ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04167.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Fourteen children with timothy grass pollinosis were given immunotherapy (IT) for 3 years with a purified and characterized timothy grass pollen preparation or a crude aqueous timothy pollen extract. Crossed radioimmunoelectrophoresis (CRIE) showed that 75% of the children under 11 years of age developed new specificities of IgE antibodies against timothy antigens, in contrast to older children, where no development of IgE antibodies against new timothy antigens could be detected. IgE antibodies were only detected against antigens formerly known as allergens. Timothy‐specific IgG antibodies increased in most children during hyposensitization against the major allergens Ag 19 and Ag 24/25 and several other IgE‐binding timothy antig

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04168.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

IgE and IgG antibody responses to birch pollen antigens were studied by means of immunoblotting experiments testing 58 sera from patients with Type I allergy to birch pollen. 56/58 patients showed IgE antibodies reactive with Bet v I, a 17 kilodalton (kD) pollen protein. 2D‐electrophoresis/immunoblot revealed a heterogeneity of that protein. Ten spots (pH 4.9–5.9) could be detected, presumably representing differentially glycosylated isoallergens. In 33/58 patients, there was no evidence of IgE antibodies directed against allergens other than Bet v I. However, in 25/58 of patients' sera, 11 minor allergens (13, 15, 18, 27, 29, 32, 36, 39, 44, 57, and 68 kD) with individual incidences from 1.7% to 17.2%, were identified. All proteins were also recognized by the patients' IgG antibodies: in the case of Bet v I recognition was weak, whereas the IgG response to the minor allergens was pronounced. Sera from healthy individuals showed similar IgG antibody responses, but no IgG to the 15, 27, and 29 kD proteins. Our results suggest that IgG directed against minor allergens may function as trapping antibodies in healthy individuals. Too low or lacking amounts of anti‐Bet v I IgG may facilitate an allergic rea

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04169.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The study was undertaken to evaluate the effect washing at various temperatures would have on the house dust mite,Dermatophagoides pteronyssinus, and its allergens. Young and old mite cultures underwent a simulated washing programme. To record the number of live mites prior to and after washing a new counting method was applied. The findings demonstrated that although washing reduced the number of live mites, a washing programme at minimum temperature 58°C was required in order to achieve complete extermination of the house dust mites. A distinct protein loss was observed when washing at higher temperature, thus further reducing the total allergen pool without loss of allergen activity as determined by direct RAST

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04170.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Dust samples were collected twice from smooth and carpeted floors in 10 Norwegian schools. The content of antigens and allergens of alder(Alnus incana), birch(Betula verrucosa), timothy(Phleum pratense), cat and dog dander, house dust mite(Dermatophagoides farinae), mould(Cladosporium herbarum), hen egg white and codfish(DIII)were investigated by crossed immunoelectrophoresis (CIE), crossed radio immunoelectrophoresis (CRIE), radio allergosorbent test (RAST) inhibition and quantitative precipitation inhibition analysis by laser nephelometry. Antigens and allergens of cat and dog dander and hen egg white were most prevalent in the dust samples investigated. With the exception of hen egg white and codfish allergens, no statistically significant differences in mean allergen content were shown in identical quantities of freeze‐dried dust extracts from carpeted and smooth floors. RAST‐inhibition analyses of identical amounts of dust from either floors showed higher content of allergens of cat, dog, hen egg white, codfish, mould and timothy pollen in classrooms with carp

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04171.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Sixty‐seven patients with a history of severe systemic reactions following honey bee stings were treated by immunotherapy (IT) with honey bee venom. During maintenance therapy all were submitted to a sting challenge under clinical conditions. 15 developed mostly minor symptoms of a systemic reaction while 52 showed only a local swelling at the sting site. Phospholipase A2‐specific IgE, IgG and IgG subclass serum antibodies were estimated in samples obtained before IT and immediately before the challenge. Specific IgE decreased in reactors and in non‐reactors. There was no difference between the two groups at any time. Specific total IgG, IgG1and IgG4increased in both reactors and non‐reactors during IT. An early increase of specific IgG1, was observed while specific IgG4remained elevated throughout the treatment. Specific total IgG was higher in reactors than non‐reactors before the challenge, specific IgG1higher in reactors before treatment and specific IgG4higher in reactors than non‐reactors both before treatment and before challenge. In the individual patient, no single antibody estimation or combination of various antibodies was predictive of the outcome of a stin

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04172.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We describe the assay conditions for an enzyme‐linked immunoassay for the determination of IgG and IgG subclass antibodies in serum to water‐soluble wheat flour antigens. The optimal antigen coating concentration was 5 μg/ml for total IgG, IgG1, IgG4and 100 μg/ml for IgG2. Serial dilutions of test sera were used and commercially available monoclonal mouse anti‐human IgG isotype antibodies (as ascites fluid) were diluted 1:500–1:1000. Specific wheat flour antibodies belonging to the IgG1, IgG2and IgG4subclasses were detected. Despite the lack of standardized isotype‐specific second mouse monoclonal antibodies, the subclass antibody levels between flour‐exposed bakers and controls could be compared. We observed significantly higher IgG1, IgG2and IgG4subclass antibodies among 23 bakers than among 12 non‐exposed controls, but no IgG3antibodies were detected. The differences in biological activities of the IgG subclass antibodies may explain the clinical and pathophysiological features for flour‐induced occupational allergic dise

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04173.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The aim of the study was to determine whether IgG antibodies from patients on specific immunotherapy could inhibit skin test reactivity of allergens when mixed with them prior to testing. In a preliminary double‐blind study, mite extracts were incubated with glycero‐saline solution or non‐specific IgG and used in testing 15 patients. Wheal and flare diameters produced with the two mite extracts did not differ significantly. In a second double‐blind study with 15 other patients, skin tests using mite extracts incubated with either non‐specific IgG or specific IgG showed significantly decreased diameters when mite extracts were preincubated with specific IgG. This result,in vivo, confirms the antigen neutralizing capacity of specific IgG demonstrate

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04174.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The aim of this study was to evaluate the effect of levocabastine, a new H1‐blocking antihistamine for topical use, in comparison with sodium cromoglycate on conjunctival symptoms of birch pollinosis. The two drugs were compared in a randomized double‐blind comparative study over 5 weeks in 37 children and adolescents (6–19 years of age) with birch pollen conjuncitivitis. Nasal symptoms occurred in 31 of the children and were treated with beclomethasone dipropionate nasal spray. An oral antihistamine was offered as rescue medication for eye symptoms. Initially, the patients received placebo four times a day for a 7‐day run‐in period. Conjunctival symptoms were recorded daily on diary cards on a 100 mm visual analogue scale. The pollen counts indicated a short but intensive birch pollen season. There was no statistically significant difference between the two treatment groups with regard to eye symptom scores before and during active treatment. However, the patients' evaluation of the efficacy of the therapy was in favour of levocabastine (P<0.01). Topical levocabastine, an H1‐blocker, applied twice daily, seems to protect from symptoms of allergic conjunctivities as favourably as sodium cromoglycate applied four times a day. There was no difference in number or character of reported adverse reactions between the two treat

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04175.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The safety and efficacy of loratadine was compared with that of dexchlorpheniramine in children with allergic rhinitis. Twenty‐one children received loratadine 0.11–0.24 mg/kg ideal body weight once daily and 19 dexchlorpheniramine 0.10–0.23 mg/kg every 8 h (0.30–0.69 mg/24 h) for 14 consecutive days. Both loratadine and dexchlorpheniramine were effective in reducing nasal and ocular symptoms in allergic children. Substantial improvement in allergy symptoms was observed at the first evaluation (day 3 of treatment) and was maintained for the study duration. No significant trend of abnormality in laboratory parameters was observed. Drowsiness was present only in the dexchlorpheniramine‐treated group. Loratadine appears to be a simple, effective and safe therapy for seasonal allergic

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1989.tb04176.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY