1. |
A pyruvate kinase variant with an increased affinity for phosphoenolpyruvate in an individual with congenital non‐spherocytic haemolytic anaemia |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 237-241
O. SMITH,
I.J. TEMPERLEY,
K. TIPTON,
S. R. McCANN,
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摘要:
SummaryA red‐cell pyruvate kinase (PK) variant from an individual with congenital non‐spherocytic haemolytic anaemia was characterized according to the procedure recommended by the International Committee for Standardization in Haematology (ICSH 1979). Activity of the mutant enzyme in haemolysates was one fifth of that found in normal control subjects. The electrophoretic mobility and thermostability of the mutant enzyme were lower than those of the normal enzyme and its apparent affinity for phosphoenolpyruvate was hig
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01359.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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2. |
Circulating plasmin‐antiplasmin complexes in acute leukaemia |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 243-251
M.J. GALLOWAY,
M.J. MACKIE,
B.A. McVERRY,
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摘要:
SummaryThirty consecutive patients presenting with acute leukaemia were studied throughout their hospital course to determine if plasmin‐α2antiplasmin (P‐AP) complexes could be detected during episodes of increased fibrinolytic activity and to correlate this finding with other more conventional laboratory parameters. Increased fibrinolytic activity was a common finding, it was detected in 19 (63%) patients. This was usually present at diagnosis, but occasionally occurred later as a transient phenomenon. Increased fibrinolytic activity could not clearly be associated with either infection or chemotherapy. P‐AP complexes were found in 11 (37%) patients and were almost always accompanied by additional laboratory evidence of increased fibrinolysis. These complexes were present in most instances at diagnosis and disappeared following successful chemotherapy. Forty‐five per cent of patients with P‐AP complexes had low α2antiplasmin levels and 36% had low plasminogen levels which returned to normal following successful chemotherapy. At diagnosis six of eight patients with P‐AP complexes had major haemorrhagic m
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01360.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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3. |
Acquired basophil and eosinophil deficiency in a patient with hypogammaglobulinaemia associated with thymoma |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 253-257
E.B. MITCHELL,
T.A.E. PLATTS‐MILLS,
R.S. PEREIRA,
VERA MALKOVSKA,
A.D. WEBSTER,
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摘要:
SummaryA 55‐year‐old male with a history of recurrent bacterial and viral infection was found to have hypogammaglobulinaemia in association with a benign thymoma. The patient lacked eosinophils and was deficient in basophils in both the peripheral blood and bone marrow. The absence of eosinophils in a skin challenge known to recruit these cells, and below normal total peripheral blood leucocyte histamine content further suggests a deficiency of eosinophils and basophils respectively. Abnormal suppressor T cell function was documented both phenotypically, using OKT monoclonal antisera, and functionally in pokeweed mitogen stimulated cultures. This patient is the first reported with a deficiency of both eosinophils and basophils occurring in the presence of hypogammaglobulinaemia and thymoma; these abnormalities may be related to the abnormal suppressor T cell function that is pres
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01361.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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4. |
Massive retroperitoneal lymphadenopathy as a terminal event in hairy cell leukaemia |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 259-263
A.B. MEHTA,
D. CATOVSKY,
C.J. O'BRIEN,
M. LOTT,
N. BOWLEY,
A. HEMMINGWAY,
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摘要:
SummaryA case of hairy cell leukaemia complicated as a terminal event by massive retroperitoneal lymphadenopathy is described. The patient had recently been treated with lithium carbonate and had previously been demonstrated to suffer from a systemic vasculitis, either or both of which may have contributed to the development of this rare complication.
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01362.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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5. |
5′‐nucleotidase activity in myeloma cells |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 265-269
E.A‐M. EL‐MOHANDES,
F.G.J. HAYHOE,
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摘要:
SummaryPlasma cells were studied cytochemically for 5′‐nucleotidase (5′‐N) activity in bone marrow smears from 11 patients with multiple myeloma and 12 cases with no haematological disorders but with adequate numbers of plasma cells. The activity of 5′‐N could be demonstrated in about 90% of myelomatous plasma cells. The non‐myelomatous plasma cells of control smears were negative for 5′‐N activity. The activity of 5′‐N did not seem to be affected by storage of unfixed smears for up to 5 month
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01363.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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6. |
Comparison of methods used in commercial kits for the assay of serum vitamin B12 |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 271-277
D.I. FISH,
D.W. DAWSON,
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摘要:
SummaryFourteen commercial kits for serum vitamin B12assay have been assessed. The analytical performance and clinical correlation were used to rank the methods employed for the extraction of the vitamin from its binders and for the separation of ‘bound’ from ‘unbound’ counts. The introduction of extraction without boiling and the separation by attachment of the vitamin B12binder to a solid matrix have not been shown to be generally better or worse than conventional
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01364.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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7. |
A study of the routine use of the Imugard IG500 Leukocyte Removal Filter illustrating a possible cause of transfusion reactions and a method of prevention |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 279-285
M. McGregor,
J.F. Harrison,
R. KIRKHAM,
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摘要:
SummaryThe results of the use of the Imugard IG500 Leukocyte Removal Filter in the transfusion of 100 patients requiring leucocyte poor blood are presented. The patients were suffering from a variety of disorders. Many of them had previously received leucocyte poor blood prepared by other methods. Five patients developed non‐haemolytic transfusion reactions. Subsequent investigations suggested that these were reactions to the plasma proteins present in the leucocyte poor blood prepared by filtration. A simple method of removal of these plasma proteins combined with filtration is described. The resultant product has been transfused, without incident, into the patients who previously reacte
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01365.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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8. |
Five‐day storage of platelet concentrates. II.In‐vivostudies |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 287-294
H.H. GUNSON,
A.H. MERRY,
Y. MAKAR,
E.E. THOMSON,
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摘要:
SummaryFollowingin‐vitrotests it was concluded that platelet concentrates stored for 5 days at 22°C in polyolefin containers, coded PL732, should be as effective in clinical practice as similar concentrates stored in the standard PVC containers, coded PL 146. These predictions have been confirmed by the followingin‐vivotests; autologous survival studies in volunteers, determination of recovery, platelet increment calculations 1 and 24 h after transfusion and clinical appraisal after transfusion of haemorrhagic thrombocytopenic patients. Bacteriological cultures of the platelet concentrates were sterile after storage for more than 5 days. It can be concluded that the 5‐day storage of platelet concentrates in these containers is a practical propo
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01366.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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9. |
A comparison of PVP and methyl cellulose as rouleaux inducing reagents in the automated anti‐D quantification method |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 295-301
P.J. BOWELL FIMLS,
MARTHA K. SALATA MT (ASCP),
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摘要:
SummaryThe anti‐D potency of 102 samples was determined using the automated anti‐D quantification method. On the whole the values obtained when using PVP as the rouleaux inducer were no different to those obtained using methyl cellul
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01367.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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10. |
The Rh antigen Tar (Rh40) causing haemolytic disease of the newborn |
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Clinical&Laboratory Haematology,
Volume 5,
Issue 3,
1983,
Page 303-305
C. LEVENE,
R. SELA,
L. GRUNBERG,
R. GALE,
C. LOMAS,
P. TIPPETT,
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摘要:
SummaryHaemolytic disease of the newborn (HDN), in the fifth child of an Israeli of Moroccan origin, disclosed anti‐Tar in the serum of the mother. This anti‐Tar, the second reported example, is the first to cause HDN and was stimulated by previous pregnancies. Tests on blood from the family show that the Tar antigen in this family is associated with aCDecomplex which gives rise to a weak expression of the D antigen. A study of 1327 donors in Jerusalem revealed one Tar + proposi
ISSN:0141-9854
DOI:10.1111/j.1365-2257.1983.tb01368.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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