ISSN:0037-2366    

DOI:10.1080/00372367208057970

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

Zirconium phosphate is perhaps the first insoluble salt of a polybasic metal to be used as an ion exchanger. It is the most studied and probably the most useful of all the materials of this type. These materials are prepared by combining oxides of group IV with the more acidic oxides of groups V and VI of the periodic table, as shown in Table 1.

ISSN:0037-2366    

DOI:10.1080/00372367208057971

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

On the basis of both the literature data and the results of our own studies, some chemical and physicochemical aspects of selective flotation of inorganic ions was discussed. Classification of properties and events that determine the flotated ion separation, such as ionic charge, base-acid equilibria, complexation processes, solubility of ion-collector compounds, and ion affinity to surfactants have been described and exemplified.

ISSN:0037-2366    

DOI:10.1080/00372367208057972

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

The effect of drainage on the lesser accumulation ratio R in foam is calculated. If timet0alloted to drainage is varied, In R - 1) is expected to be, in simplest instances, proportional tot0. The expansion factor 1/ of the foam, when this reaches the receptacle, depends ont0but the product (R - 1) ought to be independent oft0These conclusions are not contradicted by the meager published data.

ISSN:0037-2366    

DOI:10.1080/00372367208057973

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

A modified random walk method, based on the Aris integrals, has been formulated for the evaluation of plate height contributions. The method is simple to apply, yet yields values in exact correspondence with that obtained from Giddings nonequilibium theory.

ISSN:0037-2366    

DOI:10.1080/00372367208057974

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

The following papers comprise the substance of a symposium on modern electrophoresis techniques, held at the Eastern Analytical Symposium in New York, November, 1971. The papers were chosen to illustrate the three main areas of development in this field: (1) electrophoresis theory, (2) advanced techniques, and (3) clinical applieations.

ISSN:0037-2366    

DOI:10.1080/00372367208057975

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

The field of polyacrylamide gel electrophoresis (PAGE) has undergone explosive development. Today it stands as one of the most popular methods for fractionation of proteins (and other charged macromolecules) and for testing homogeneity. The use of PAGE as a quantitative physical-chemical tool, for example, in the estimation of molecular weight, rather than as a qualitiative method, is already well established. Other applications, such as for measurement of free mobility, molecular net charge, isoelectric point, and apparent diffusion coefficient, are just beginning to be exploited. A recent article (1) and two monographs (2,3) review the progress in this field. However, before we can make further progress in this direction and evaluate the precision and accuracy of the results obtained, it is necessary to examine several basic assumptions, and ask

ISSN:0037-2366    

DOI:10.1080/00372367208057976

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

A method has been developed for estimation of the extent of polymerization of acrylamide by measurement of the amide-nitrogen of residual extractable acrylamide monomer. This method has been applied to the evaluation of polymerization efficiency, its reproducibility, and its dependence on the concentrations of three catalysts (persulfate, riboflavin, and TEMED) at pH 3.9 and 0°C. The effects of TEMED on gel pH, on the relative mobility of dyes and proteins, and on the “stacking limits” of multiphasic buffer systems have also been studied. These studies permit development of guidelines by which polymerization conditions can be optimized.

ISSN:0037-2366    

DOI:10.1080/00372367208057977

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

The properties of polyacrylamide gels with 1 to 50% cross-linking (%C) were studied using electrophoresis in multiphasic buffer systems of five proteins with known molecular weight. The extrapolated free mobility (Y0orM0varies in a biphasic manner with %C, reaching a maximum when %C is 5 to 7%, and falling to a constant plateau value specific for each protein when %C is greater than 20%.

ISSN:0037-2366    

DOI:10.1080/00372367208057978

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

The properties of a buffer system predicted by T. M. Jovin's theory of multiphasic (discontinuous) buffer systems have been tested experimentally both in free solution and in polyacrylamide gels. The most widely applied multiphasic buffer system, viz., the Tris-glycine system of Ornstein and Davis was used for this purpose. The properties of this system were computed on the basis of the Jovin theory. The pH, specific conductance, and boundary displacement were measured in the buffers corresponding to the original and the operative buffers of both the stacking (upper) gel and the separation (lower) gel in polyacrylamide gel electrophoresis. Values of pH and specific conductance in the absence of gels were found to be in reasonable agreement with theory. In polyacrylamide gels, after removal of residual reactants of the polymerization reaction by equilibration with the appropriate buffer, there was good agreement between observed and predicted values of pH and boundary displacement. However, the observed values for specific conductance were lower than predicted. The effects of pre-electrophoresis and of gel concentration on pH, conductance, and boundary displacement were also studied.

ISSN:0037-2366    

DOI:10.1080/00372367208057979

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor