摘要:

Fibroblast cultures were established from skin biopsies from 4 male Werner’s syndrome patients, aged 45–50. Several subcultures were obtained from each primary outgrowth and these were passaged to the end of their in vitro lifespan. Their average longevity was significantly less than control skin fibroblasts, but there was extreme variability amongst parallel cultures. In the most thoroughly studied case, the longevity of 19 subcultures derived from a single biopsy varied from 5 to 26 passages, showing that there was considerable heterogeneity in the growth potential of the cells in the primary culture. Moreover, the growth rate from any one culture was not uniform, since long periods of slow growth were sometimes succeeded by much more rapid proliferation. These features of Werner’s syndrome fibroblast populations are not seen in cultures from normal individuals. The longevity of fibroblasts from one progeria patient was also shown to be much shorter than controls. Metaphases from 3 Werner’s patients demonstrated a much higher frequency of chromosome abnormalities than in normal fibroblasts and also provided evidence of subclones containing a characteristic ‘marker’

ISSN:0304-324X    

DOI:10.1159/000213096

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

The lifespan of fibroblasts from genetic syndromes with reduced DNA repair or chromosome stability has been measured. Cells from Bloom’s syndrome, Cockayne’s syndrome, Fanconi’s anaemia and 2 out of 3 cases of ataxia telangiectasia had a significantly reduced growth potential in comparison to controls. In each case the longevity of several parallel populations was measured and the greatest variability in lifespan was observed with Cockayne’s syndrome cells. The fibroblasts from 1 ataxia telangiectasia patient and a Friedreich’s ataxia patient grew to the passage levels seen in control cultures. The results suggest that repair processes are necessary for cells to achieve their maximum in vitro lifespan, and support the error theory rather than the programme theory

ISSN:0304-324X    

DOI:10.1159/000213097

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

The level of heat-labile glucose-6-phosphate dehydrogenase (G6PD) has been measured in skin fibroblast cultures from premature ageing or DNA repair deficient genetic syndromes. The short in vitro longevity of Werner’s syndrome, progeria, Cockayne’s syndrome, ataxia telangiectasia, Fanconi’s anaemia, and Bloom’s syndrome cultures was correlated with the appearance of a significant fraction of heat-labile enzyme. Long-lived control cultures contain a low level of altered enzyme until they become senescent. The evidence that heat-labile G6PD molecules are derived from errors in synthesis, or from other causes, is critically assessed. It is shown that normal cells grown in medium containing the antibiotic, paromomycin, which is known to reduce the fidelity of ribosomal translation, produce a significant fraction of alter

ISSN:0304-324X    

DOI:10.1159/000213098

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

The in vitro longevity of skin fibroblasts in several parallel cultures from each of 4 cystic fibrosis (CF) patients and 3 control patients was measured by serial passages. The control cultures reached 51–56 passages before growth ceased. 1 CF strain achieved an average of 69 passages and another 64 passages. Both of these longevities are significantly greater than the controls. 2 CF strains had significantly shorter longevities than the controls. The results indicate that the CF gene is expressed in cultured fibroblasts and provide further evidence that there may be more than one form of the diseas

ISSN:0304-324X    

DOI:10.1159/000213099

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Mitochondrial respiration with succinate as substrate increased and the mitochondrial protein content declined in the brain of the ageing male garden lizard. The lactate/pyruvate ratio of both blood and brain did not show appreciable changes with advancing age. Cold-shock treatment induced a significant rise in the respiratory rate of brain mitochondria of young but not appreciably in that of middle-aged and old lizards. On the other hand both mitochondrial protein content and lactate/pyruvate ratio increased only in the brain of cold-shocked lizards of the old age-group. The lactate/pyruvate ratio in blood remained unaltered following cold-shock treatment of lizards of all three age-groups.

ISSN:0304-324X    

DOI:10.1159/000213100

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Phytohemagglutinin-induced T cell colony ( > 50 cells/aggregate) and cluster (10–50 cells/aggregate) formation was studied by one-stage microagar culture in glass capillaries by peripheral blood mononuclear cells from 26 young healthy individuals (20–32 years) and 26 aging subjects ( > 65 years). The number of colonies developed after phytohemagglutinin (PHA) stimulation of mononuclear cells from aging subjects was significantly increased (p < 0.05) when compared to those from simultaneously studied young individuals. Increase in the number of PHA-induced colonies by lymphocytes from aging subjects over that of young subjects was observed at different concentrations of PHA. The highest level of colony formation in mononuclear cells from both aging and young subjects was obtained with 10 μg/ml of PHA. Possible mechanisms for increased T cell colony formation in aging humans are discu

ISSN:0304-324X    

DOI:10.1159/000213101

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Sullivan [1982] has stated without reservation that our paper [Tolmasoff et al., 1980] did not present data supporting the possibility that superoxide dismutase has a role in determining longevity in mammalian species. He states that the data instead support the hypothesis that there is no association between superoxide dismutase and lifespan. On reading his arguments, I find no errors in our data analysis or in the conclusion made. Instead, I find he has failed to recognize the critical components of our data and has consequently misinterpreted the results. Because similar oversight might be made by other readers of this paper, I have included a brief review of the rationale underlying our investigation of longevity determinants in mammalian species along with the reply to Sullivan’s criticism

ISSN:0304-324X    

DOI:10.1159/000213102

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

In a representative sample of 624 people, 65–84 years of age, from six regions of Croatia, Yugoslavia, proteinuria was investigated on the basis of certain medical characteristics. Established proteinuria was seen in 30.8% of females and 27.4% of males. Proteinuria was found to be significantly more common in people with urinary tract infections. Arterial hypertension with heart failure was more often seen in patients with than in patients without proteinuria, but the difference was not statistically significan

ISSN:0304-324X    

DOI:10.1159/000213103

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

A retrospective study was made of 50 consecutive cases of bacteraemia treated in a geriatric unit. The mortality rate was 24%, the highest incidence occurring amongst the cases of hospital-acquired bacteraemia where only 1 of 7 cases survived the infection. Pyrexia, acute confusion, and falls were the commonest presenting symptoms. The most frequently identified tissue sources for the bacteraemia were the genito-urinary tract (24%) and the lungs (22%). In 20% of cases the source of infection was unidentified.

ISSN:0304-324X    

DOI:10.1159/000213104

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

3 cases of Werner’s syndrome were studied on serum lipid metabolism in comparison with clinical manifestation. These 3 cases showed hyperlipemia all with hypertriglycemia and increased level of very low density lipoprotein. The lipoprotein lipase activity was shown after intravenous injection of heparin. These results indicate a possible pathogenic facet of Werner’s syndrome from the lipid metabolic point of v

ISSN:0304-324X    

DOI:10.1159/000213105

出版商:S. Karger AG    

年代:1983

数据来源: Karger