ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00559.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Summary.The design of exercises of compatibility testing was modified in 1981 in order better to accommodate participants' serological practices. Ten reference laboratories were also enrolled in order to determine the ‘correct’ results for each exercise. In 1981–1982 3.5 to 36% of participants missed incompatibilities in exercises in which undiluted antibodies were issued and this did not represent an improvement over performance obtained in 1979–1980. Surveys were undertaken of antiglobulin test procedures and revealed that serological practices continue to change, old techniques are being modified, new techniques are being employed but standardization shows little overall improvement. Surveys of quality control procedures and of cross‐match procedures for agglutination in albumin and for agglutination of enzyme treated cells show equal lack of standa

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00560.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Summary.Immunoglobulin G (IgG) boundin vivoto the surfaces of red blood cells (RBC‐IgG) was quantificated by an enzyme‐linked immunosorbent assay (ELISA) using the cells themselves as solid phase. The method was applied on RBC from normal subjects, patients with autoimmune haemolytic anaemia (AIHA) and rheumatoid patients with and without circulating immune complexes (CIC). Small amounts of RBC‐IgG were detected in normal subjects and rheumatoid patients without CIC. Fifteen out of 16 patients with AIHA had increased RBC‐IgG indicating RBC sensitization with IgG antibodies, although only eight patients had a positive direct antiglobulin test (DAT) with anti‐IgG. Ten out of 13 rheumatoid patients with a negative DAT and with CIC had increased RBC‐IgG suggesting RBC C3 receptor‐bound IC. The results provide background for further studies of the significance of RBC‐IgG in hea

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00561.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Summary.Ten sera containing polyspecific anti‐HLA antibodies, a rabbit anti‐human leucocyte antiserum and a monoclonal anti‐granulocyte antibody were used in the development of a semi‐quantitative technique for the detection of anti‐granulocyte antibodies. The assay measures the metabolic response of human monocytes to antibody coated (opsonized) granulocytes. Immune complexes were opsonic only in the presence of fresh human serum. Monocyte response was strongly inhibited by normal human serum and purified free immunoglobulin. The assay is simple, rapid and sensitive, and all antibodies investigated were readily

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00562.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Summary.Seven children, referred because of bleeding symptoms, proved to be heterozygotes for factor VII deficiency. Abnormal bruising was the commonest symptom (6/7), followed by postoperative bleeding (4/7). One case had recurrent epistaxis. Results on the patients and their families are presented. Prothrombin times were prolonged by 2–3 s and factor VII assays ranged from 25 to 55% (mean 38%). The cases are reported because they bled excessively: yet it is usually stated that carriers for factor VII deficiency are symptom‐free. One case of homozygous factor VII deficiency is also described: the parents (who are heterozygotes) were symptom

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00563.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Summary.A questionnaire survey of current practice in the bleeding time test has been undertaken by the UK External Quality Assessment Scheme in blood coagulation. Completed returns have been received from 358 centres. Most centres (88.5%) perform bleeding times and of these the Ivy test is the most commonly performed. Only 13.6% perform the Duke method. Templates are used to control the procedure by approximately half of the hospitals. There is considerable variability in the type and depth of incision and interpretation of the endpoint. The upper limit of normality not unexpectedly differs considerably between the centres with both Ivy and Duke methods. The use of a commercial template method, ‘Simplate’, provides a measure of agreement amongst the group of hospitals using this instrument but it remains to be established whether this is the most reliable procedure. In the interim, gross discrepancies in technique or interpretation should be corrected in the light of the findings of the sur

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00564.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Summary.Because of poor venous access and failure of previous arteriovenous shunts due to thrombosis, two patients with severe coagulapathies had an arteriovenous fistula performed using a new chemically treated bovine carotid artery segment (Solcograft‐P). To date, no complications have been noted after 16 and 17 months respectively. This treated heterograft maintains many of the physical characteristics observed in a normal bovine carotid artery. The theoretical advantages for using this heterograft in such patients are discusse

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00565.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00566.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Summary.By means of immunoperoxidase histochemistry through monoclonal antibodies, OKT3+T cells were detected in the cutaneous lesion of a patient with Sezary syndrome. The majority of the infiltrating cells showed positive staining with OKT4 helper cell marker whereas a few OKT8+cells were also seen. Many OKT6+Langerhans' cells accumulated in the epidermis overlaying the dermal infiltrate.

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00567.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1984.tb00568.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY