摘要:

AbstractWe studied thein vitrophosphorylation pattern of the proteins fromin vivoirradiated isolated nuclei in relation to the investigations on rapid phytochrome effects onin vitrotranscription of specific genes in isolated nuclei from barley (Mösinger et al., 1985, 1987). Although phosphorylation with carrier‐free [γ‐32P]ATP raises a variety of labeled proteins, the overall pattern does not show any difference due to the light pretreatment of the nuclei. Time‐course analysis revealed a 17 and a 45 kDa protein as the most rapidly phosphorylated proteins. To obtain more information about the identity of both proteins we tried to determine the amino‐terminal sequences of both proteins. Whereas the 45 kDa protein has a blocked amino‐terminus, we identified the 17 kDa protein as H3 histone due to its sequence homology to other H3 histones from differ

ISSN:0932-8629    

DOI:10.1111/j.1438-8677.1991.tb00196.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

AbstractTransfer of the GUS gene into pollen has been studied in co‐cultures ofAgrobacteriumandPetuniapollen. TheAgrobacteriumstrain used contains a GUS gene between the two borders of the T‐DNA. Uptake and integration of this GUS gene could be shown using two different restriction systems. First, by appropriate cleavage within the T‐DNA the GUS gene could be isolated intact fromAgrobacterium‐treated pollen. Second, using enzymes with cleave the T‐DNA only once, integration of this T‐DNA into individual pollen genomes could be shown. The fragments obtained could not be obtained fromAgrobacteriumalone. The positive Southern blots were reprobed withvirprobes, but all were negative. Also following plating, noAgrobacteriacould be detected from our pollen DNA preparations. Therefore, the signals obtained were not due to contaminating bacteria. Due to a high endogenous GUS activity ofPetuniapollen the expression of the transferred gene could not be studied. The data demonstrate the uptake and integration of T‐DNA into pollen and are closing a gap in the line of evidence for the functioning of an indirectAgrobacterium— mediated gene transfer. Besides this, it should be stressed that only this indirect pollen system l

ISSN:0932-8629    

DOI:10.1111/j.1438-8677.1991.tb00197.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

AbstractFast protein liquid chromatography on Superose 6 of crude extracts fromChlorella kessleri, Fott et Novákóva, grown autotrophically in blue or in red light yields three different oligomeric forms of phosphofructokinase (PFK, EC 2.7.1.11). Their substrate affinities and responses to homotropic and heterotropic effectors are different.In vitro, the degree of oligomerization of the enzyme can be influenced by specific intermediates or cofactors. Its substrate, MgATP (10 mM/5 mM), and the negative effector, phosphoenolpyruvate (5 mM), both lead to some dissociation, while the second substrate, fructose‐6‐phosphate (5 mM), and the positive effector, inorganic phosphate (50 mM), have no effect. It is discussed whether formation or dissociation of oligomeric PFK formsin vivoresult from alterations in the levels or in the intracellular distribution of effector molecules and whether such processes are involved in the different regulation of cell metabolism in blue or in red

ISSN:0932-8629    

DOI:10.1111/j.1438-8677.1991.tb00198.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

AbstractA technique for the preparation of plasmodesmata within a cell wall fraction ofSolanum nigrumtissue homogenates has been developed, featuring good ultrastructural preservation of plasmodesmatal structure. SDS‐PAGE of protein extracts of this plasmodesmata‐containing cell wall fraction revealed the distinct enrichment of two bands, with molecular weights of 28 and 43

ISSN:0932-8629    

DOI:10.1111/j.1438-8677.1991.tb00199.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY