摘要:

AbstractThe radioactive melanin used in this study was extracted from the cell walls of the fungus Aspergillus nidulans after growth in the presence of14C‐labelled precursors. The counting systems investigated comprised solid samples suspended in a liquid scintillator with or without silica gel, solubilized samples blended in a liquid scintillator and solubilized samples adsorbed on to glass‐fibre discs wich were suspended in a liquid scintillator. Results obtained by counting solid samples with an end window detector are compared with the liquid scintillation data. High counting efficiencies were realized with the glass‐fibre disc method which is recommended for the assay of soluble samples. Liquid scintillation counting of silica gel suspensions was vastly superior to end window counting for the assay of solid samples. The data suggest that14C‐labelled materials of a particulate or highly pigmented nature can be counted with high efficiency in a liquid scintillation

ISSN:0022-2135    

DOI:10.1002/jlcr.2590040212

出版商:John Wiley&Sons, Ltd.    

年代:1968

数据来源: WILEY

摘要:

AbstractThe brown algae are characterised by their accumulation of polysaccharides, the more important ones being laminarin, fucoidin and alginic acid. It has been established by Bidwell et al.(1,2), that alginic acid is the most rapidly accumulated insoluble product on photosynthesis. The rapid appearance of such a large proportion of photosynthetic carbon in alginic acid suggests a possible method for the biosynthesis of radioactive alginic acid‐C‐14 (generally labelled, G). Such a synthesis is now carried out on laboratory scale and the details are given. The radioactive alginic acid‐C‐14(G) thus synthesised finds many uses, such as (1) for quantitative determination and estimation of alginic acid present in various seaweeds and (2) for ascertaining the role alginic acid plays in the metabolism of brow

ISSN:0022-2135    

DOI:10.1002/jlcr.2590040213

出版商:John Wiley&Sons, Ltd.    

年代:1968

数据来源: WILEY

ISSN:0022-2135    

DOI:10.1002/jlcr.2590040201

出版商:John Wiley&Sons, Ltd.    

年代:1968

数据来源: WILEY