ISSN:0014-312X    

DOI:10.1159/000129031

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Ciclosporin (CsA) was administered subcutaneously at a dose of 3 mg/kg body weight/day from the day of operation to 14 days of liver allotransplantation in ACI rat (RTla) to LEW rat (RT11) strain combination. All LEW recipients of ACI liver transplants without immunosuppressive treatment had severe rejection and expired within 12 days. In contrast, 7 out of 9 recipients in the same strain combination with temporary CsA treatment survived indefinitely. Histologically, widespread cellular infiltration and massive hepatocyte necrosis were evident upon autopsy of the recipients without CsA treatment. In contrast, in the surviving rats of the CsA-treated group, mononuclear cell infiltration was restricted to the periportal field and hepatocytes appeared to be normal at 14 days posttransplant. CsA concentrations in whole blood were determined by high-performance liquid chromatography. The trough levels were 788 ± 48, 621 ± 76 and 546 ± 52 ng/ml, at 5, 10 and 14 days posttransplant, respectively. We concluded that this relatively low-dose subcutaneous administration of CsA offered adequate immunosuppression in rat liver allotransplantation in this strain combinati

ISSN:0014-312X    

DOI:10.1159/000129032

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Four groups of isolated rat livers underwent perfusion at 37 °C for 1 h, at 37, 40, 43 or 45 °C, respectively, during the 2nd hour and then at 37 °C again for the 3rd hour. Vascular resistance slightly decreased during hyperthermia and then significantly increased after restarting normothermic perfusion. At 40 ° C bile production, oxygen consumption, glucose and lactate release did not significantly differ from those found in the 37 °C group. At 43 and 45 °C all these parameters were significantly impaired when compared to the 37 and 40 ° C groups and did not recover after normothermic perfusion was restored. GOT and GPT release increased in proportion to the temperature. Microscopic examination revealed normal histologic features in 37 and 40 °C specimens while alterations such as vacuolization and focal necrosis were found in the 43 and 45 °C groups. These data indicate that the highest temperature that is well tolerated by isolated rat livers for 1 h is located between 40

ISSN:0014-312X    

DOI:10.1159/000129033

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

In the present study, subtotal hepatectomy was evaluated as a model of acute liver failure in the rat. Sprague-Dawley rats, weighing 250–300 g, underwent hepatectomy under varying basal conditions of temperature and glucose administration. Rats operated and maintained postoperatively at ambient temperature (25 °C external environment) developed hypothermia with a rate of return to normal temperature which was related to the extent of hepatectomy and the availability of glucose postoperatively. However, no significant difference in survival was observed between groups maintained at ambient temperature and those whose core temperature was maintained at 37 °C by passive external warming. Severe hypoglycemia was observed in rats undergoing 90 and 95% hepatectomy without glucose postoperatively. With 20% glucose available in drinking water the mortality of 90% hepatectomy was reduced from 95 to 40% (p < 0.0001). With increase of the hepatectomy to 95%, 90% mortality was observed despite glucose support. Transplantation of 4 × 107 isolated syngeneic hepatocytes intraperitoneally at the time of hepatectomy did not increase survival after 90 or 95% hepatectomy; addition of testosterone therapy did not improve survival either alone or with hepatocyte transplantation. In this study, hepatectomy exceeding 90% was lethal and did not respond to the supportive measures provided. Hepatocyte transplantation and testosterone pretreatment, both therapies which are thought to increase regeneration, were ineffective in improving survival in this resection m

ISSN:0014-312X    

DOI:10.1159/000129034

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Pulmonary metastases of a weakly immunogenic methylcholanthrene-induced murine fibrosarcoma MCA-106 were treated on day 10 for 10 days with saline, interleukin-2 (IL-2), hybrid recombinant interferon A/D (rIFN A/D) or the combination. IL-2/IFN effected > 99% reduction in tumor compared to saline, IL-2 or IFN alone. However, IL-2/IFN also resulted in early ( 150 days). Interruption of the 10-day treatment with a 2-day rest at 5 days abrogated early mortality, but despite significant prolongation of survival, no long-term survivors were seen. These data suggest possible clinical application of IL-2/IFN in the treatment of advanced tumor metastases but cautions of increased treatment toxicity associated with this regimen.

ISSN:0014-312X    

DOI:10.1159/000129035

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

A peritoneal lavage model, cyclic intraperitoneal lavage (CIPL), and other adhesion preventing methods with and without fibrinolytic agents were compared to a control group without treatment in an animal study. The adhesion-preventing effect was evaluated at the site of a standardized peritoneal defect (free peritoneal grafting, P) and at the laparotomy wound (L) of 60 rats (12 escape) after surgical lysis of primary adhesions during relaparotomy. In five test groups with different treatments and in a control group without treatment recurrent adhesions were investigated during relaparotomy according to an adhesion grading scale with increasing severity (0-III). In the control group only severe adhesions grade II and III were observed. The five test groups showed different distributions of grade 0–II adhesions: compared to the control group a significant difference of the preventing effect was seen after CIPL with 1.36% glucose solution (as used for peritoneal dialysis) and after CIPL with Ringer’s solution at the sites P and L, after a one-time irrigation with Ringer’s solution only at the peritoneal graft P. Fibrinolytic agents used in CIPL or as single dose application failed to show an improvement compared to the control

ISSN:0014-312X    

DOI:10.1159/000129036

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Cardiopulmonary variables with intermittent positive pressure ventilation (IPPV) and high-frequency jet ventilation (HFJV) were compared in 8 patients undergoing elective abdominal aortic operations under fentanyl-nitrous oxide anesthesia. Hemodynamics were stable under the two methods of ventilation, and most of the corresponding nemo-dynamic variables were statistically indifferent from each other. Alveolar gas exchange was also adequate with either method and most of the corresponding respiratory variables were not statistically different. The peak airway pressure was significantly less during HFJV than during IPPV (p < 0.01). Diaphragmatic excursions are less during HFJV than during IPPV, resulting in a quieter surgical field. Therefore, hemodynamic stability and gas exchange adequacy coupled with less diaphragmatic excursions and lower peak airway pressure make HFJV an acceptable alternative to IPPV during abdominal aortic operations.

ISSN:0014-312X    

DOI:10.1159/000129037

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

The goal of this study was to evaluate the presence of extrahepatic damage and the uniformity and reversibility of the histological findings in CCl4.induced liver cirrhosis in the rat. To verify these findings rats were sacrificed 2 and 10 weeks after a treatment consisting of ten intragastric doses of CCl4, administered weekly. All treated rats developed an irreversible micronodular cirrhosis with no damage to the brain, kidney and pancreas. Moreover, rats sacrificed 2 weeks after the last CCl4 dose showed a number of functional alterations usually observed in man. In particular, low branched chain/aromatic amino acids (BCAA/AAA) plasma ratio, high ammonia, low zinc and high insulin with normal blood glucose were obtained.

ISSN:0014-312X    

DOI:10.1159/000129038

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

The endothelium plays an active role in inhibiting and promoting the activation of coagulation. Loss of the endothelial lining results in a thrombogenic state. The inactivation of thrombin on rabbit aorta after a superficial and deep injury to the vessel wall was studied. The thrombin enzymatic activity on the subendothelium could be modulated and decreased activity was found on both types of injured vessel segments when exposed to antithrombin III (AT). An increased activity by exposing the superficially injured vessels with heparin was seen. This effect of heparin was not observed on the deeply injured vessels. Most probably, heparin elutes AT from the surface of the superficially injured vessels, but on the deeply injured surface no AT is present and, consequently, heparin has no effect in this respect. These results speak in favour of treatment with AT instead of heparin in cases of arterial injury.

ISSN:0014-312X    

DOI:10.1159/000129039

出版商:S. Karger AG    

年代:1989

数据来源: Karger

ISSN:0014-312X    

DOI:10.1159/000129040

出版商:S. Karger AG    

年代:1989

数据来源: Karger