between the Cytoron and both the FACScan and the Profile. Despite minor statistical differences, the three instruments can be considered equivalent for daily routine reticulocyte enumerat
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00133.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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5. |
Simultaneous analysis of cell surface antigens and DNA content by flow cytometry |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 113-118
D. J. HOWE,
J. A. HOPKINS,
S. A. N. JOHNSON,
M. J. PHILLIPS,
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摘要:
SummaryCells obtained from the blood or bone marrow of patients with haematological malignancies can both be stained with fluorescent labelled monoclonal antibodies to determine an immunophenotype and permeabilized with 30% ethanol then stained with propidium iodide for simultaneous DNA analysis. In the technique described here, no evidence of selective depletion of the malignant cell population was demonstrated and decreases in the mean fluorescence intensity of the surface markers were insufficient to affect the sensitivity of flow cytometric analysis. The combined method is simple and robust enough to allow incorporation of DNA analysis into routine immunophenotyping of leukaemia and lymphoma cells.
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00134.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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6. |
Use of a low pressure liquid chromatography system for haemoglobinopathy screening |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 119-128
K. CHAMBERS,
A. PHILLIPS,
C. S. CHAPMAN,
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摘要:
SummaryA closed low pressure liquid chromatography system (LPLC) is described which is suitable for haemoglobinopathy screening. The system (Glycomat) was originally developed for the quantitation of the glycated haemoglobin (HbA1). The same biochemical principles have been applied to the separation of haemoglobin A2(HbA2) and haemoglobin variants. The instrument offers three modes of use including a fast haemoglobin elution, a variant screen and a HbA2assay for thalassaemia screening. The fast screen isolates all of the common haemoglobin variants except HbE which elutes with HbA. This mode is a more rapid alternative to the Sickledex test. The variant screen produces a wider separation of abnormal variants giving an identification and quantitation for each. The HbA2assay separates this minor fraction from all other haemoglobins giving an accurate percentage. Abnormal variants are also separated. To validate the HbA2, assay 252 samples were assayed by the cellulose acetate electrophoresis/elution method and LPLC with a correlation of 0.932. The system provides an accurate and sensitive alternative to traditional manual chromatography and electrophoresis methods. The automated sampler allows batches from 1‐99 samples to be processed with significant savings in operator tim
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00135.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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7. |
Bone marrow fibrosis: histomorphometric analysis and interobserver reproducibility of a simple optical method of assessing its intensity |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 129-135
MIGUEL T. HERNÁNDEZ‐GARCÍA,
LUIS HERNÁNDEZ‐NIETO,
MARÍA L. BRITO‐BARROSO,
GLORIA GONZÁLEZ‐BRITO,
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摘要:
SummaryA histomorphometric analysis of the length of reticulin fibres per area of haemopoietic bone marrow was performed on 59 trephine iliac crest biopsies. The values obtained were found to correlate with the degree of fibrosis as determined by a simple optical method based on the degree of microscopic magnification required for recognition of the presence of reticulin fibres. The mean length of fibre (μm/10000 μm2) for the three degrees of fibrosis defined by the optical method were: 241.8 ± 16.6 for grade I, 713 ± 85.6 for grade II, and 1827.9 ± 230.4 for grade III (P<0.001). In a series of 67 biopsies, the overall interobserver agreement of the optical method was found to be good (Spearman'sr= 0.99;P<0.001) and there was good individual agreement for each of the three degrees of fibrosis (improved kappa test). There was a small amount of overlap between the extreme values of adjacent optical degrees. These results suggest that the optical method described here can be recommended as a practical technique for the routine evaluation of myelofibr
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00136.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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8. |
Infectious mononucleosis complicated by haemolytic anaemia due to the Donath Landsteiner antibody and by severe neutropenia |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 137-140
C. O. UZOKWE,
A. M. GWYNN,
D. W. GORST,
A. R. ADAMSON,
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摘要:
SummaryA young man developed infectious mononucleosis complicated by profound anaemia due to haemolysis. The Donath Landsteiner antibody was found in his serum. He was treated successfully by blood transfusion but subsequently developed severe neutropenia. Both complications have been reported previously but not in the same individcual. No underlying immune deficiency could be identified.
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00137.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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9. |
A case of transfusion‐acquired hepatitis C |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 141-144
T. G. WREGHITT,
M. K. GANDHI,
J. J. GRAY,
J. BLAGDON,
A. RANKIN,
P. B. COATES,
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摘要:
SummaryThe Blood Transfusion Service introduced screening for Hepatitis C antibody (HCV) in September 1991. This is done by second generation enzyme linked immunosorbent assay (ELISA) tests. We present a case of post‐transfusion hepatitis C hepatitis in a patient with myeloma. Infection was acquired before screening was introduced. Both the patient and the infected blood donor were diagnosed using ELISA assays and the polymerase chain reaction (PCR). In this way we prevented the blood donor from spreading the virus via subsequent blood donations. There were some interesting discrepancies in the HCV assays. Blood samples, when tested by different methods, gave both positive and negative results. The results also varied according to when the blood samples to be tested were taken. The case illustrates the importance of confirming positive results and that no single laboratory test is entirely satisfactory in diagnosing HCV infectio
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00138.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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10. |
Factor VIII inhibitor associated with hepatocellular carcinoma |
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Clinical&Laboratory Haematology,
Volume 15,
Issue 2,
1993,
Page 145-148
R. F. NEILSON,
I. D. WALKER,
M. ROBERTSON,
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ISSN:0141-9854
DOI:10.1111/j.1365-2257.1993.tb00139.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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