摘要:

Summary.A family is described in which the father and son had erythrocytosis associated with a normal Hb oxygen affinity. Growth of erythroid coloniesin vitro(BFU‐E) exhibited normal erythropoietin dependence. In the son there was an enlarged erythroid precursor compartment, while the father (who had been treated by busulphan) showed marked reduction of circulating BFU‐Es. Serum erythropoietin (Epo), estimated by radio‐immunoassay, was 96 miu/ml in the son and 360 miu/ml in the father (normal 25, SD 6,n= 46). We conclude that erythrocytosis in this family is due to a genetically determined hyper‐production of Epo. The finding in the father of a high Hb level associated with increased Epo and decreased BFU‐Es might support the hypothesis that red cell mass is regulated by Epo at the level of bone marrow CFU‐Es rather

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00506.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Summary.The French‐American‐British (FAB) classification of acute lymphocytic leukaemia (ALL) has recently been modified to improve its reproducibility. We therefore thought it beneficial to test the utility of this modified classification in the prognosis of children with ALL. Eighty‐eight patients of less than 18 years of age with ALL were subtyped according to the modified FAB classification by two independent observers. The initial concordance was 86%. Seventy per cent of the patients were subtyped as LI, 27% as L2 and 3% as L3. Patients with L3 subtype were not analysed further. No significant differences were observed between Ll and L2 subtypes when the distribution of other prognostic factors were examined. No differences were observed between L1 and L2 subtypes in the achievement of complete remission. Patients with L1 morphology demonstrated no significant increased duration of first remission or survival when compared to patients with L2 morphology. We conclude that the morphology oflymphoblasts may not be an independent prognostic variable in patients wit

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00507.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Summary.Ten individuals with idiopathic neutropenia and similar numbers of normal and abnormal controls were tested for mobilization of their marginal granulocyte pools and bone marrow reserve by using epinephrine and hydrocortisone intravenously. Individuals with ‘benign’ idiopathic neutropenia appeared to have a normal response while half the abnormal controls responded poorly. It is suggested that granulocyte mobilization tests are valuable in the assessment of individuals with neutrope

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00508.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Summary.A simple, low pressure, blood scavenging system has been assessed in major abdominal vascular operations. The aim of this study was to assess the quality of blood obtained with this device and its suitability for reinfusion. Three areas of special interest, which have not been reported so far, are the fate of plasma complement, the load of fat aspirated after tissue dissection and the degree of bacterial contamination in the scavenged blood. The development of autologous blood scavenging systems is reviewed. The features which most affect the quality of scavenged blood are identified and their importance discussed by comparison with our experience. Key features of our system were: simplicity of the apparatus, controlled low‐pressure aspiration, the use of systemic heparin and the avoidance of mechanical pumps. The blood obtained was of excellent quality with good preservation of cellular elements, platelets and fibrinogen. Plasma total haemolytic complement, C3, and C4, fractions were preserved in normal, though slightly reduced, quantities. Lipid (triglyceride) content was minimally increased after filtration. Bacterial contamination was present in all cases, but at a very low level provided that aspiration was limited to the peritoneal cavity. This low‐level of contamination is not thought to be of great significance; its origin and importance are discu

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00509.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Summary.A 42‐year‐old leukaemic black male developed shortness of breath, itching, hot flushes, substernal pain, marked hypotension, and cardiorespiratory arrest after the infusion of 50 ml of pooled platelet concentrates. The patient had had one previous and uneventful red blood cell transfusion. Serologic studies of this patient's post‐transfusion serum showed the presence of an antibody against one denatured IgA2 protein with the phenotype A2m(l+2‐). All of the platelet donors were A2m(1) positive. The patient was successfully transfused with platelets from donors with the phenotype A2m(l‐2+). Although 35.8% of Blacks and 30.0% of Chinese are A2m(l) negative, this is the first reported transfusion reaction associated solely with the occurrence of a possible anti‐A2m(1) in an individual of the type

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00510.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Summary.Measurements have been made of cross‐match/transfusion ratios (C/T ratio) and intervals between successive cross‐matches on the same units of blood performed in three different major hospitals each providing a wide range of clinical services. Outdate rates were 4%, 15% and 25% respectively and were associated with C/T ratios of 1.3, 2.0 and 2.15. There were corresponding increases (means of 3.6, 4.5 and 5.0 days) in the length of time unused units of blood were left between successive cross‐matches. Calculations of outdate rates from the above figures closely predicted the trend shown by observed non‐use rates. It has thus been demonstrated that apparently small differences in blood bank practice can produce important variations in the efficiency of use of red cel

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00511.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Summary.Summary A sensitive method is described for the quantification of C3 fragments on erythrocytes. A radiolabelled monoclonal antibody, was used which was directed against a C3d determinant on all forms of cell bound C3. The number of C3 molecules on normal erythrocytes was estimated to be 420±140. The strength of the antiglobulin test increased from negative to 5 + over a range of only 850 C3 molecules (400‐1250). A blood donor with a positive direct antiglobulin test was found to have 4800 molecules per cell whereas three cases of cold haemagglutinin disease with active haemolysis had from 16000 to 52020 C3 molecules per cell. This test has an application in the testing of acquired haemolytic anaemia cases with a positive direct antiglobulin test with C3 bound to the cells and in the standardization of sensitized cells used for testing antiglobulin reagents by various serological techniqu

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00512.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Summary.The Coulter Counter Models S Plus II and III have been evaluated. No serious safety hazards were identified. Scientific assessment showed some non‐linearity in the Hb which caused the MCH and MCHC to vary as samples were diluted. Precision and carry‐over were satisfactory. The results obtained compared well with those of the Coulter Counter Model S, except for WBC; reference methods showed better accuracy on the Models S Plus I1 and III. Platelet counts agreed with those by phase‐contrast microscopy and the lymphocyte percentage was similar to that from the blood film except in the lymphoproliferative disorders. The whole blood and pre‐dilute modes gave similar results though the platelet count was slightly higher in the whole blood mode. In the National External Quality Assessment Scheme results were in accordance with those from other Model S Plus Users. Time did not allow a detailed evaluation of the cell volume distribution curves but it was noted that the white cell profile was useful for detecting platelet aggregation. Efficiency assessment showed throughputs of 66 and 93 samples per hour on the Models S Plus II and III respectively. The platelet count was clinically useful as was the lymphocyte percentage measurement which rendered some differentials unnecessary. Rejection of the white cell profile was a helpful index of abnormality on the Model S Plus II but occurred non‐specifically on the Model I

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00513.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Summary.A study has been performed to evaluate the suitability of Vacutainer tubes in blood specimen collection for coagulation tests and to compare them with the conventional syringe technique employed in UK hospitals. Blood was collected from healthy volunteers, an ante‐natal group and patients on long‐term oral anticoagulants. Samples were stored at two different temperatures; 4|MoC and ambient room temperature (RT). Prothrombin times, factor VII assays and APTT were performed at baseline and after 2 h and 4 h storage. There was significant activation of the extrinsic system in the blood samples collected by Vacutainer when stored at 4|MoC which became more significant on prolonged storage. The effect was less pronounced when the Vacutainer tubes were stored at RT. In contrast, the blood collected by the syringe method did not show these changes with the exception of the ante‐natal specimens where a lesser degree of activation than in the Vacutainer tubes was observed after 4 h at 4|MoC. The activation of the Vacutainer samples at 4|MoC is considered undesirable and could be of clinical significance in oral anticoagulant d

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00514.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1983.tb00515.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY