摘要:

A total of 205 studies of hemodynamics and blood gas analyses were performed in relation to hepatic mitochondrial redox state in 23 partially hepatectomized patients. When the blood ketone body ratio (the ratio of acetoacetate to β-hydroxybutyrate in arterial blood) decreased, that is when the hepatic mitochondrial redox state was reduced, a hyperdynamic state with increased cardiac index, decreased systemic and pulmonary vascular resistance together with decreased oxygen consumption was observed in spite of the increased oxygen availability. Inhibited oxidative metabolism in the Krebs cycle may have resulted in the decreased oxygen consumption and decreased vascular tone. This study supports the concept that the hepatic metabolic derangements due to reduced hepatic mitochondrial redox state may be a factor in the decreased oxygen consumption and hyperdynamic state

ISSN:0014-312X    

DOI:10.1159/000129025

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Thromboxane, prostacyclin and their ratio could play an important role in the ischemic liver injury. To study this hypothesis, thromboxane and prostacyclin were measured by RIA after incubation of liver tissues removed during and after an ischemia of 90 min in male Wistar rats. The thromboxane to prostacyclin ratio increases during this period. In order to examine if this change could influence the survival rate of animals submitted to the same period of ischemia, drugs able to reduce the relative predominance of thromboxane were infused. The survival rate was not modified by administration of Iloprost or Daltroban, the antagonist of the thromboxane receptors. By contrast, imidazole, an inhibitor of thromboxane synthetase, significantly increased the survival rate. The same result was obtained with the administration of Daltroban plus Iloprost, suggesting that the reduction of thromboxane action associated with the increase of PGI2 level reduces the ischemic injury.

ISSN:0014-312X    

DOI:10.1159/000129026

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

The effect of a low-protein diet on the healing of colonic anastomoses in the rat was studied. The low-protein diet given for 2 weeks caused a weight loss of 11.9%. The healing of colonic anastomoses was assessed by bursting pressure measurement performed sequentially in the postoperative period. Compared to control animals, rats on a low-protein diet had significantly lower bursting pressures from the 4th to the 7th postoperative day. There was no difference in the hydroxyproline concentration at the anastomoses of the two groups of rats. These results suggest that the 5th postoperative day is the most appropriate time to investigate the effects of protein malnutrition on colonic healing in the rat.

ISSN:0014-312X    

DOI:10.1159/000129027

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Solid tumors contain hypoxic cells which are relatively resistant to antineo-plastic activity of radiation or to chemotherapeutic agents. We determined the increase in antineoplastic activities of mitomycin C (MMC) and carboquone (CQ) against human tumor cells under conditions of in vitro hypoxia, with the following results, (a) The HeLa cells had been exposed to various concentrations of MMC or CQ for 2 h under normally aerated (about 20%) or hypoxic conditions (5.0 and 0%) and were maintained under normally aerated conditions for 3 days. The succinate dehydrogenase activity was assayed, using the succinate dehydrogenase inhibiton (SDI) test. Treatment with MMC or CQ reduced the SD activity, compared to findings in the control cells, under hypoxic conditions, (b) The cells were exposed to the drug, MMC or CQ, under normally aerated or anoxic conditions, for 15–45 min and the colonies counted on day 14. Treatment with 0.45 µM of MMC for 45 min inhibited the clonogenicity of HeLa cells by 25.4% of findings in the control cells under anoxia. Treatment with 0.03 µM of CQ for 30 min inhibited the clonogenicity by 41.5%. (c) The sensitivity of 14 human tumor tissues (6 gastric and 8 colorectal cancers) to MMC or CQ under hypoxic conditions was determined, using the SDI test. MMC and CQ showed hypoxic enhancement. Therefore, MMC and CQ have definite positive effects on hypoxic cells, and hence a higher therapeutic ratio for treating clinical solid tum

ISSN:0014-312X    

DOI:10.1159/000129028

出版商:S. Karger AG    

年代:1989

数据来源: Karger

ISSN:0014-312X    

DOI:10.1159/000129029

出版商:S. Karger AG    

年代:1989

数据来源: Karger