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21. |
Apamin-Sensitive K+Currents Mediate Arachidonic Acid-Induced Relaxations of Rabbit Aorta |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 413-419
Kathryn Gauthier,
Nancy Spitzbarth,
Erik Edwards,
William Campbell,
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摘要:
Abstract—Arachidonic acid induces an endothelium-dependent relaxation of the rabbit aorta that is blocked by lipoxygenase inhibitors. The cellular vasodilatory mechanisms activated by arachidonic acid metabolites remain undefined. In rabbit thoracic aortic rings pretreated with indomethacin (10 &mgr;mol/L) and contracted with phenylephrine, arachidonic acid (0.1 to 100 &mgr;mol/L) induced concentration-dependent relaxations. Maximal relaxations averaged 45±3% and were inhibited by increasing extracellular K+(30 mmol/L, 15±5%;P<0.001) or incubation with apamin (100 nmol/L, 26±7%;P<0.05) but not incubation with charybdotoxin (100 nmol/L, 41±5%). In aortic strips with an intact endothelium that were treated with phenylephrine, arachidonic acid (10 &mgr;mol/L) increased the membrane potential from −28.7±1.3 to −37.8±3.0 mV (P<0.01). Preincubation with apamin did not alter basal membrane potential but inhibited arachidonic acid-induced hyperpolarization (−31.5±1.5 mV). Incubation of rabbit aortic segments with apamin or charybdotoxin did not alter [14C]arachidonic acid metabolism. Whole-cell outward K+currents from isolated rabbit aortic smooth muscle cells averaged 43.0±4.8 pA/pF at 60 mV and were significantly decreased to 35.7±4.2 pA/pF by apamin (P<0.001). Subsequent addition of charybdotoxin further decreased maximal currents to 14.4±2.3 pA/pF. Addition of 11,12,15-trihydroxyeicosatrienoic acid increased the outward whole-cell K+current. In inside-out patches of aortic smooth muscle, apamin inhibited the calcium activation (100 to 300 nmol/L;P<0.001) of a small-conductance K+channel (≈24 pS). These results suggest that arachidonic acid induces endothelium-dependent hyperpolarization and relaxation of rabbit aorta through activation of smooth muscle, apamin-sensitive K+currents.
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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22. |
Decreased Endothelium-Dependent NO-cGMP Vascular Relaxation and Hypertension in Growth-Restricted Rats on a High-Salt Diet |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 420-427
Jason Payne,
Barbara Alexander,
Raouf Khalil,
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摘要:
Abstract—Low birth weight caused by placental insufficiency increases the risk of hypertension in young adults, particularly while ingesting a high-salt diet; however, the vascular mechanisms involved are unclear. We tested whether intrauterine fetal growth restriction results in salt-sensitive offspring that exhibit impaired endothelium-dependent relaxation, enhanced vascular contraction, and hypertension during high-salt diet feeding. Male offspring of control pregnant rats and pregnant rats with reduced uterine perfusion pressure (intrauterine growth restricted [IUGR]) were fed either a normal-sodium (NS, 1%) or a high-sodium (HS, 8%) diet. Body weight was less in IUGR/NS and IUGR/HS than in NS and HS rats. Arterial pressure was greater in IUGR/NS (144±4 mm|Hg) than in NS (131±3 mm|Hg) rats and far greater in IUGR/HS (171±12 mm|Hg) than in HS (129±2 mm|Hg) rats. In isolated, endothelium-intact aortic strips, phenylephrine (Phe, 10−5mol/L) caused an increase in active stress that was greater in IUGR/NS (13.9±0.9 N/m2) than in NS (8.5±0.6 N/m2) animals and far greater in IUGR/HS (18.2±1.2 N/m2) than in HS (9.4±0.8x104N/m2) rats. Acetylcholine caused relaxation of the Phe-mediated contraction and induced vascular nitrite/nitrate production that was less in IUGR/NS than in NS animals and far less in IUGR/HS than in HS rats.NG-nitro-l-arginine methyl ester, which inhibits nitric oxide (NO) synthase, or ODQ, which inhibits cGMP production in smooth muscle, inhibited acetylcholine relaxations and enhanced Phe contractions in NS and HS rats but not in IUGR/NS or IUGR/HS rats. Endothelium removal enhanced Phe-induced stress in NS and HS rats but not in IUGR/NS or IUGR/HS rats. Thus, endothelium-dependent relaxation via the NO-cGMP pathway is inhibited in systemic vessels of IUGR rats, particularly during intake of an HS diet. This might explain the increased vasoconstriction and arterial pressure in low-birth-weight offspring during ingestion of an HS diet.
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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23. |
Role of Platelet Microparticles in the Production of Thromboxane by Rabbit Pulmonary Artery |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 428-433
Sandra Pfister,
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摘要:
Abstract—This study examined the role of platelet microparticles in thromboxane A2(TXA2) production. Incubation of microparticles with [14C]arachidonic acid and A23187 produced14C-labeled TXB2, the stable metabolite of TXA2. To investigate the possibility that endothelial cells (ECs) transfer arachidonic acid to platelet microparticles and promote TXB2synthesis, ECs with their cellular lipids prelabeled with tritiated arachidonic acid were incubated with microparticles. In the absence of microparticles, there was no production of tritiated TXB2by the ECs. However, when microparticles were coincubated with prelabeled ECs, tritiated arachidonic acid was metabolized to tritiated TXB2. Aspirin was then used to inhibit cyclooxygenase. ECs coincubated with aspirin-treated platelet microparticles did not produce TXB2, as measured by radioimmunoassay. In contrast, aspirin-treated ECs coincubated with microparticles produced TXB2, and its production was enhanced by methacholine (10−4mol/L), indicating that endothelially derived arachidonic acid, and not endothelially derived prostaglandin endoperoxide, was transferred to the microparticle and further metabolized to TXA2. Additional studies with rabbit aorta and pulmonary artery investigated whether microparticles contributed to vascular contractions. Preincubation with microparticles enhanced arachidonic acid–induced contractions in the aorta and methacholine-induced contractions in the pulmonary artery. The thromboxane receptor antagonist SQ29548 and the thromboxane synthase inhibitor dazoxiben blocked these effects. Because TXA2is an important mediator in various pathophysiologic states, including hypertension, the ability of platelet microparticles to act as a cellular source of TXA2might provide new insight into the role of platelets and platelet microparticles in the control of vascular tone.
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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24. |
Reduced Endothelial NO-cGMP–Mediated Vascular Relaxation and Hypertension in IL-6–Infused Pregnant Rats |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 434-444
Julia Orshal,
Raouf Khalil,
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摘要:
Abstract—Placental ischemia during pregnancy is associated with increased plasma cytokines such as interleukin-6 (IL-6), which may contribute to increased vascular resistance and hypertension of pregnancy. We tested the hypothesis that an increase in plasma IL-6 during pregnancy is associated with impaired endothelium-dependent relaxation, enhanced vascular contraction, and hypertension. Systolic blood pressure was measured in virgin and pregnant Sprague-Dawley rats non-treated or infused with IL-6 (200 ng/kg per day for 5 days). Isometric contraction was measured in isolated aortic strips, and endothelial nitric oxide (NO) synthase (eNOS) was measured in aortic homogenate using Western blots. Blood pressure was greater in IL-6–infused (146±3) than in control pregnant rats (117±2 mm Hg). In endothelium-intact vascular strips, phenylephrine (Phe) caused greater increase in active stress in IL-6–infused (maximum: 10.6±0.6) than in control pregnant rats (maximum: 4.1±0.3×104N/m2). Acetylcholine (ACh)-induced relaxation of Phe contraction and vascular eNOS protein and nitrite/nitrate production were less in IL-6–infused than in control pregnant rats.N&ohgr;-nitro-l-arginine methyl ester (10−4mol/L), inhibitor of NOS, or 1H-[1,2,4]oxadiazolo[4,3]-quinoxalin-1-one (10−5mol/L), inhibitor of cGMP production in smooth muscle, inhibited ACh-induced relaxation and enhanced Phe-induced stress in control but not IL-6–infused pregnant rats. Endothelium removal enhanced Phe-induced stress in control but not in IL-6–infused pregnant rats. The blood pressure and vascular Phe-induced contraction, ACh relaxation, and eNOS protein were not different between control and IL-6–infused virgin rats. Thus, an endothelium-dependent NO-cGMP–mediated relaxation pathway is inhibited in systemic vessels of pregnant rats infused with IL-6. The results support a role for IL-6 as a possible mediator of the increased vascular resistance during hypertension of pregnancy.
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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25. |
Modifier Locus on Mouse Chromosome 3 for Renal Vascular Pathology in AT1AReceptor-Deficiency |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 445-451
Thu Le,
Agnes Fogo,
Harmony Salzler,
Tania Vinogradova,
Michael Oliverio,
Douglas Marchuk,
Thomas Coffman,
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摘要:
Abstract—We previously showed that the phenotype of mice with targeted disruption of the gene encoding the AT1Areceptor (Agtr1a), the major murine AT1receptor isoform, is strongly influenced by recessive genetic modifiers derived from the C57BL/6 or 129 inbred strains. To further evaluate the genetic modifiers on the C57BL/6 background, we performed backcrosses between F1(C57BL/6×129) and C57BL/6Agtr1a−/−mice and analyzed the progeny, focusing on the development of structural lesions in the renal vasculature. In affected animals, these lesions are characterized by medial thickening of small arteries and arterioles in the kidney that are reminiscent of vascular lesions in patients with nephrosclerosis. Among 180 consecutive progeny, 170 (94%) survived to completion of the study. On masked pathological examination at age 8 months, 86 had intermediate to severe vascular lesions whereas 84 had no detectable lesions. Based on a hypothetical model of a single recessive modifier locus arising from the C57BL/6 background, the observed proportion of affected animals among the backcross progeny was not statistically different from that predicted by &khgr;2analysis (51% versus 50%;P=0.88). We next performed genomic microsatellite analysis in a subset of 121 backcross progeny using a panel of markers spanning ≈15 cM intervals across the mouse genome. By 2-point analysis, we found a region spanning 5 cM on chromosome 3, with significant linkage to the development of renal vascular lesions (LOD score: 3.3 to 3.8).
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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26. |
Kallikrein Gene Transfer Protects Against Ischemic Stroke by Promoting Glial Cell Migration and Inhibiting Apoptosis |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 452-459
Chun-Fang Xia,
Hang Yin,
Cesar Borlongan,
Lee Chao,
Julie Chao,
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摘要:
Abstract—Kallikrein/kinin has been shown to protect against ischemia/reperfusion-induced myocardial infarction and apoptosis. In the present study, we examined the potential neuroprotective action of kallikrein gene transfer in cerebral ischemia. Adult, male Sprague-Dawley rats were subjected to a 1-hour occlusion of the middle cerebral artery followed by intracerebroventricular injection of adenovirus harboring either the human tissue kallikrein gene or the luciferase gene. Kallikrein gene transfer significantly reduced ischemia-induced locomotor deficit scores and cerebral infarction after cerebral ischemia injury. Expression of recombinant human tissue kallikrein was identified and localized in monocytes/macrophages of rat ischemic brain by double immunostaining. Morphological analyses showed that kallikrein gene transfer enhanced the survival and migration of glial cells into the ischemic penumbra and core, as identified by immunostaining with glial fibrillary acidic protein. Cerebral ischemia markedly increased apoptotic cells, and kallikrein gene delivery reduced apoptosis to near-normal levels as seen in sham control rats. In primary cultured glial cells, kinin stimulated cell migration but inhibited hypoxia/reoxygenation-induced apoptosis in a dose-dependent manner. The effects of kinin on both migration and apoptosis were abolished by icatibant, a bradykinin B2receptor antagonist. Enhanced cell survival after kallikrein gene transfer occurred in conjunction with markedly increased cerebral nitric oxide levels and phospho-Akt and Bcl-2 levels but reduced caspase-3 activation, NAD(P)H oxidase activity, and superoxide production. These results indicate that kallikrein gene transfer provides neuroprotection against cerebral ischemia injury by enhancing glial cell survival and migration and inhibiting apoptosis through suppression of oxidative stress and activation of the Akt–Bcl-2 signaling pathway.
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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27. |
Forced Homodimerization by Site-Directed Mutagenesis Alters Guanylyl Cyclase Activity of Natriuretic Peptide Receptor B |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 460-465
Thomas Langenickel,
Jens Buttgereit,
Ines Pagel,
Rainer Dietz,
Roland Willenbrock,
Michael Bader,
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摘要:
Abstract—Natriuretic peptides mediate their physiologic effects through activation of membrane-bound, guanylyl cyclase–coupled receptors (NPRs). Receptor dimerization is an important feature of signal transduction. This study was aimed at characterizing structurally important residues of the extracellular ligand-binding domain of NPR-B for receptor dimerization and cGMP generation. Deletion mutagenesis was used to replace cysteine residues at positions 53 (C53S), 417 (C417S), and 426 (C426S) by serine. Receptor expression, dimerization, whole-cell cGMP response, and guanylyl cyclase activity of membrane fractions were determined in stably transfected COS-7 cells. C53S, C417S, and C426S mutants were expressed and found to form disulfide-bridged covalent dimers. In contrast to NPR-B and C53S, C417S and C426S mutants displayed constitutive activity in whole cells (C417S, 146±12%,P<0.01; C426S, 153±7% of ligand-independent NPR-B cGMP generation,P<0.01). The cGMP response of C417S and C426S mutants in whole cells was dose dependent and ≈4 times lower than that in NPR-B, whereas it was blunted in C53S-transfected cells (1 &mgr;mol/L CNP, NPR-B 2868±436%; C53S, 206±16% of control,P<0.001 vs NPR-B, C417S, and C426S). Guanylyl cyclase assay in transfected cells confirmed the constitutive activity of C417S and C426S mutants. These data suggest that receptor dimerization by covalent disulfide bridges alters ligand-independent as well as ligand-dependent receptor activity. Localization of the crosslink in relation to the cell membrane is important for configuration of the extracellular domain and the consecutive signal transduction.
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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28. |
Two Quantitative Trait Loci Affect ACE Activities in Mexican-Americans |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 466-470
Candace Kammerer,
Nicolas Gouin,
Paul Samollow,
Jane VandeBerg,
James Hixson,
Shelley Cole,
Jean MacCluer,
Larry Atwood,
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摘要:
Abstract—Angiotensin-converting enzyme (ACE) activity is highly heritable and has been associated with cardiovascular disease. We are studying the effects of genes and environmental factors on hypertension and related phenotypes, such as ACE activity, in Mexican-American families. In the current study, we performed multipoint linkage analysis to search for quantitative trait loci (QTLs) that affect ACE activities on data from 793 individuals from 29 pedigrees from the San Antonio Family Heart Study. As expected, we obtained strong evidence (maximum log of the odds [LOD]=4.57, genomicP=0.003) that a QTL for ACE activity is located on chromosome 17 near theACEstructural locus. We subsequently performed linkage analyses conditional on the effect of this QTL and obtained strong evidence (LOD=3.34) for a second QTL on chromosome 4 near D4S1548. We next incorporated theACEIns/Delgenotypes in our analyses and removed the evidence for the chromosome 17 QTL (maximum LOD=0.60); however, we retained our evidence for the QTL on chromosome 4q. We conclude that the QTL on chromosome 17 is tightly linked toACEand is in strong disequilibrium with the insertion/deletion polymorphism, which is consistent with other reports. We also have evidence that an additional QTL affects ACE activity. Identification of this additional QTL might lead to alternate means of prophylaxis.
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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29. |
Autosomal-Dominant Hypertension With Type E Brachydactyly Is Caused by Rearrangement on the Short Arm of Chromosome 12 |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 471-476
Sylvia Bähring,
Anita Rauch,
Okan Toka,
Christoph Schroeder,
Christiane Hesse,
Heike Siedler,
Gabor Fesüs,
Walter Haefeli,
Andreas Busjahn,
Atakan Aydin,
Yvette Neuenfeld,
Astrid Mühl,
Hakan Toka,
Maik Gollasch,
Jens Jordan,
Friedrich Luft,
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摘要:
Abstract—We are studying a Turkish family with autosomal-dominant hypertension and brachydactyly; affected persons die of stroke before 50 years of age. With interphase fluorescence in situ hybridization, we found a chromosome 12p deletion, reinsertion, and inversion in affected persons. This finding suggested that the hypertension could be caused by one or more of 3 genes, the ATP-dependent potassium channel Kir6.1, its regulator the sulfonyl urea receptor SUR2, and the phosphodiesterase PDE3A. We further studied 6 affected and 4 nonaffected persons. Buttocks biopsies were done, small vessels were tested on a myograph, and mRNA was extracted. We performed forearm blood flow studies with intrabrachial artery diazoxide, isoproterenol, and milrinone infusions. Systemic pharmacological testing was done with intravenous diazoxide, nitroprusside, and isoproterenol. PDE3A mRNA was high in vessels from 3 affected subjects, but not high in 3 others. The vessels responded similarly to forskolin, with or without glibenclamide, and to cromakalim. However, there was a suggestion that the dilatation after milrinone might be exaggerated. The forearm infusion studies showed no differences in the responses to diazoxide, isoproterenol, or milrinone. Systemically, affected persons showed a greater blood pressure response to diazoxide and nitroprusside, and a greater heart rate response to isoproterenol than nonaffected persons. The results shed doubt on Kir6.1 and SUR2. The differences in PDE3A expression and responses may be the result of hypertension rather than the cause. Although our 3 candidate genes are no longer likely, the rearrangement we describe greatly enhances the perspectives of this project.
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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30. |
Positional Identification of Hypertension Susceptibility Genes on Chromosome 2 |
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Hypertension: Journal of The American Heart Association,
Volume 43,
Issue 2, Part 2,
2004,
Page 477-482
Ruth Barkley,
Aravinda Chakravarti,
Richard Cooper,
R. Ellison,
Steven Hunt,
Michael Province,
Stephen Turner,
Alan Weder,
Eric Boerwinkle,
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摘要:
Abstract—Chromosome 2 has been consistently identified as a genomic region with genetic linkage evidence suggesting that one or more loci contributes to blood pressure and hypertension status. As with all complex disease traits, following-up linkage evidence to identify the underlying susceptibility gene(s) is an arduous yet biologically and clinically important task. Using combined positional candidate gene methods, the Family Blood Pressure Program (FBPP) has concentrated efforts in narrowing a large region of chromosome 2, demonstrating evidence for linkage in several populations, and identifying underlying candidate hypertension susceptibility gene(s). Initial informatics efforts identified the boundaries of the region and the known genes within it. A total of 82 polymorphic sites in 8 genes were genotyped in a large hypothesis-generating sample consisting of 1640 African Americans, 1339 whites, and 1616 Mexican Americans. After resampling-based false discovery adjustment,SLC4A5, a sodium bicarbonate transporter, was identified as a primary candidate gene for hypertension. Polymorphisms inSLC4A5were subsequently genotyped and analyzed for validation in two other subcomponents of the FBPP, each contributing African Americans (N=461; N=778) and whites (N=550; N=967). Again, single nucleotide polymorphisms within this gene were significantly associated with blood pressure levels and hypertension status. Although not identifying a single causal gene variant that is significantly associated with blood pressure levels and hypertension status across all samples, the results further implicateSLC4A5as a candidate hypertension susceptibility gene. Moreover, the present study validates previous evidence for one or more genes on chromosome 2 that influence hypertension-related phenotypes in the population-at-large.
ISSN:0194-911X
出版商:OVID
年代:2004
数据来源: OVID
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