摘要:

specificity of IgE binding to a human basophil‐like cell line (KU812) was studied by flow cytometry. Four IgE myeloma proteins, representing both light‐chain types, one chimeric IgE protein, and polyclonal serum IgE blocked the direct binding of FITC‐labeled IgE(DES) myeloma protein to KU812 cells in a dose‐dependent and nearly equimolar way. Although not as efficiently as human IgE (from five to eight times less on a molar basis), both rat and mouse IgE blocked IgE(DES)‐FITC binding to KU812 cells. In sharp contrast, all four human IgG subclasses, both IgA subclasses, and IgM myeloma proteins, as well as monomeric and heat‐aggregated polyclonal human IgG, were unable to block significantly IgE(DES)‐FITC binding to KU812 cells (≤0.5/0 on a molar basis). The cytophilic epitope on IgE was heat‐susceptible (56 °h), lost after reduction alkylation, and resident in the papain‐derived Fcɛ‐fragment, but not in the papain‐derived F(ab')2ɛ.‐ and Fcɛ‐fragments nor in the pepsin‐derived F(ab')2ɛ‐ and Fcɛ.'‐fragments. Washing and displacement experiments indicated that a major part of IgE reacted with high affinity to KU812 cells. The results indicate that the binding of IgE to KU812 cells is highly specific and involves the classical high‐affinity FcɛRI‐receptor. Although the density of receptors is low, this human cell line offers a

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1995.tb02485.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

There are several reasons for considering the hypothesis that food hypersensitivity might relate to rheumatic diseases.•Food can evoke immune responses.•Food can cause immunologically mediated symptoms.•Immunological mechanisms are important in the pathogenesis of rheumatic diseases.•Antigens triggering rheumatic responses are unknown.•Rheumatic diseases have been associated with foods in man

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1995.tb02727.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1995.tb04352.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

•Specific chemical mediator release such as histamine and the prostaglandins (PG2a or PGD2) associated with headaches has been found in a few patients who were repeatedly challenged with specific foods, using DBPCFC technique

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1995.tb02728.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1995.tb04353.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

A 29‐year‐old female bakery shop assistant was occupationally sensitized to flour allergens andAspergillusα‐amylase (Asp o2). The latter represents a strongly allergenic component of routinely used baking additives. The patient had repeatedly responded to the consumption of white bread with rhinitis, conjunctivitis, and, occasionally, wheal and flare reactions. She underwent allergologic investigations including oral challenge tests with commercially available bread loaves. Elevated specific IgE antibodies against bread extracts,Asp o2, and flour allergens were detectable in her serum. The provocation test with bread resulted in a running nose together with a strong increase in nasal resistance. All symptoms subsided about 3 h after the challenge. None of the above symptoms could be observed when bread free ofAspergillusα‐amylase was administered. This outcome provides evidence of a clinically relevant persistent allergenicity toAsp o2

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1995.tb02487.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Levels of soluble intercellular adhesion molecule‐1 (sICAM‐1) were measured in sera from patients with an acute exacerbation of their atopic dermatitis (AD) (n= 16) on admission to and discharge from our department of dermatology. At admission, the sICAM‐1 levels in sera from patients with AD were slightly higher than those of the blood donors (n= 100) and dropped at discharge significantly (P= 0.014) after improvement of the skin conditions. Therefore, sICAM‐1 may be, together with soluble interleukin‐2 receptor (sIL‐2), eosinophilic cationic protein (ECP), and CD14, another marker for mo

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1995.tb02488.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

In the course of analyzing the partial amino acid sequences ofCry jI, a major allergen of Japanese cedar(Cryptomeria japonica)pollen, we found a peptide fragment which has a significant homology to some pectate lyase isozymes secreted by plant pathogenic bacteria. Therefore, we investigated whetherCry jI has pectate lyase activity.Cry jI reacted with polygalacturonic acid, resulting in the release of unsaturated uronide products. The optimum temperature and pH for the reaction were 60–70°C and pH 10. The enzymatic reaction had an absolute Ca2+ion requirement. These characteristics were very compatible with the character of the pectate lyase isozymes reported previously. These results clearly show thatCry jI has pectate lyase activi

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1995.tb02489.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY