ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1984.tb00861.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Rats of BN×Wi/Fu strain were immunized by daily subcutaneous (S.C.) injections of antigen without the use of adjuvant for two 2‐week periods with a 4‐week interval. The bronchial responses to airway and intravenous (i.v.) antigen challenge were measured during and after the immunization period. These responses were compared with both the humoral and the cell‐mediated immune response of the animals. Immunization induced bronchial reactivity of the animals to antigen after airway and i.v. challenge. This reactivity persisted for 7 weeks following the second immunization period. Specific IgE antibodies were detected in serum, bronchial fluid and in supernatants from cultured peripheral lymph node cells. The immunization also resulted in an IgG antibody response. Neither the amount of IgE. IgG2a, nor of any other isotype, correlated with the bronchial reactivity in the animals. Antigen simulation of lymph node cells mixed with syngeneic spleen cells induced proliferation. This procedure also resulted in a maturation of mast cells in 3‐week cultures. The immunization also resulted in some increase in the number of mast cells around vessels in the lung. There was no correlation between these parameters of cell‐mediated immunity and either antibody responses or bronchial

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1984.tb00862.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

AD. pteronyssinus, whole culture allergen preparation contained 49 antigens as revealed by crossed immunoelectrophoresis (CIE), using polyspecific rabbit antibodies. Crossed radio‐immunoelectrophoresis (CRIE) with sera from 30 patients revealed nine allergens, antigens 42, X, Y and 23 (in rank order) showing the most frequent and intense IgE‐uptake. Nine antigens originated from the culture medium (human dander + yeast), but none of these gave rise to specific IgE‐uptake. Extremely few and weak reactions were observed in radioallergosorbent (RAST) with 129 sera, using media extracts on the discs. Purified mite body extract (PMB) contained less ag 42 and more ag Y and ag 23 than whole mite culture extract (WMC), whereas an acetone‐extracted mile excreta preparation (AML) contained 5 times more ag 42, but was devoid of ag Y and ag 23. Ag X was present in all preparations. The RAST‐inhibitory potency of PMB was best correlated with the content of ag X. Preparations with properties similar to WMC and PMB were judged as suitable for clinical ap

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1984.tb00863.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1984.tb00864.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

In a study of immunotherapy 41 children with seasonal rhinoconjunctivitis due to deciduous tree pollen allergy were monitored by means of symptom scoring, patient self‐evaluation, conjunctival provocation tests and lymphocyte proliferationin vitroto the allergen. The lymphocyte responsiveness to birch pollen decreased significantly during the first year of immunotherapy. However, neither the lymphocyte responsiveness before treatment nor changes in lymphocyte reactivity during the immunotherapy correlated with the clinical efficacy of the therapy as evaluated by changes in symptom scores, self‐evaluation or conjunctival provocation test changes in the individual patients. The results indicate the lymphocyte responsiveness to an allergen cannot be used to select patients for immunotherapy, i.e. to predict whether a patient would benefit from immunotherapy or not, or to evaluate the effects of immunotherapy after beginning the treatment. However, lymphocyte proliferation response to an allergen indicates clinical sensitiv

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1984.tb00865.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Peripheral blood lymphocyte (PBI) transformation by allergens. PPD antigen and PHA mitogen, was followed in 20 allergic patients with grass pollen rhinitis, during and after a preseasonal immunotherapy. Ten patients were treated with Allpyral® and 10 with Conjuvac® two‐grass mixture. Lymphocyte proliferative responses to grass antigens decreased during therapy for most of the patients. Higher values than before treatment were seen in August. Three patient:, had increased values during the preseasonal treatment. One stopped treatment because of discomfort, the other two got urticaria during the ensuing winter seasons. No effect of immunotherapy was seen on the PPD or PHA response of cells. Cellular changes in relation to changes in specific IgE and IgG antibodies are discus

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1984.tb00866.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

We studied 145 children aged 7 years, who had been at day‐care centers for at least 3 months during the 2 first years of their lives. The group was compared with a matched population of 145 children cared for at home. Of the 290 children, 212 participated in all phases of the study, which included a questionnaire to parents, skin prick tests with seven allergens, and a clinical examination. Day‐care‐center children had twice as much otitis media in infancy as “home” children (P<0.001), and they also tended to have more other infections. On the other hand, day‐care‐center children did not have more eczema in infancy, more cumulated atopic disease by the age of seven, or more positive skin tests than home children. When the groups were combined, an increased prevalence of cumulated atopic disease and positive skin tests was found in children with infections in infancy. We conclude that under the conditions of this study infections in infancy did not facilitate the development of hypersensitivity to allergens and atopic disease. The linked tendencies to develop infections and atopy could depend on some

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1984.tb00867.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1984.tb00868.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY