ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00087.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SummaryOne‐hundred and four patients with a diagnosis of aplastic anaemia (28) or neutropenia (76) referred to our laboratory for assessment of granulopoicsis were studied. Bone marrow myeloid progenitor cell (CFU‐C) frequency was measured and in 85 patients, inhibitor studies with either in‐vitro drug addition or plasma co‐culture were performed. Of 28 patients with aplastic anaemia, 22 (79%) had low numbers of CFU‐C, while four (14%) had numbers within the normal range and two (7%) had elevated progenitor cell frequency. In contrast, of the 76 patients with neutropenia who were studied only 29 (38%) had low CFU‐C numbers, 33 (43%) had a CFU‐C frequency within the normal range and 14 (18%) had elevated CFU‐C numbers. Thirty‐nine patients had ingested potentially myelotoxic drugs and in eight of these it was possible to demonstrate drug associated inhibition of CFU‐C proliferationin vitro. The drugs most commonly associated on a historical basis with myelosuppression were chloramphenicol, antimalarials, sulphonamides, anticonvulsants and nonsteroidal anti

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00088.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SummaryProtein C activity and antigen levels have been related to clotting activities of factors VII and X during the induction and withdrawal periods of oral anticoagulant treatment. Both factor VII and protein C activities fell rapidly during induction but factor VII showed a more rapid and much more marked depression than protein C. In contrast, reductions in factor X were much slower. Protein C antigen, although depressed rapidly at the initiation of treatment, did not subsequently fall to the same degree as protein C activity. The ratio of activity to antigen became progressively smaller. On discontinuation there was a reversal of the pattern but with two important differences. Firstly, there was evidence of an excessive rise (‘rebound’) of factor VII compared with the steady state levels in these patients; and secondly there was a surprisingly slow return of protein C to normal levels after the oral anticoagulant was withdrawn (levels were still below normal on day 4). These observations lend support to gradual withdrawal of oral anticoagulants after a period of long‐term administration. The results suggest that after discontinuation of long‐term anticoagulants patients may have increased coagulability up to fo

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00089.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SummaryHaemoglobin concentration and other blood cell indices were determined in a Coulter counter (Model S‐Sr) in order to establish a reference range for healthy Hong Kong Chinese adults. The mean values for all the blood cell indices were significantly higher (P0.01), PVC (P<0.05) and Hb concentration, the latter not reaching statistical significance. The MCH, however, increased after 70 years of age (P<0.05). The reference ranges derived for healthy Hong Kong Chinese adults are of a similar order of magnitude to those already publis

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00090.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SummaryAfter interaction with human macrophages derived from blood, bone marrow or spleen, solutions of sodium phenobarbitone, phenytoin sodium and chlorpromazine hydrochloride showed reduced cytotoxicity towards K562 cells. The reduction in cytotoxicity was partially suppressed in the presence of telrahydrofurane, an inhibitor of cytochrome P450. These data suggest that macrophages are capable of metabolizing certain drugs, probably via a cytochrome P450‐dependent mechanism. The present findings raise the possibility that some drug‐induced blood dyscrasias are caused by metabolism of the drug by bone marrow macrophages and the consequent release of relatively short‐lived molecules which are toxic to adjacent haemopoietic cells. The generation of cytotoxic molecules during drug metabolism by macrophages may also be responsible for drug‐induced damage to other macrophage‐rich tissues. In addition, since cytochrome P450‐dependent reactions seem to occur within macrophages, these cells may activate environmental procarcinogens and thus plays a role in carcinogenesis and leuk

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00091.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SummaryFifteen patients with chronic benign idiopathic neutropenia (CBIN) with neutrophil countsfour‐fold increase in the remaining nine patients. The findings suggest that the benign course of CBIN is not due to excess neutrophil margination nor to compensatory monocytosis, but that at least one mechanism includes a functionally adequate release of neutrophils to the pcripheral blood under conditions of stress with subnormal delivery of ncutrophils under basal conditions. The variability in responses to endotoxin suggests that CBIN is not entirely homogeneous with respect to mechanism. The findings of relatively normal bone marrow cellularity and differential counts and a normal or substantial neutrophil response to endotoxin appear characteristic. They may help predict a benign clinical course in neutropenic patients and assist diagnosis of the CBIN variant of idiopath

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00092.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SummaryThe Technicon H6000 system simultaneously determines blood cell and leucocyte differential counts automatically using cytochemistry and cell size measurements. Part of the information provided by this instrument is a plot of peroxidase staining intensity against leucocyte cell size called ‘the leucocyte peroxidase distribution display’. Recently, an abnormal leucocyte peroxidase distribution display was observed, on a sample from a post‐operative lung cancer patient, in which eosinophilic leucocytes did not react with peroxidase. Neutrophils reacted in a normal way with this sample. The eosinophils were further examined by electron microscopy and manual peroxidase staining. Some peroxidase positive granules were observed in the eosinophilc leucocytes. The abnormal leucocyte peroxidase distribution display was found to be due to a separate group of eosinophilic leucocytes with lowered peroxidase act

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00093.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SummaryTo determine whether a simple immunoperoxidase (IP) technique could replace the traditional immunofluorescence (IF) technique (by fluorescence microscopy) for determination of phenotype and monoclonality in cell suspensions from patients with lymphoprohferdtive disorders, we tested samples from 48 cases by both methods, using small panels of antibodies designed to determine proportions of B‐cells, T‐cells and cells bearing immunoglobulin light chains. There were 27 cases of chronic lymphocytic leukaemia (CLL), 6 of prolymphocytic leukaemia (ProLL), and 15 of non‐Hodgkin's lymphoma (NHL). We found that IP was at least as reliable as IF in all cases. In the 47 B‐ cell cases, the mean proportion of cells apparently belonging to the malignant clone was higher by IP than by IF; conversely, the proportion of B‐cclls displaying the non‐malignant light chain marker and the proportion of residual T‐cells was apparently lower by IP. We conclude that IP is a useful and reliablc tcchnique for determining phenotype and monoclonality in lymphoproliferative disorders; because it is tolerant of delays to samples in transit and requires only simple facilities, it may be particularly suitable for small

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00094.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SummaryA blind trial conducted amongst 29 laboratories revealed that the overall incidence of ‘false‐positive’ results in tests for anti‐platelet antibodies was greater for heat‐treated serum and plasma samples than for untreate

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00095.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SummaryThe Cell‐Dyn 1000 is a new eight parameter fully automatic cell counter. This was evaluated following the ICSH guidelines. Scientific evaluation was generally satisfactory, largely conforming to the manufacturer's specifications. A number of potential microbiological safety hazards were noted; many could be overcome by minor modifications. Throughput and start‐up/close‐down times were acceptable. However, the reliability of the tested machine was felt to be less than satisfactory although this may be corrected by further minor machine modifica

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1987.tb00096.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY