ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00700.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00701.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00702.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

The distribution of IgE in a large randomly stratified Greek population sample was determined in 1187 subjects (793 men and 394 women) aged between 20 and 60 years. Skin prick testing was performed and serum total IgE expressed in iu/ml was measured by Phadebas PRIST: the data are presented as the geometric mean. Subjects were classified as atopic (257 men, 118 women) and nonatopic (536 men, 276 women) according to the results of skin testing with various aeroallergens. At any age, atopic males (120.5 vs 38 iu/ml) and females (99.8 vs 29.3 iu/ml) had higher mean IgE levels, as compared to nonatopic subjects (P0.05). At any age, nonatopic males had higher (38 iu/ml) mean IgE levels than nonatopic females (29.3 iu/ml) (P<0.05). The comparison of normal IgE values (nonatopic subjects) from this study with those reported by other investigators revealed that Greek adult males and females had higher IgE levels than populations from other nations. Our results represent the first report on reference values regarding serum total IgE in Greek adults.

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00703.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

The sensitizing capacity of brewer's yeast (Saccharomyces cerevisiae) was studied with the skin prick test method in 449 subjects, including 226 atopic dermatitis (AD) patients, 50 patients with allergic rhinitis (AR) and/or asthma (A), and 173 nonatopic controls. A positive SPT reaction (≥++) was seen in 94% of patients with severe AD, in 76% with moderate AD, and in 25% with mild AD or no history of AD. Patients with AR and/or A and nonatopic controls displayed a positive reaction in only 8 and 2% of cases, respectively. There was also a parallel skin prick test reactivity with other yeasts includingPityrosporum ovaleandCandida albicans, suggesting cross‐reactivity. Parallel skin reactivity was observed also with molds and animal dander but not with pollen or house‐dust mite. A significant correlation was also found between total serum IgE level and skin prick test (SPT) results withS. cerev

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00704.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Dose‐ and pH‐ dependent carbodiimide‐mediated coupling of Penicillin‐G to polystyrene microtiter‐plates that leaves the beta‐lactam ring unchanged is described. A new ELISA method was developed using Penicillin‐G coated plates. The binding of 3 different monoclonal antibodies as well as human IgG antibodies of the IgG1and IgG3subclasses is demonstrated, whereas IgG2, IgG4and IgE antibodies did not bind. Thus, covalently coupled Penicillin‐G can be used to study the immune‐response to the unchanged β‐lactam ring in patients receiving penicillin therapy. The new method is complementary to hitherto described techniques, which generally only allow detection of antibodies binding t

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00705.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Carpet and floor dust samples were collected in four different seasons, from 39 Swedish homes of babies with a family history of allergy. House‐dust mite (Der p 1, Der f 1) and cat (Fel d 1) allergen contents were determined by mab ELISA, and the levels were related to various environmental factors. Both mite and cat antigens were detected in 94% of the samples and in all homes, but the levels were low (Der p 1, range 15 ng–1944 ng/g fine dust;Der f 1, range 14 ng–264 ng/g of fine dust;Fel d 1, range 16 ng–3120 ng/g fine dust). Mite‐allergen levels were significantly higher (P<0.001) in floor dust than in carpets, andD. pteronyssinuspredominated. In contrast, the levels of cat antigen were significantly (P<0.05) higher in carpets than in floor dust. There was no clear relation between mite‐allergen levels and type of house, except that the higher values were found in homes with dampness problems. Cat‐allergen levels were higher than total mite‐allergen content, and the highest levels were found in homes with a cat (P<0.05). Rather high concentrations of cat allergen were also found in homes without a cat, which may explain why cat sensitization is so common in Sweden. As the prevalence of house‐dust mite sensitivity is increasing in Swedish children, and as the individual patient threshold for eliciting symptoms varies, we suggest that sensitization may possibly occur at a lower exposure level than generally accepted as risk level for sensitizat

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00706.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

The aim of this study was to evaluate the function of basophil granulocytes in 37 patients with progressive systemic sclerosis (PSS). Thein vitroproteinase release test using Chromozyme TH as a chromogenic substrate was performed to measure the basophil cell releasability. Anti‐IgE, calcium‐ionophore (A 23187) and N‐formyl‐methyonil‐leueyl‐phenylalanine were used as activatory agents. Our results showed that the reactivity pattern to those agents did not differ in PSS compared to controls. Patients whose basophil cells release proteinases to all of the 3 activators tended to have more severe organ symptoms compared to the rest of the patients, while teleangiectasia was significantly less frequently (p<0.02) found in cases with proteinase release. These findings suggest that there may be a certain relationship between basophil function and severity

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00707.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Allergy to kiwi, poppy seeds, and/or sesame seeds often occurs in patients with a simultaneous sensitization to nuts and flour. Previously cross reactions have been verified by RAST inhibition. In this study the nature of this cross‐reactivity is further characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE), followed by immunoblotting to nitrocellulose. The degree of cross‐reactivity among kiwi, sesame seeds, poppy seeds, hazelnuts, and rye grain was found to be very high in the patients studied. The existence of both cross‐reacting and unique components was observed; however, the cross‐reacting and unique components could be different for different

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00708.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A methacholine or a histamine test is often used to quantitate bronchial responsiveness, in studies investigating the influence of various inhaled stimuli on the responsiveness and composition of bronchoalveolar lavage (BAL) fluid. Histamine has been shown to evoke an inflammatory cell response in BAL fluid. In order to test the influence of the methacholine test on the cellular composition of BAL fluid, we investigated 14 healthy, nonsmoking volunteers with a methacholine test 24 h before performing a BAL. The results showed no significant changes in the cellular composition of BAL fluid, as compared with control BAL in the same persons.

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1993.tb00709.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY