摘要:

AbstractIon beam sputtering for high‐resolution electron microscopy has basically provided miscellaneous operational features such as atomic shadowing, uncoated observation, and etching of biological specimens coupled with tungsten sputter coating and thinning of solid materials.Based on the power‐potential law of Lindhard for ionic impact phenomena on metal surfaces, the universal yield‐energy relationship has been derived. Thereby the sputtering deposition rate with reference to the sputtering removal rate was obtained as a function of sputtering yield, and the most important angular distribution of sputtering yield could be measured by using the hemicylindrical specimen stage.Evidence is presented to show that ion beam sputtering has become one of the most powerful tools for high‐resolution electron mic

ISSN:0741-0581    

DOI:10.1002/jemt.1060040102

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

摘要:

AbstractRadioautography and immunocytochemistry may be combined at the light and electron microscopic levels for simultaneously localizing uptake sites for exogenous transmitter molecules [such as (3H)monoamines or (3H)amino acids] and endogenous transmitter‐related antigens (classical transmitters and their synthesizing enzymes as well as neuropeptides) in the central nervous system. Silver grain accumulations indicative of transmitter uptake sites are readily distinguishable from immunocytochemical labels of the peroxidase‐antiperoxidase (PAP), avitin‐biotin, or colloidal gold methods. The combination of uptake radioautography and immunocytochemistry may be applied to the investigation of (1) the chemical identity of (3H) transmitter‐accumulating elements, (2) the coexistence of different neurotransmitters within the same neurons, and (3) the cellular basis of interactions between certain neurotransmitters, in particular monoamines, GABA, and neuropeptides. This article describes and evaluates the method and reviews the available experimental data derived from its appl

ISSN:0741-0581    

DOI:10.1002/jemt.1060040103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

摘要:

AbstractConventional lectin cytochemistry or immunocytochemistry can be associated with freeze‐fracture in fracture‐label techniques: The thin section fracture‐label and the critical point drying fracture‐label. In the first method, which is particularly useful for investigating intracellular membranes, cells or tissues are freeze‐fractured, thawed, labeled, and embedded in resin. Wheat germ agglutinin labeling on freeze‐fractured human lymphocytes confirms the existence of two compartments of intracellular membranes, based on the sialoglycocompon

ISSN:0741-0581    

DOI:10.1002/jemt.1060040104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

摘要:

AbstractScanning electron microscopy autoradiography (SEM‐AR) in conjunction with light microscope autoradiography (LM‐AR) was used to follow the movement of125I‐labeled blueberry shoestring virus (BBSSV) through its aphid vectorIllinoia pepperiwith varying acquisition access periods (AAP). At 6 hr AAP, the virus had reached the stomach; at 12 hr AAP, it had reached the anterior of the intestines, and after 48 hr, AAP was present throughout the aphid. SEM‐AR using backscatter electron detection proved very useful because the sample bulk resulted in a shortened exposure time compared to LM sections, correlation of the autoradiograms could be made with fine structure, preparing large numbers of samples was easy, and examining whole longitudinal sections of aphids at low magnification with a clearly visible marker was possible. Limitations were mostly attributed to sample preparation and, in some cases, were easily r

ISSN:0741-0581    

DOI:10.1002/jemt.1060040105

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

摘要:

AbstractA digital image processing method for noise removal and image enhancement in nonperiodic structural images is described. The method for noise removal uses a reversible transform between an image and image autocorrelation function. The Laplacian filter is then employed for image enhancement. Furthermore, an on‐line image processing system for highresolution TEM is presente

ISSN:0741-0581    

DOI:10.1002/jemt.1060040106

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

ISSN:0741-0581    

DOI:10.1002/jemt.1060040107

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

ISSN:0741-0581    

DOI:10.1002/jemt.1060040108

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

ISSN:0741-0581    

DOI:10.1002/jemt.1060040109

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

ISSN:0741-0581    

DOI:10.1002/jemt.1060040110

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

ISSN:0741-0581    

DOI:10.1002/jemt.1060040111

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY