ISSN:1660-8151    

DOI:10.1159/000185643

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Osteocalcin or bone Gla protein (BGP) is the most abundant noncollagenous protein of the skeleton. Serum BGP levels are thought to provide a valuable index of bone formation. We measured serum BGP and other parameters of mineral metabolism in 68 patients with functioning kidney grafts. The duration of the graft ranged from 1 to 131 months (mean 23). Serum BGP was positively correlated with parathyroid hormone (r = 0.56, p < 0.001). BGP was inversely correlated with glomerular filtration rate (r= –0.44, p < 0.001) and with the total cumulative dose of corticosteroids received after transplantation (r= –0.26, p < 0.05). No correlation was observed between BGP and 1,25(OH)2D, nor between BGP and serum aluminum. All patients with increased BGP in the presence of normal renal function had persistent hyperparathyroidism. The activity of the parathyroid glands and corticosteroid treatment seem to be the main pathophysiological factors influencing BGP levels after successful kidney graft

ISSN:1660-8151    

DOI:10.1159/000185644

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Serum Tamm-Horsfall protein (THP) levels were measured in 157 infants and children, 46 cord blood samples and 75 adults using an enzyme-linked immunosorbent assay. THP levels increased significantly across the age groups, suggesting a positive relationship with age. There was a strong positive relationship between serum THP and estimated glomerular filtration rate among children with chronic renal failure.

ISSN:1660-8151    

DOI:10.1159/000185645

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

The chronic hyperglycemia in diabetes mellitus enhances the nonenzymic glycation of structural proteins possibly increasing the formation of highly reactive advanced glycation end products (AGE). These protein changes might be involved in tissue-damaging mechanisms leading to diabetic complications, including diabetic nephropathy. To simulate these events, an in vitro model, based on isolated human glomerular basement membrane (hGBM), has been developed. In this study we have investigated the extent of AGE formation and the binding changes induced by the nonenzymic glycation of hGBM. An enriched fraction of hGBM was isolated from normal human kidneys and glycated in vitro by incubation with glucose (500 mmol/l) at 37 °C for 10 days. The presence of AGE was investigated by two methods – spectrofluores-cence and the diazonium salt reaction – both specific for this type of chemical entity. The binding capacity of glycated hGBM was tested by a 10-day incubation with human insulin, albumin, immunoglobulin G and fibrinogen. Higher relative spectrofluorescence values at 440 nm emission (20.0 ± 2.0 vs. 12.5 ± 5.0) and higher absorbance values at 492 nm (0.798 ± 0.063 vs. 0.429 ± 0.228) indicated the presence of increased levels of AGE in glycated vs. native hGBM. Insulin and the three proteins were bound to hGBM in increased amounts after its glycation (p < 0.05). The results obtained in this in vitro model confirm that enhanced nonenzymic glycation of hGBM induces the formation of AGE and possibly, through these compounds, alters its physicochemical and binding properties. This reation might contribute to the mechanisms eventually leading to diabetic nep

ISSN:1660-8151    

DOI:10.1159/000185646

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

In order to evaluate the antihypertensive effectiveness and interaction with ciclosporin A (CS-A) nitrendipine, a dihydropyridine derivative calcium entry blocking agent, was used in 16 (13 men, 3 women) hypertensive renal posttransplant patients followed by the Nephrology Department of Hacettepe University Hospital. The patients did not receive any antihypertensive drug for a 7-day period. They were then given 20 mg/day nitrendipine for 3 weeks. At the end of this period, mean ( ± SE) supine blood pressure fell from 163/108 ± 3.6/1.87 to 141/87 ± 3.8/2.2 mm Hg (p 0.05). We conclude that short-term therapy with nitrendipine in renal post-transplant patients does not appear to be harmful and longer term studies are needed to fully evaluate safety and efficacy of this drug. Because it influences neither blood chemistry nor renal functional parameters and blood CS-A level, it may be preferable to other calcium channel blocking agents in this group of pati

ISSN:1660-8151    

DOI:10.1159/000185647

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Advanced renal failure, nephrotic-range proteinuria due to proliferative glomerulonephritis and multiple myeloma with circulating IgG2λ and free λ light-chain paraproteins occurred in a 31-year-old male. Commonly established causes of renal failure in multiple myeloma were excluded. Immunofluorescence revealed heavy granular glomerular deposition of C3. Serum C3 was decreased, and C3c was increased. C3 nephritic-factor (C3 NeF)-like activity was demonstrated in the serum. Plasmapheresis and chemotherapy resulted in a decrease in paraprotein concentration up to 90%, a decrease in C3 NeF-like activity to negligible, normal serum complement levels and a marked improvement in both renal function and proteinuria. With reference to the literature, the possibility of a syndrome of paraproteinemia, C3 NeF-like activity and glomerulonephritis is forwarde

ISSN:1660-8151    

DOI:10.1159/000185648

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

The distribution of fibrin-related antigen (FRA) in glomeruli was examined by immunoelectron microscopy in 9 patients with idiopathic membranous nephropathy (MN), 8 patients with minimal-change nephrotic syndrome, and 10 patients with IgA nephropathy (IgA-N), using antisera against human γ-chain, α-chain, μ-chain, and fibrinogen. Electron-dense reaction products of FRA were observed in the endothelium, subendothelium, and/or in electron-dense deposits (EDD). Among the three glomerular diseases, the amount of electron-dense reaction products of FRA in the endothelium was highest in MN. This suggests that coagulation occurs on the endothelium in MN. Although the mesangial EDD of IgA-N were intensely stained with reaction products of FRA, the staining was weak in the subepithelial EDD of MN. This suggests that FRA hardly penetrates into the subepithelial EDD in

ISSN:1660-8151    

DOI:10.1159/000185649

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

We followed the renal and hormonal effects of physiological intravenous infusions of atrial natriuretic factor (ANF) in 6 water-loaded patients with nephrotic syndrome and 7 healthy subjects. Two of the patients had impaired renal function, 3 had active sodium retention, and none took drugs. The ensuing natriuresis, increase in plasma and urinary cyclic guanosine monophosphate and suppression of the renin-aldosterone axis were similar in normals and nephrotics. In both groups, significant increases in filtration fraction (inulin/PAH clearance) were observed, and in the nephrotics, major increases also occured in both the absolute and fractional urinary albumin excretion. The renal and hormonal responses to ANF are not impaired in the nephrotic syndrome.

ISSN:1660-8151    

DOI:10.1159/000185650

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Growth of the promyelocytic cell line HL60 and the erythroleukemia cell line K562 is inhibited by ‘uremic toxins’: creatinine, guanidino propionic acid and guanidino succinic acid in a concentration range similar to that of uremic sera. Among the tested compounds, creatinine exhibits the strongest and most dose-dependent inhibitory effect on both kinds of cells. These results provide a better understanding of the mechanism involved in the anemia of uremic patie

ISSN:1660-8151    

DOI:10.1159/000185651

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Serum aluminium was monitored in 19 patients admitted with acute oligo-anuric renal failure. The maximum serum aluminium obtained during the course of treatment was greater (p < 0.05) in 4 patients treated by haemodialysis alone, mean ± sem 3.78 ± 0.71 μmol/l than in 4 patients treated only by haemofiltration, 0.60 ± 0.22 μmol/l. For those 11 patients treated by both haemodialysis and haemofiltration, the maximum serum aluminium was greater (p < 0.05) during treatment with haemodialysis, 2.7 ± 0.62 μmol/l than during treatment with haemofiltration, 1.36 ± 0.15 μmol/l. There was a significant positive correlation between the maximum serum aluminium during treatment with haemodialysis and the number of hours of haemodialysis given (r = 0.76, p < 0.001). There was no significant increase in serum aluminium due to the administration of human albumin solutions. The aluminium content of dialysate water represents a major source of aluminium in patients with acute renal failure; prevention by reverse-osmosis water purification is rec

ISSN:1660-8151    

DOI:10.1159/000185652

出版商:S. Karger AG    

年代:1989

数据来源: Karger