摘要:

This case is of an unusual florid reactive CD4 + T Cell lymphocytosis involving lymph node (LN) and overshadowing residual B chronic lymphocytic leukemia (CLL). A 65 year old female with a 9 year history of untreated B-CLL presented with weight loss, splenomegaly and lymphadenopathy. B-CLL was confirmed on the basis of peripheral Blood lymphocytosis, bone marrow trephine findings and flow cytometry analysis. However, the LN biopsy showed appearances of a diffuse small lymphocytic population mimicking a leukemic T-cell infiltrate. Immunophenotyping and molecular analysis demonstrated the major cell population to be reactive CD4 positive T lymphocytes.

ISSN:1042-8194    

DOI:10.3109/10428199309148519

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

Two cell lines were originated from the peripheral Blood (PB-LAM) and bone-marrow (BM-LAM) of a patient with Burkitt-type acute lymphoblastic leukemia and AIDS. 26 and 7 clones were isolated from PB-LAM and BM-LAM respectively by limiting dilution. All of these had surface IgMλand the CD 10 marker with low to absent CD23, CD30, CD39 and surface adhesion molecules. Furthermore, they shared the same chromosomal abnormalities (trisomy 7 and t(8; 14) translocation) and the same rearrangements of immunoglobulin L and H chain and of c-myc gene loci. These features are those most frequently found in Burkitt's lymphoma (BL) cells and were different from those of the parental cell lines, which, besides cells identical to those of the malignant clones, also contained normal lymphoblastoid cells. Therefore, the cloning procedure used selected for the growth of cells with malignant features.EBV latent antigens were detected in all clones by Western blotting and their pattern of expression resembled that usually observed in BL cells. All the clones were positive for the EBV genome by Southern blotting and had monomorphic EBV-fused termini as determined by using cDNA probes specific for sequences at either end of the viral genome. However, the clones derived from PB-LAM had EBV fused termini of a different size from that of the clones derived from BM-LAM. The presence of different EBV-fused termini in otherwise monoclonal malignant cells indicate that EBV infection was possibly a late event in lymphomagenesis following rearrangement of the c-myc and the Ig gene loci.

ISSN:1042-8194    

DOI:10.3109/10428199309148520

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

An unusual case of CD4+helper T-cell lymphocytic leukaemia is reported in a 67-year-old Japanese woman. CD4+cells showed convoluted nuclei and dense cytoplasmic granules, features usually present in CD8+large granular lymphocytes and disorders of this particular cell type. Serum did not show antibodies to HTLV-I and HTLV-I proviral DNA integration was not evident by Southern blot analysis or after PCR. A monoclonal rearrangement of the TCR-βchain gene was evident when hybridization methods were used. The patient died 11 months after diagnosis. No skin involvement, or splenomegaly was evident. Serum LDH levels were markedly elevated but serum calcium levels were within normal limits. The case is discussed and compared to other T-cell lymphoid leukaemias. The heterogeneity in the morphology of CD4+T cell leukaemias is stressed.

ISSN:1042-8194    

DOI:10.3109/10428199309148521

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

Pure red cell aplasia was observed in a 28 year old woman following a major ABO mismatched allogeneic bone marrow transplant for chronic lymphocytic leukemia. No evidence of red Blood cell production was observed for more than one year following transplant despite the absence of high isohemagglutinin titers. Treatment with antithymocyte globulin resulted in prompt restoration of reticulocytosis. Therapy with antithymocyte globulin should be considered in these instances when red cell aplasia follows mismatched allogeneic marrow transplantation.

ISSN:1042-8194    

DOI:10.3109/10428199309148522

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

In 30 patients with multiple myeloma (MM) and mild to moderate anaemia (mean Hb 107 g/1, 95% confidence limit (CL) 102–113) but no evidence of renal failure (serum creatinine<110μmol/1), serum erythropoietin (EPO) showed significant inverse logarithmic correlation with the haemoglobin level (r=–0.57,p= 0.001). The observed/expected ratio of log-EPO in patients with MM (mean 0.96, CL 0.89–1.04) was similar to that of 119 subjects (mean 1.01, CL 0.96–1.05) with or without anaemia (mean Hb 116 g/L, CL 110–121) but without renal failure. The concentration of circulating erythroid progenitors (BFU-E) in 10 MM patients in plateau phase was significantly reduced (mean 0.70×105/l of Blood, CL 0.34–1.06) compared to that of 8 normal controls (mean 3.57, CL 1.60–5.55,p= 0.011)In vitrosensitivity of the BFU-E to EPO in the patients with MM was comparable to that of the normal controls. It appears that in MM there is an appropriate EPO response to anaemia but even in the plateau phase the number of circulating BFU-E is reduced, reflecting a degree of marrow failure. However, the progenitors are normally sensitive to EPO in such patients, and therapeutic doses of EPO may correct the anaemia by a pharmacological rather than a physiological effect.

ISSN:1042-8194    

DOI:10.3109/10428199309148523

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor