ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05036.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb04769.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05037.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb02243.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

We have studied the proteins and allergens released by rye pollen in the course of a 19‐h pollen incubation process. Nearly 40% of the total extracted proteins were collected during the first 5 min, and most of them had a molecular weight<28 kDa. Between 5 and 30 min, 15% of the proteins from total extract were released, showing in the SDS‐PAGE analysis an increase in which components moved close to 30 kDa standard. From 30 min to 19 h several extracts were collected. Electrophoretical profile of components from these extracts reveals that bands moving below 28 kDa were practically absent and those of 28 and 23 kDa became very intense. At the end of the process there was a rise of 67 kDa proteins. Dot‐immunobinding and immunoblotting techniques reveal that allergens leave the rye pollen, for the most part, after 5 min incubation and are proteins with 28 kDa, 33 kDa, 48 kDa and 67 kDa molecular we

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb02244.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Rye pollen was incubated for 30 min and proteins extracted at this time were collected as extract A (EA). The same pollen grains were resuspended in buffer and incubated for 18.5 h. Proteins extracted in this period were designated extract B (EB), Both extracts were subfractionated by DEAE ion‐exchange chromatography and allergen presence in peaks detected by the dot‐immnunobinding technique. The results reveal that unretained proteins (peaks 1 and 2) and proteins eluted at 0.2 M NaCl from extract B contain the highest proportion of allergens, SDS‐PAGE of chromatographic peaks showed that peak 2 from extract B contains a highly purified 28 kDa band. On the skin of allergic patients this band gave a stronger positive prick test than for the crude ex

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb02245.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Bronchial provocation tests (BPT) with allergen extracts were performed in 82 children with asthma, and the results were compared with the chemiluminescent assay (CLA), the radio‐allergo‐sorbent‐test (RAST) and skin prick test (SPT). It was found that CLA matched BPT with a concordance of 87% (77–93%), RAST with a concordance of 74% (64–83%) and SPT with 80% (70–88%). Most often, CLA and RAST gave similar results, although their classes did not matc

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb02246.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The aim of the present study was to compare yellow jacket venom extracts from two different companies and to compare skin prick test (SPT) with intradermal test (IDT). IDT and SPT with yellow jacket venom (ALK and Pharmacia Reless®) were performed on 54 yellow jacket allergic patients and 44 symptom‐free volunteers. Venom was diluted to 300, 100, 10 and 1 μ/ml for SPT and 100, 10−1, 10−2, 10−3and 10−4μ/ml for IDT, according to the manufacturers’instructions. Skin tests were performed on both forearms. Both extracts showed approximately the same relationship between sensitivity and specificity, but the Pharmacia Reless yellow jacket venom extract showed a 5–10 fold higher biological activity in both SPT and IDT. Thus yellow jacket venoms of ALK and Pharmacia Reless are not comparable in allergen activity at the same venom concentrations. Using extracts from the same company, SPT and IDT were comparable with regard to sensitivity and specificity at an allergen concentration 1000 times higher for

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb02247.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

To identify the allergenic components of honey we studied 22 patients with a history of systemic allergic symptoms following honey ingestion. The group of honey‐allergic patients was compared with three control groups: 10 subjects sensitized to artemisia, 10 with honey bee venom allergy and 10 without a history of atopy or bee sting reactions. The allergological tests included skin tests and RAST with three different kinds of Swiss honey (dandelion, forest and rape), pollen of compositae species, celery tuber, extract of bee pharyngeal glands, honey bee venom and bee whole body extract. The results show that 3/4 of honey‐allergies are sensitive to dandelion honey and 13 of 22 also to compositae pollen. Nine of the honey allergic patients were sensitized to honey bee venom, 3 also to bee pharyngeal glands and to bee whole body extract. Analysis of diagnostic tests and RAST inhibition studies suggest that besides compositae pollen other allergens, most likely of bee origin are important. In honey allergies primary sensitization may be due either to the honey itself, to airborne compositae pollen or even to cross‐reacting bee venom compo

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb02248.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05038.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY