摘要:

The relation between allergy to house dust mites and allergy to storage mites was studied in 326 patients with bronchial asthma, A positive RAST to house dust mites was found in 24,5% and to storage mites in 7,4%, There was a close association between a positive RAST to house dust mites and a positive RAST to storage mites, and 91,6% had a positive RAST to both. Only 0,9% with a negative RAST to house dust mites had a positive RAST to storage mites, whereas the frequency was 27,5% in those with a positive RAST to house dust mites. Present occupation with exposure to storage mites was found in 20,8% with a positive RAST to storage mites, A positive RAST to both house dust mites and storage mites was significantly associated with anamnestic indicators of damp housing conditions. The close association between allergy to house dust mites and allergy to storage mites in this study is probably caused by a common environmental factor, i.e, damp housing conditions.

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00462.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The efficacy and safety of loratadine, a new orally active specific H‐receptor blocking antihistamine with poor penetration into the CNS, was evaluated in a double blind comparative study. One hundred and seven hay fever patients, sensitive to birch pollen, were randomized into three parallel groups receiving loratadine 40 mg once daily, clemastine 1 mg twice daily, or placebo during the birch pollen season. Both active treatments showed reduction of symptoms in comparison with placebo, but the results were more pronounced with loratadine treatment, which significantly reduced the overall allergic condition as well as all separate allergic rhino‐conjunctivitis symptoms except nasal stuffiness. Compared with placebo the sedation rate was significantly higher with clemastine treatment (P<0.05) but not with loratadine, Loratadine was thus concluded to be efficacious in hay fever treatment with a sedation rate not differing from plac

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00463.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Sera from 204 adult patients with chronic airways obstruction were analysed with the Phadiatop®, a new allergosorbent test with a paper disc containing the most relevant inhalant allergens, the PRIST for total IgE determinations, and a panel of seven RAST tests with the common inhalant allergens in The Netherlands as reference. The aim was to evaluate the Phadiatop as screening test in thein vitrodiagnostic procedures in an epidemiological setting. The Phadiatop was classified positive or negative according to percentage binding, total IgE was considered elevated at values>100 kU/1 and the RAST panel positive when at least one RAST result was class>1. The prevalence of inhalant atopy came to 27.9%. The predictive value of the Phadiatop for a positive RAST panel was 96.4%, and for a negative RAST panel 97.3%. For the PRIST these values were 51.9% and 87.2% respectively. The correlation between the log percentages binding of the Phadiatop and the RAST panel was 0.93. It is concluded that the Phadiatop is a valuable test for the screening of inhalant atopy, and as the percentage binding of the Phadiatop may reflect the degree of sensitization it could also be applied as a quantitative measure especially for epidemiological purposes

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00464.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Metachromatic cells in the nasal mucosa were studied in relation to symptoms in 16 schoolchildren and 11 adults with hay fever who were challenged with pollen outside the pollen season, using either a gentle scraping‐cytocentrifugation method for collection of mucosal specimens or biopsies. There was a temporary redistribution of metachromatic cells towards the mucosal surface appearing 5–24 h after challenge, with a correlation between the quantity of metachromatic cells and symptom scores. Thus, a single exposure to high doses of allergen may contribute to priming in susceptible individu

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00465.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The diagnosis of food IgE‐dependent hypersensitivity is based on the demonstration of specific IgE, completed by provocation tests. Two immunoenzymatic techniques, the Phadezym and the FAST, are compared with the Phadebas RAST, in 86 sera (23 controls, 28 from patients with a reported food allergy and 35 with a positive RAST to a food allergen). The within‐run variation coefficient of class 0–2 sera was 9% for the Phadebas RAST, and higher than 20 % for the Phadezym and the FAST. It was in order of 8.7%, 9.4% and of 17.6%>respectively for Phadebas RAST, Phadezym and FAST when estimated with class 3–4 sera. The specificity was higher than 95 % for the three techniques. The sensitivity was 75% for Phadebas and 43% for Phadezym and FAST. The FAST test is much less sensitive with allergens of vegetal origin than those of animal origin (P<0.01). This work indicates the high percentage of false negative results of immunoenzymatic techniques when food extracts are tested. This could be explained either by an enzyme‐substrate reaction or by a non‐specific inhibition of the enzyme linked to the a

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00466.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The ability of basophils from patients with allergic rhinitis, extrinsic asthma, chronic idiopathic urticaria and atopic dermatitis to release histamine spontaneouslyin vitrowas studied. Spontaneous release in the presence and absence of 30% D2O was investigated from both washed leukocytes and whole blood. Compared with controls histamine release (HR) from washed leukocytes was significantly enhanced in allergic rhinitis patients only, whereas in the other groups only a certain percentage of patients was found to have high spontaneous HR. In whole blood experiments HR in all groups was within the normal range. Our data indicate that enhanced spontaneous mediator release from washed basophilsin vitrodoes not necessarily prove this mechanism to be of patho‐physiological relevancein viv

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00467.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The development of allergen during metamorphosis of chironomids,Chironomus yoshimatsuiandTokunagayusurika akamusi, was studied by means of ELISA inhibition with pooled serum containing high titer of specific IgE to each adult midge. Extracts ofC. yoshimatsuilarvae and pupae did not inhibit the specific IgE antibody to adultC. yoshimatsui.Mature adultC. yoshimatsuiextract had about 10 times more inhibitory substance than the young adult. Hemoglobin, however, was degraded during the metamorphosis as measured by spectrophotometry and HPLC. The inhibitory substance against specific IgE antibody to adultT. akamusiwas identified as highest in female body, less in egg, and even less in wing, leg and male body. The size of the substance in egg and female body was estimated at>500 kD of molecular weight.

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00468.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The intention was to clarify, experimentally, some of the clinical properties of capsaicin. The modulation of histamine‐induced immediate inflammatory responses following subsequent topical applications of capsaicin was studied. A gradusd dose‐dependent reduction of the acute inflammatory reaction was observed after daily epicutaneous application of 1% capsaicin for 6 days. The modulation suggests that the small nerve fibres responsible for the neurogenic spread of the flare gradually become less excitable. After termination of capsaicin applications the flare returned to its initial value after 9 days. The recovery phase was longer than the inhibition phase, probably due to slow repletion of neuropeptides in the cutaneous nerve endi

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00469.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The study comprised 28 patients from the centre of Spain (Madrid and surroundings) who had suffered systemic reactions when stung by vespids. Specific IgE antibodies toVespulaspp. andPolisiesspp. venoms were measured by RAST. All patients had positive RAST toVespulavenom and half of them also had positive RAST toPolistesvenom. A patient can be sensitive to both venoms due to either a clinical sensitization to both venoms, or common antigenic determinants. To differentiate these states we used a RAST inhibition assay. We could inhibitPolistesRAST with eitherPolisiesorVespulavenom to a similar degree. Inhibition ofVespulaRAST was possible withVespulavenom, but only to a limited degree withPolistesvenom. Direct RAST and RAST inhibition studies indicate that in our geographic region sensitization toVespulavenom is more common than toPolistesvenom andPolistesmight have cross‐reactivity in our patient

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00470.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Forty‐six adult asthmatics allergic toD. pteronyssinus(Dp) participated in a 2‐year study. Thirty‐one underwent hyposensitization (HS‐group). Fifteen were treated with Dp‐extract (Dp‐group), and 16 with a similar extract modified by monomethoxypolyethylene glycol with reduced allergenicity (mPEG‐Dp‐group). Fifteen patients served as controls. Dp‐specific antibodies and histamine release from blood basophils were determined and compared with Dp‐sensitivity in lungs and skin. In addition, IgG and IgE against the major allergenDer pI were followed in a subgroup. Dp‐specific IgG, IgG., and IgG4increased significantly in both HS‐treated groups after 1 and 2 years (median; 2.5‐ to 11.6‐fold). IgG4was not induced if maintenance dose during the first year was less than 20,000 BU. Median skin sensitivity decreased 4.4‐ to 8.2‐fold after 1 year and 7.4‐ to 21.4‐fold after 2 years.Der pI specific IgG response was unrelated to the occurrence or change in IgE with the same specificity. The mPEG‐Dp‐extract tended to have less effect on skin sensitivity and immunological parameters, differences reaching statistical significance for skin sensitivity only. In the HS‐group, the decrease in bronchial sensitivity was significantly correlated to a decrease in IgE (r = 0.36), IgG1/IgG4(r = 0.49), Dp‐specific histamine release (r = 0.58), and to an increase in Dp‐specific IgG, (r =−0.36) and IgG4/IgE (r =−0.48). In patients improving clinically. Dp‐specific IgG4/IgE increased, and median Dp‐specific IgE was reduced to 80 % compared with an increase to 150–160% seen in the unchanged or deteriorated group (P<0.05). Findings indicate an improvement of effect, if the allergen dose is sufficient to reduce specif

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1990.tb00471.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY