摘要:

The content of IgE, specific to the unicellular green algaChlorellasp., was analysed in sera from 46 atopic children sensitized to moulds, using radioallergosorbent test (RAST), immunoblotting and crossed immunoelectrophoresis/crossed radioimmunoelectrophoresis (CIE/CRIE).Chlorella‐specific IgE was found in 23/46 sera by RAST, in 28/41 sera by immunoblotting and in 6/30 sera by CIE/CRIE. TheChlorellacomponents most frequently binding IgE as analysed by gradient gel electrophoresis and immunoblotting were of molecular weights of approximately 13, 17, 19, 26 and 49 kD. Twenty‐nine precipitating antigens, including seven IgE‐binding precipitates were detected by CIE/CRIE. The study shows that low concentrations of specific IgE are formed to the green algaChlorellain sera from atopic individuals sensitized to m

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00523.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

*We wanted to study the effect of anti‐asthma drugs on antigen‐induced dyspnea in conscious Sprague Dawley (S.D.) rats, A line of dyspnea bred rats was produced, where all immunized animals respond with dyspnea when challenged with aerosolized oval‐bumin (OA). The animals were immunized intraperitoneally (i.p.) with OA (10 μg) together with Al(OH)3(100 mg) and challenged 2 to 3 weeks later with OA aerosol. We examined the effects of terbutaline (TERB), disodium cromoglycate (DSCG), atropine (ATRO), theophylline (THEO), a 5‐HT receptor antagonist methysergide (METH), and two glucocorticosteroids (GCS) budesonide (BUD) and dexamethasone (DEX), on this response. The drugs were given locally either by intratracheal (i.t.) instillation or by aerosol. The rats were placed one by one in an air tight box and the breathing pattern was recorded. Parameters such as time of onset of dyspnea after end of challenge (elapsed time), duration and occurrence of dyspnea, were used to quantify the response. In the vehicle‐ treated group nearly all animals responded to OA with signs of dyspnea, TERB was the only drug effective when given as an aerosol, whereas when given i.t., also DEX and METH inhibited the dyspnea. Dyspnea is a serious response and can only be alleviated by the most effective anti‐

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00524.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

We measured the IgG subclass antibody levels to wheat flour in 42 bakers and 20 controls with an enzyme immunoassay. The levels of total IgG, IgG1 IgG2 and IgG4 antibodies were significantly higher in the bakers than in the unexposed controls. The presence of anti‐wheat flour IgG subclass antibodies in the bakers was correlated with various clinical variables including IgE levels, duration of asthmatic or rhinitis symptoms, skin prick test response, peripheral blood eosinophil levels, bronchial histamine reactivity and responses to nasal challenge with wheat flour. The IgG subclass antibody levels of the total cohort of bakers did not correlate with any of the measured clinical variables. However, among men specific IgG4 and IgG1 antibody levels correlated negatively with total IgF levels and duration of rhinitis, respectively. We conclude that IgG and IgG subclass levels to wheat flour in bakers reflect exposure, but that it is not related to any specific clinical situation. The exact pathogenetic role of these antibodies in the development of occupational asthma and rhinitis is thus not clea

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00525.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

In a 2‐year double‐blind placebo controlled study an immunological evaluation was carried out on 33 patients (15 males, 18 females, mean age 29.2 years) with mite‐induced perennial rhinitis who were submitted to specific immunotherapy (IT) with an alginate‐conjugated extract ofD. pteronyssinus.The behaviour of IgE, IgG, IgG1, and IgG4, antibodies specific toD. pteronyssinusand its major allergen Der p1, was characterized by assessment of their changes m serum, and changes in IgG in nasal secretions during the treatment. The placebo‐treated patients did not show any significant variation in the levels of specific antibodies, while in the actively treated patients we found; a statistically significant decrease (P<0.005) of specific IgE, a statistically significant increase of specific IgG (P<0.005), IgG1, (P<0.005) and IgG4(P<0.005) in serum and a statistically significant increase (P<0.001) of specific IgG in nasal secretions. The IgG response showed an early relative predominance of the IgG1subclass and a late absolute predominance of IgG4subclass, that confirmed the model of IgG4restriction in prolonged allergen stimulation. No correlation was found between immunological and clin

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00526.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Information on hay fever, eczema, urticaria, respiratory symptoms, smoking habits and occupational dust or gas exposure was obtained by a self‐administered questionnaire from a random sample of 4992 subjects of the general population aged 15–70 years of the Hordaland county, Norway. The response rate was 90% of the sample. The lifetime prevalences of hay fever, eczema and urticaria were 10%, 25% and 9% of the respondents, respectively. Eczema and urticaria were more often reported by women than by men. The lifetime prevalence of hay fever decreased substantially by age in both sexes. A history of hay fever was inversely related to cigarette smoking. The lifetime prevalences of eczema and urticaria were associated with occupational dust or gas exposure after adjusting for sex, age, smoking habits and area of residence. Adjusted odds ratios of respiratory symptoms in subjects with hay fever were almost 2.0 compared with those with

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00527.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Platelet glutathione peroxidase (GSH‐Px) activity and serum selenium (Se) levels were determined in 20 patients with intrinsic asthma. Nine of the patients had NSAID‐intolerance. The mean value of GSH‐Px activity in the patients was 47.0±7.1 U/1011platelets, which is significantly lower than that of 56.4±12 U/1011platelets in the controls (P<0.01). There was also a tendency towards lowered Se levels in the patients compared with controls. The results are discussed in view of the protective role of GSH‐Px against oxidative stress and the tentative regulatory function of GSH‐Px in arachidonic acid

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00528.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

One high potency (HP) and two low potency (LP) commercial whole short ragweed (WSR) extracts were assayed for relative potency (RP) by antigen E (AgE‐Amb a 1), RAST inhibition, and parallel line bioassay (PLBA). The RP of the HP extract (300 (μg AgE) was equal to the reference WSR, but the LP extracts were only ∼ 0.01 of the RP of the reference. Each extract was administered to fall hay fever patients with skin sensitivity to WSR (≤ 10−3μg/ml AgE for sum of erythema = 50 mm) to the maximum tolerated dose, or 0.5 cc of the concentrate, by either a 9‐ or 40‐dosc regimen. HP‐treated patients had significantly lower symptom scores than untreated controls. No significant difference was noted in the total symptom scores between HP and LP extract‐treated patients. Despite ∼ 100‐fold differences in the RP of HP versus LP extracts, both produced similar frequencies of severe systemic and severe late phase local reactions. Furthermore, when RP of HP and LP extracts were estimated by PLBA, both reached similar peak dosages, similar dosage for IgG WSR antibody response, and comparable IgG WSR antibody levels, indicating that the WSR dose estimates based on RP were bioequivalent. The 9‐dosc HP regimen produced fewer late phase local and systemic reactions per patient than the 40‐dose HP regimen. Most patients experiencing severe systemic reactions were among those most skin sensitive to WSR. The number of systemic reactions per injection was significantly higher at higher WSR doses. One LP extract produced a high incidence of systemic reactions in the initial three injections. With respect to HP, its RP varied ∼ 10‐fold depending on the skin sensitivity of the patient to heat‐stable ragweed allergens, demonstrating that it was compositionally different. However, when the dose of this extract was estimated by RP based on the skin sensitivity of each patient, the peak dose of the extract as well as the doses associated with late phase and systemic reactions were found to be similar to the HP doses. This indicated that immunizing dose estimates of compositionally different WSR extracts based on RP

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00529.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Seventeen children with challenge‐verified cow milk allergy/intolerance (CMAI), age 3–78 months, median 12 months, were re‐challenged with cow milk in increasing doses. All subjects developed symptoms, such as bronchospasm, rhinitis, diarrhoea, erythema or eczema. Blood samples were taken before and up to 24 h after the start of the challenge. The cow milk protein β‐lactoglobulin (BLG) was determined in serum with ELISA (lower detection limit 0.3 μg/l). BLG was detectable in five children at low levels (below 2 μg/l). Analysis of the size distribution of the BLG by size exclusion chromatography indicated immunoreactive material as small aggregates. Plasma samples were analysed by electro‐immunoassay for complement factor split product C3d, which was not demonstrable above background values in any of the cases. CMAI in infants and children may not be related to systemic activation of the complement system and may be elicited without considerable amounts of immunoreactive BLG in the

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00530.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Twelve healthy volunteers were randomly allocated to receiving in a double‐blind cross‐over fashion levocabastine eye drops (0.5 mg/ml solution) and placebo. They were advised to instill 2 drops per eye, four times daily. Each treatment was administered for a period of 1 week. Before and after each treatment period psychomotor function was assessed using Critical Flicker Fusion Test and the Choice Reaction Time Test. At the same time intervals a subjective evaluation of sedation was given using a Visual Analogue of Sedation. Both objective and subjective measurements showed that no significant treatment effects could be detected. It is concluded that repeated instillations of levocabastine eye drops are devoid of any sedative side effe

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00531.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A patient with allergic asthma and urticaria due to heroin powder is reported, and details of bronchial provocation and other related tests are discussed.

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00532.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY