摘要:

Sepsis score and complement factor B (FB) have been measured in 66 severely septic surgical patients in the intensive care unit, with the aim of monitoring their clinical course and predicting their outcome. Sepsis score correlated well with clinical course. 82% of patients with initial sepsis score < 20 progressively improved and survived. Only 6% of patients with sepsis score ≧ 20 survived. FB plasma level was significantly higher (p < 0.01) in patients who subsequently survived. Two indices were identified which could predict patient outcome several days in advance with 100% accuracy: (1) the index of survival from sepsis defined as the combination of sepsis score < 20 and FB ≧ 45 mg/dl, and (2) the index of death from sepsis defined as sepsis score ≧ 20 and FB < 40

ISSN:0014-312X    

DOI:10.1159/000128478

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Clearance of Escherichia coli from the bile was studied in 4 normal Sprague-Dawley rats and 4 rats with 3 weeks of obstruction of the common bile duct. I25I-radiolabelled heat-killed E. coli were injected into the common bile duct and the radioactivity of the bile monitored for 2 h. The radioactivity declined exponentially during the first 10 min. Bile samples collected from 2 to 15 min after injection showed higher amounts of radioactivity in all rats with biliary obstruction than in the normal rats. However, the clearance rate was higher in normal than in obstructed rats (p < 0.05). It was, therefore, concluded that the bacteria were cleared off the bile rapidly in normal rats by the function of a liver and/or a biliary tree. The present data, concerning the kinetic study of bacterial clearance from the biliary tract, indicates that the impaired clearance of bacteria in chronic biliary obstruction might be crucial for the development of biliary sepsis.

ISSN:0014-312X    

DOI:10.1159/000128479

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

The pancreatic blood flow and contrast enhancement in computed tomography (CT) were studied in 10 piglets weighing 17–25 kg with experimental pancreatitis. Each animal served as its own control. CT and blood flow measurements were made both before induction of pancreatitis and 5–6 h after the onset of the disease. Cardiac output was measured by thermodilution, and was kept constant throughout the study by infusion of dextran and saline. Although the pancreatic blood flow remained constant in this experiment, CT showed a significant decrease in contrast enhancem

ISSN:0014-312X    

DOI:10.1159/000128480

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

A method for the long-term diversion of bile in the rat is described. It was used to induce complete and uniform pancreatic exocrine atrophy, as confirmed by a marked loss of weight of the gland, very low pancreatic tissue amylase, and by histological examination. The technique induces changes in endocrinal function of the pancreas, since it increases pancreas insulin by a factor of 2.7, decreases glucagon by a factor of 0.19, and somatostatin by a factor of 0.6. The method was also used for collecting juice from the nonatrophied pancreas in the conscious rat, and may facilitate studies on pancreas physiology and pathophysiology.

ISSN:0014-312X    

DOI:10.1159/000128481

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

A perfluorocarbon emulsion, Fluosol-43, was used as a blood substitute for oxygen transport during isolation-perfusion of the dog liver at 37 and 43 °C. Preservation of hepatic functional integrity was assessed through analysis of perfusate constituents and animal survival after perfusion. Flow to the liver during perfusion was > 1 ml/min/g with one-third of total flow provided through the hepatic artery and two-thirds through the protal vein. Perfusion duration was 3 h. The pO2 gradient across the liver indicated that oxygen was consumed during perfusion at both temperatures. The expected rise in pCO2 and decrease in pH of the outflow perfusate is consistent with active aerobic metabolism. Perfusate chemistries lactate, pyruvate, glucose, urea, total α-amino acids, ketone bodies and SGPT demonstrated that hepatic functional integrity was maintained during perfusion. Significant differences (p < 0.05) between temperatures occurred in the perfusate levels of lactate, pyruvate, L/P ratios, glucose and total α-amino acids. Animal survival after a 3-hour perfusion was 3/4 at 37 °C, and 2/5 at 43 °C. After perfusion, SGPT levels were significantly higher in dogs subjected to perfusion at 43 °C. The success of these experiments demonstrates that perfusion of the liver with Fluosol-43 was not in itself hepatotoxic, and that Fluosol-43 may allow perfusion of the liver at 43 °C with only mild to

ISSN:0014-312X    

DOI:10.1159/000128482

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

An experimental study was conducted in rats to evaluate the sensitivity of the liver to infrared hyperthermia. A 15-min hyperthermia session treating only the liver was done in rats with a normal hepatic parenchyma and in rats with hepatocarcinoma induced by chronic 3’-diethylaminoazobenzene intoxication, at various ranges of intrahepatic temperature. In normal rats, 40–42 °C hyperthermia was well tolerated, but the mortality rate increased when the intrahepatic temperature exceeded 42 °C. In rats with tumors, a 40–42 °C hyperthermia session was well tolerated in case of small tumors, but resulted in a high mortality rate in case of large tumors. In all cases, death occurred as a consequence of liver injury. This study using a simple method of hyperthermia defines the thermosensitivity of the neoplastic or normal rat liver and provides a basis for further investigations on the effect of hyperthermia on experimental live

ISSN:0014-312X    

DOI:10.1159/000128483

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

The function of the ileocecal sphincter (ICS) in rabbits was investigated after extensive small bowel resection. The reflux pressure in anterograde (P1) and retrograde (P2) direction was measured before, 7 days after, and 1 year after small bowel resection, and compared with a group of rabbits in which a bypass-operation of the same length of the small bowel had been performed. After 7 days, both P1 and P2 showed a marked decrease in the resection group. One year after the operation, pressures had increased again but were still lower than before the resection. Significantly minor pressure changes were found 7 days after bypass surgery. We consider that ICS-insufficiency can be one cause of the features of short bowel syndrome.

ISSN:0014-312X    

DOI:10.1159/000128484

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Concentrations of several gastrointestinal hormonal peptides were measured in lymph from the cisterna chyli and in arterial plasma; in healthy, conscious pigs during ingestion of a meal. Lymph concentrations of the pancreatic hormones insulin, glucagon and pancreatic polypeptide were small compared with plasma concentrations, although postprandial increments were significant. In contrast lymph concentrations of gastrin, cholecystokinin, motilin and gastric inhibitory peptide (GIP) from the foregut showed a more marked postprandial rise than the pancreatic hormones. Indeed the total integrated responses of these peptides in lymph reached about 50% of those seen in arterial plasma. It would appear unlikely that the lymphatics constitute an important transport mechanism for these regulatory peptides. However, lymph concentrations of hormones may reflect levels in interstitial fluid better than plasma and may be of value in assessing putative physiological actions within a target tissue.

ISSN:0014-312X    

DOI:10.1159/000128485

出版商:S. Karger AG    

年代:1985

数据来源: Karger