摘要:

SummaryThe pattern of changes in neutrophil myeloperoxidase (MPO) before, during and after bacteraemia was studied in 34 patients recovering from autologous bone marrow transplantation for relapsed Hodgkin's disease and non Hodgkin's lymphomas. Thirteen patients received haemopoietic growth factors (7 received M‐CSF, 3 received G‐CSF and 3 GM‐CSF). The mean peroxidase index (MPXI) produced as part of a routine FBC performed by a flow cytochemistry blood autoanalyser (Technicon H*1) was used as a parameter to assess the MPO and subsequently the azurophil degranulation. The manufacturer's normal values for MPXI range from −10 to +10. Median MPXI on the day of documented bacteraemia was just below normal in the control and M‐CSF groups (−10.8 and −8.9 respectively), but it was much below normal in the G‐CSF (−16.5,P<0.05) and even lower in the GM‐CSF group (−39.6,P<0.02); this correlated well with the decreased bacteraemia incidence in the last two groups. Although contact of neutrophils with bacterial chemoattractants resulted in primary degranulation in all groups, the pattern of changes in MPO content was different, suggesting that neutrophils primedin vivowith various haemopoietins respond to the challenge of microbial agents v

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00102.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryTo study the effects of cigarette smoking and atherosclerosis on platelet size, we measured the mean platelet volume (MPV) and other platelet parameters in 142 elderly smokers and nonsmokers with or without atherosclerotic risk factors. The MPV and the platelet count were highest and their inverse correlation was strongest in the atherosclerotic smokers (r= 0.54,P<0.05) when compared with the nonsmoking and non‐atherosclerotic groups. A 10% decrease of MPV was found in 8 smoking subjects in the atherosclerotic group, who successfully discontinued smoking (P<0.05). These results suggest that smoking may increase platelet consumption in atherosclerotic vessels and that subsequently megakaryocytes are activated to produce larger platelets, which are more active. Thus, an increase in MPV due to smoking may also contribute to the acceleration of atherosclerosis and should be considered as a risk factor for atherosclerotic diseas

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00103.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryIn this paper we report an unusual Sardinian family, in which the heterozygosity for β∘39‐thalassaemia and for triple α‐globin gene complex have been found in two members: the former showing a high HbA2mild thalassaemia intermedia syndrome, the latter, her daughter, showing a normal HbA2thalassaemia trait. Molecular analysis revealed the daughter to also be a carrier of a δ+27‐thalassaemia point mutation, which intransto the β∘39defect invariably normalizes t

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00104.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThis study uses the statistical methods of simple and multiple regression to compare the differential white blood count on the Coulter VCS and Technicon HI analysers. The results demonstrate that both are good at distinguishing lymphocytes, neutrophils and eosinophils. Monocyte differential counts show disappointing correlation, both by simple and multiple regression techniques. Basophils, though less frequently a clinical problem, also correlated poorly in this study.

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00105.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryWhen dipotassium EDTA anticoagulated platelet rich plasma was incubated against a range of hypotonic saline concentrations, platelet volume was seen to change in a particular and rcproducible fashion. When platelets taken from patients suffering a thromboembolic episode were tested in the same manner, a different pattern of response was observed the platelets being far less tolerant to osmotic stress. Similar changes occurred in platelets from ante‐natal subjects. Platelets from both groups regained their tolerance to hypotonicity following either the thromboembolic incident or parturition respectivel

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00106.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe chemiluminescent (CL) response of interferon‐γ‐treated U937 (IFN‐U937) cells to sensitized target cells has been used to detect red cell, platelet and granulocyte antibodies. A clone of U937 cells was selected which expressed Fc receptor I (FcγRI) and which, after incubation with IFN‐γ for 72 h, was capable of generating high levels of lucigenin‐enhanced CL. The CL responses of IFN‐U937 cells and peripheral blood human monocytes to sensitized red cells, platelets or granulocytes were then compared. Assays using monocytes or IFN‐U937 cells were of comparable sensitivity for detection of antibodies against all three types of target cell. In addition, the use of IFN‐U937 cells reduced interassay variation and simplified assay performance. The potential clinical usefulness of these CL assays was suggested by the ability of both monocytes and IFN‐U937 cells to respond to red cells, platelets or granulocytes sensitized with sera from pregnant women whose babies had either haemolytic disease of the newborn (HDN), alloimmune thrombocytopenia or alloimmune neutropenia respectively. In addition, monocytes and IFN‐U937 cells both responded to red cells sensitized with antibodies against a variety of specificities of assumed (although not documented) clinical significance for blood transfusion recipicnts. In contrast, monocytes and IFN‐U937 cells responded only weakly to red cells sensitized with either anti‐D in sera from mothers of babies unaffected by HDN, or with antisera containing high titre antibodies with specificities not normally associated with significantly

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00107.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00108.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00109.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00110.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1992.tb00111.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY