摘要:

AbstractWe have previously described the procedure for quantitative separation of extracellular and intracellular ChEs using mild treatment of rat superior cervical ganglion with papain. Here, this procedure was used in order to investigate the recovery of ChEs in the two pools after irreversible inhibition by soman which was directly injected into the ganglion. After such treatment only ganglion ChEs were totally inhibited, whereas the activity of ChEs in preganglionic neurons and their axons remained unaffected. Comparing in innervated and decentralized ganglia the pattern of recovery rate and ultrastructural reappearance of ChEs after local inhibition, it was possibleto distinguish between the indirect effects of innervation on the recovery rate and pattern of ChEs of ganglion origin and the direct contribution to the total ganglion enzyme activity of ChEs originating in the preganglionic elements.The absence of nerve contracts affects mostly extracellular activity, particularly AChE, whereas the intracellular activity of AChE was only slightly decreased and the activity of nsChE was somewhat increased. This increase coincides with the enhanced cytochemical reaction of nsChE in some nonneuronal cells in the ganglion.Actinomycyn D decreased the rate of initial rapid phase of recovery of intracellular ChEs when injected in the ganglion daily for three days, whereas the recovery of extracellular ChEs was already decreased the first day of Actinomycyn D application. This indicates that the externalization of the enzyme is more affected than its synthesis by inhibition the translation step.The results further suggest that functional innervation of the ganglion down‐regulate the rate of reappearance of the new enzyme at the extracellular and intracellular locations and changes the ratio between AChE and nsChE. ChEs synthesized in preganglionic neurons begins to contribute directly to the total ganglion ChEs activity in the late stages of enzyme recovery in spite of the presence of the preganglionic ChEs which was not inhibited after intraganglionic injection of soman. This finding suggests that the externalization and extracellular attachment of ChEs and not their preganglionic resynthesis may be the cause for delayed reappearance of ChEs affiliated with nerve terminals in the ganglio

ISSN:0360-4012    

DOI:10.1002/jnr.490170112

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

ISSN:0360-4012    

DOI:10.1002/jnr.490170113

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

ISSN:0360-4012    

DOI:10.1002/jnr.490170114

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

ISSN:0360-4012    

DOI:10.1002/jnr.490170115

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

ISSN:0360-4012    

DOI:10.1002/jnr.490170116

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

ISSN:0360-4012    

DOI:10.1002/jnr.490170117

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

ISSN:0360-4012    

DOI:10.1002/jnr.490170101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY