ISSN:0014-312X    

DOI:10.1159/000129067

出版商:S. Karger AG    

年代:1989

数据来源: Karger

ISSN:0014-312X    

DOI:10.1159/000315770

出版商:S. Karger AG    

年代:1989

数据来源: Karger

ISSN:0014-312X    

DOI:10.1159/000315771

出版商:S. Karger AG    

年代:1989

数据来源: Karger

ISSN:0014-312X    

DOI:10.1159/000315772

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

We have examined the immunosuppressive effects of combined azathioprine (Aza) and ciclosporin (CS) in two groups of mongrel dogs receiving kidney allografts. In group 1 Aza and CS were given together daily after transplantation and in group 2 twice the dose of each drug was given separately on alternate days. Doses were halved in each group at successive 84-day intervals and all immunosuppression was stopped on day 336. Thus the same total amounts of Aza and CS were given to all recipients in both groups. Up to day-60 the incidence of rejection in each group was similar, thereafter recipients in group 1 were more susceptible to fatal infection and marrow hypoplasia. This accounted for the difference in long-term survival between the two groups (1/14 in group 1, 5/12 in group 2 at day 420). Subsequently, two long-term survivors in group 2 died, 1 on day 452 from chronic rejection and the other on day 529 from gastroenteritis with a histologically normal allograft kidney. An in vitro analysis of the alloreactive repertoire of two healthy recipients in group 2, bearing each other’s kidneys for more than 2.5 years and more than 500 days without immunosuppression, showed a profound donor-specific defect which could account for their operationally tolerant stat

ISSN:0014-312X    

DOI:10.1159/000129005

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Searching for a marker of graft viability, adenosine triphosphate (ATP) content in the graft tissue was measured sequentially during orthotopic liver transplantation (OLT) in 24 adult mongrel dogs. According to the survival period, 12 recipients were devided into two groups: group I – early-death group (n = 6), death within 3 days after OLT and group II – survival group (n = 6), survived more than 7 days after OLT (average 12 days). Serum biochemical findings showed no differences between the two groups except that the prothrombin time on the first postoperative day in group I was significantly longer (p < 0.05). The ATP content ratio (% of initial value) was significantly higher in group II, compared to that of group I (p < 0.05), 94 versus 53% at 10 min after perfusion, 31 versus 17% at just before recirculation, respectively. The ATP content ratio recovered significantly to more than 80% at 10 min after recirculation in group II but only to less than 50% in group I. These results suggest that restoration of ATP content in the liver after graft recirculation reflects the viability of the graft and can serve as an appropriate prognostic marker in cases of

ISSN:0014-312X    

DOI:10.1159/000129006

出版商:S. Karger AG    

年代:1989

数据来源: Karger

ISSN:0014-312X    

DOI:10.1159/000315773

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Two methods of inducing liver cirrhosis in the rat were studied. Intragastric administration of CCl4 for 16 weeks according to Proctor and Chatamra was compared to the administration of thioacetamide in the drinking water (0.3 g/l) for the same period. CCl4 administration induced micronodular cirrhosis in 6/8 animals with a 27% mortality. Thioacetamide induced cirrhosis in 6/8 animals without mortality. The histologic pictures differed somewhat in that the CCl4 group exhibited more necrosis and cellular swelling while the thioacetamide group had more nuclear atypias and proliferation. Biochemically both groups had elevated plasma levels of aspartate aminotransferase. The lysosomal enzyme β-hexosaminidase (β-NAG) showed a transient increase in the thioacetamide animals, while β-glucuronidase decreased. CCU-induced cirrhosis led to an increase in β-NAG. Plasma zinc decreased in both groups as well as liver zinc content in the CCl4 group, while there was a continuous elevation of liver zinc in the thioacetamide group. We conclude that oral administration of thioacetamide is a simple and reliable method of inducing experimental liver cirrhosis. The differences in histological appearances and some biochemical parameters may be caused by the different mechanisms of action of thioacetamide and CCl4

ISSN:0014-312X    

DOI:10.1159/000129007

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

Papillary function in cholecystolithiasis patients was examined by intraoperative biliary manometry using a new balloon catheter. Pressure measurement was performed in each patient with the balloon deflated and inflated. When the balloon was inflated, the influence of the volume and compliance of the biliary tree was minimized to yield a precise evaluation of papillary function. From our analysis, resting pressure, residual pressure, onset time, and recovery time proved to be influenced by the volume and compliance of the biliary tree. Therefore, these four parameters could not reflect the papillary function exactly. On the other hand, perfusion pressure was not affected by the volume and compliance of the biliary tree. Perfusion pressure was thought to be generated by the resistance of the choledochal outlet and reflect the papillary function. In this study, our balloon catheter proved to be very useful in minimizing the influence of the volume and compliance of the biliary tree. And it is concluded that perfusion pressure is the best parameter to evaluate the resistance of the sphincter of Oddi or papillary function.

ISSN:0014-312X    

DOI:10.1159/000129008

出版商:S. Karger AG    

年代:1989

数据来源: Karger

摘要:

To examine the effects of portal blood deprivation on energy metabolism of the liver, we studied: (1) the ketone body ratio (acetoacetate/β-hydroxybutyrate) in liver tissue, which is in equilibrium with the free NAD+/NADH ratio in liver mitochondria, in the ligated lobe (LL) and nonligated lobe (NLL), (2) the hepatic energy charge [EC = (ATP + ½ADP)/(ATP + ADP + AMP)] in both LL and NLL, and (3) the arterial blood ketone body ratio (BKBR) after left portal vein branch ligation (PBL) in rabbits. As found in LL after PBL, portal blood deprivation decreased the tissue ketone body ratio. The EC in LL significantly decreased after PBL, but recovered 7 days after PBL since the LL became atrophic. The BKBR remained within the normal range, even when 60% of the total liver was deprived of portal bloo

ISSN:0014-312X    

DOI:10.1159/000129009

出版商:S. Karger AG    

年代:1989

数据来源: Karger