摘要:

In cultured human skin fibroblasts the glucose flux through the hexose monophosphate shunt (HMS) amounts to 4% of the glucose flux through the glycolytic pathway. Upon in vitro ageing the rate of glucose utilization through the HMS is decreased more than 50%. This decrease in HMS was not caused by a limiting enzymatic capacity since glucose utilization through the HMS could be raised at least 30-fold in both ‘young’ and ‘aged’ fibroblasts upon stimulation with phenazine methosulphate. This effect of in vitro ageing upon glucose metabolism was also not due to differences in proliferation rate between ‘young’ and ‘aged’ human fibroblasts, since there was no difference in glucose utilization between proliferating and growth-inhibited (confluently cultured) fibroblasts. The NADPH/NADP ratio was found to be decreased by 12%

ISSN:0304-324X    

DOI:10.1159/000212891

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

We describe the investigation of the content of chromosomal high-mobility group 1 (HMG1) protein in three cellular fractions (cytosol, microsomal, and nuclear) of the magnum and shell gland of young and aged hen oviducts. In the magnum of the aged hen oviduct, the amount of the HMG1 protein (μg protein/mg DNA) in the three fractions was 9.3 ± 1.1,0.18 ± 0.03, and 2.4 ± 0.4, respectively. Whereas the values were 11 ± 1.2,0.27 ± 0.08, and 4.2 ± 0.4, respectively, for the young hen. 12, 50, and 43% less were found in the aged hen magnum. In the shell gland of the functionally active hen oviduct, the protein in the three cellular fractions was 8.6 ± 1.05, 0.42 ± 0.06, and 3.9 ± 0.55, respectively. In the shell gland of the functionally inactive aged hen, the values were 8 ± 1.05, 0.28 ± 0.09, and 0.85 ± 0.12, and in the inactive shell gland of the young hen, they were 7.6 ± 1.0, 0.27 ± 0.09, and 1.75 ± 0.22, respectively. The most significant difference was in the nuclear fraction, where the HMGi protein in the functional hen shell gland was 123–359% higher than that of

ISSN:0304-324X    

DOI:10.1159/000212892

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Plasma somatostatin levels were determined during a 24-hour period and after a meal test in 7 and 5 elderly subjects (76–90 years), respectively. The data obtained were compared with those recorded in young adult subjects (22–30 years). Increased basal somatostatin values were found in elderly subjects (20.0 ± 1.5 pg/ml) when compared to young adults (14.1 ± 0.6 pg/ml; p < 0.01). Also, the mean 24-hour somatostatin levels were higher in the elderly (21.3 ± 0.8 pg/ml) than in young adults (16.7 ± 0.5 pg/ml; p < 0.01), and minor diurnal variations were found in the former group. The response to the meal test was less evident in the elderly than in the control group. The data obtained indicate an increased basal somatostatin production associated with a diminished variability throughout the 24-hour period and in relation t

ISSN:0304-324X    

DOI:10.1159/000212893

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

A woman treated with fluoride for corticosteroid-induced osteoporosis presented 1 year later with an unusual localized supraacetabular followed by a same-sided femoral head fracture. Fluoride was increased in serum and urine. Transiliac bone biopsy revealed typical bone fluorosis with elevated trabecular bone fluoride.

ISSN:0304-324X    

DOI:10.1159/000212894

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

A previous study found hyposomatomedinemia to be common in the men of this VA Nursing Home. To gain information on the prevalence and correlates of this endocrine characteristic, we have measured plasma somatomedin C (SmC) in 69 Nursing Home men 55–95 years old (group I), and in 37 independent men 56–87 years old attending our geriatric medicine outpatient clinic (group II). In groups I and II, a clinical data base was compiled comprising: blood chemistries, measures of body composition – nutritional state and functional level, diagnoses, medications, and morbidity and mortality during the year after the SmC analysis. The mean ± SD for SmC was significantly (p < 0.05) lower in group I (0.35 ± 0.21 units/ml) than in group II (0.45 ± 0.13 units/ml). SmC less than 0.25 units/ml, a range consistent with severe growth hormone deficiency, was found almost exclusively in group I (31.8% of men in group I, 3.7% of men in group II). In either group I, or in groups I and II combined, SmC was significantly (p < 0.05) correlated with body weight as percentage of ideal, midarm muscle circumference (MAMC) as percent of standard, diagnosis of cerebrovascular disease, and plasma testosterone level. Men with SmC values below 0.25 units/ml had significantly (p 0.05) with diagnosis, drugs, morbidity or m

ISSN:0304-324X    

DOI:10.1159/000212895

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Asymptomatic autoimmune thyroiditis (AAT), especially prevalent in elderly women and associated with the presence of thyroid antibodies (HT), is recognized as a precursor of hypothyroidism and is an intermediate between euthyroidism and hypothyroidism. This paper is concerned with the possible modifications of biochemical variables – some of which are not directly related to the thyroid – in patients with AAT. Thirty-seven serum factors were investigated in euthyroid, AAT, and HT subjects over 69 years of age. They were thyroglobulin; microsomal, gastric and adrenal antibodies; LE cells, and thyroid, lipid and immunological variables linked to the inflammatory process. In women the only variable, other than those related to the thyroid, which showed any modification in the AAT state was the cholesterol level. Wholly different observations were made in men, stressing the necessity of separate analysis of male and female data: the thyroid-related variables which were modified in the AAT state are not the same as in women, the cholesterol is not increased but triglycerides, HDL, albumin and inflammatory variables are modified. Furthermore, the lipid variables showed an unexpected age dependency in AAT, but not in euthyroid women (between 69–90 years of

ISSN:0304-324X    

DOI:10.1159/000212896

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Injection of dilute phenol to peripheral nerves or motor block areas when there is spasticity, can, by damage to the nerves or motor areas, relieve the spastic condition, allow better nursing care, free the patient from the embarrassment of a contorted limb and may allow voluntary movement to take place.

ISSN:0304-324X    

DOI:10.1159/000212897

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Fifty-seven elderly patients (35 males, 22 females; age range 65–80 years, mean 70 years) with acute myocardial infarction received thrombolytic therapy within 8 h using either streptokinase or anisoylated plasminogen streptokinase activator complex. Coronary artery reperfusion was confirmed by early coronary arteriography. The overall reperfusion rate was 77%. Accurate non-invasive markers of reperfusion included: a rapid fall in the S–T segment elevation, an early peak of the creatinine phosphokinase enzyme curve and reperfusion arrhythmias. Clinical events suggesting early reocclusion occurred in 30%. Coronary arteriography was accompanied by local bleeding associated with the arterial puncture site in 2 patients and a cerebrovascular accident in 1 patient. Minor bleeding occurred in 6 other patients. In those where reperfusion occurred, the mortality at 1 month was 6.6% and in those who did not reperfuse or had early reocclusion 38%. At 1 year the figures were 10 and 46%, respectiv

ISSN:0304-324X    

DOI:10.1159/000212898

出版商:S. Karger AG    

年代:1987

数据来源: Karger

ISSN:0304-324X    

DOI:10.1159/000212899

出版商:S. Karger AG    

年代:1987

数据来源: Karger